IL-4 and IL-10 are essential for immunosuppression induced by high molecular weight proteins from Ascaris suum

Souza, Valdênia Maria Oliveira de; Jacysyn, Jacqueline F.; Macedo, Mahasti Sahihi de

doi:10.1016/j.cyto.2004.07.004

Cited by 17 publications

(27 citation statements)

References 51 publications

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“…IL-10 has previously been shown by us [17,18] to be crucial in the suppression of Th2 and, together with IL-4, also suppresses Th1 responses in mice injected with Asc or PI. In murine Schistosoma mansoni infections [40], IL-10 also seems to be an endogenous down-regulator of type 1 and type 2 cytokine synthesis.…”

Section: Discussionmentioning

confidence: 83%

“…Analyzing the role of these Th2 cytokines in this effect, we demonstrated that, in the absence of IL-4 and IL-10, Asc or PI were unable to down-regulate Th1 or Th2 responses against OVA [17,18]. On the other hand, in vitro neutralization of IL-4 and IL-10 did not change the proliferative hyporesponsiveness and the low cytokine secretion by OVA-specific T cells obtained from mice immunized with OVA+Asc [17].…”

Section: Introductionmentioning

confidence: 89%

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Immunosuppressive components of Ascaris suum down‐regulate expression of costimulatory molecules and function of antigen‐presenting cells via an IL‐10‐mediated mechanism

et al. 2006

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High-molecular-weight components (PI) of Ascaris suum suppress both cell-mediated and humoral responses against ovalbumin (OVA) via an IL-4/IL-10-dependent mechanism. The aim of this work was to investigate the effect of PI on the ability of APC to activate T cells and the role of IL-10 in this process. Flow cytometry analyses of MHC class II, CD80, CD86 and CD40 molecules on LN cells from mice immunized with OVA or OVA+PI showed that PI inhibits expression of these molecules on unfractionated cells and on purified CD11c + cells. A low proliferative response was obtained when OVA-specific TCR-Tg T cells were incubated with CD11c + cells from OVA+PI-immunized mice pulsed with OVA, when compared to those incubated with cells from OVAimmunized mice. Similar results were obtained using as APC CD11c + cells from OVAimmunized mice pulsed with OVA+PI, which also expressed less of the four markers. The inhibitory effect of PI on both the expression of costimulatory molecules and the induction of T cell proliferation was abolished in IL-10-deficient mice. Our data indicate that the potent immunosuppressive effect of A. suum extract components on the host immune system is primarily related to their property of down-regulating the Agpresenting ability of DC via an IL-10-mediated mechanism.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 89%

Immunosuppressive components of Ascaris suum down‐regulate expression of costimulatory molecules and function of antigen‐presenting cells via an IL‐10‐mediated mechanism

et al. 2006

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“…We have shown that almost no DTH reaction is obtained in our immunization model for genetic deficiency of IL-12 production 18 . Additionally, another IL-12 family member, IL-23, is committed to the Th17-subtype mediated immune response, in which IL-17 is secreted 20 .…”

Section: Figurementioning

confidence: 99%

“…In mice, when PI was combined with ovalbumin (OVA) and adjuvant, both OVA-specific Th1 and Th2 immune responses were found to be impaired (lymphoproliferation, delayed-type hypersensitivity (DTH) reactions, IgG2a/IgG1/IgE and IL-2/ IFN-γ/IL-4/IL-10 production) 5 . Using IL-4 or IL-10 knockout mice that were immunized in the same way, we demonstrated the essential role of IL-10 in suppressing the OVA-specific Th2 immune response, while IL-10 plus IL-4 were required for suppressing Th1-related parameters 18 . In IL-12 or IFN-γ knockout mice, the PI suppressive ability remained intact.…”

mentioning

confidence: 99%

“…In IL-12 or IFN-γ knockout mice, the PI suppressive ability remained intact. Unexpectedly, OVA-immunized IFN-γ-deficient mice developed a very intense OVA-specific DTH reaction, that seemed to be more strongly impaired when these mice received PI 18 . Thus, in the present study, we investigated the type of infiltrating cell in an OVA-specific DTH and analyzed the intensity of the suppressive effect of PI in the absence of IFN-γ.…”

mentioning

confidence: 99%

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Boosting the suppressive effects of Ascaris suum components in IFN-γ-deficient mice

Souza

Macedo

2009

Rev. Soc. Bras. Med. Trop.

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High molecular weight components from Ascaris suum extract suppress ovalbumin-specific immunity in mice. In IFN-γ-deficient mice, ovalbuminspecific delayed-type hypersensitivity reactions are more strongly downregulated by these suppressive components. Here, the cellularity of the delayed-type hypersensitivity reaction in IFN-γ-deficient mice and the increased downregulation induced by Ascaris suum components were analyzed. IL-12p40-dependent neutrophilic influx was predominant. Suboptimal doses of the suppressive fraction from this nematode completely inhibited the hypersensitivity reaction, thus indicating intensification of the immunosuppression under conditions of intense recruitment of IFN-γ-independent neutrophils. Key-words:Ascaris suum. Delayed-type hypersensitivity. Immunosuppression. IFN-γ-deficient mice. Neutrophil. RESUMOComponentes de alto peso molecular do extrato de Ascaris suum suprimem a imunidade específica à ovalbumina em camundongos. Em camundongos geneticamente deficientes de IFN-γ a reação de hipersensibilidade tardia específica para ovalbumina foi mais fortemente prejudicada por estes componentes supressivos. Aqui, a celularidade da reação de hipersensibilidade tardia em camundongos deficientes de IFN-γe o incremento na supressão induzida por componentes do Ascaris suum foram analisados. Influxo neutrofílico, dependente de IL-12p40, foi predominante. Dose subótima da fração supressiva do nematódeo inibiu completamente a reação de hipersensibilidade, indicando uma intensificação da imunossupressão em condições de recrutamento intenso de neutrófilos independente de IFN-γ.  Palavras-chaves: Ascaris suum. Hipersensibilidade tardia. Imunossupressão. Camundongos deficientes em IFN-γ. Neutrófilos.

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Neutralization of Th2 Cytokines in Therapy of Cardiovascular Pathology

Mandel¹,

Bolton²

2007

Immune Dysfunction and Immunotherapy in Heart Disease

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IL-4 and IL-10 are essential for immunosuppression induced by high molecular weight proteins from Ascaris suum

Cited by 17 publications

References 51 publications

Immunosuppressive components of Ascaris suum down‐regulate expression of costimulatory molecules and function of antigen‐presenting cells via an IL‐10‐mediated mechanism

Immunosuppressive components of Ascaris suum down‐regulate expression of costimulatory molecules and function of antigen‐presenting cells via an IL‐10‐mediated mechanism

Boosting the suppressive effects of Ascaris suum components in IFN-γ-deficient mice

Neutralization of Th2 Cytokines in Therapy of Cardiovascular Pathology

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