IL-8 Gene Variants and Expression in Childhood Asthma

Charrad, R; Kaabachi, Wajih; Rafrafi, Ahlem; Berraies, A.; Hamzaoui, Kamel; Hamzaoui, A.

doi:10.1007/s00408-017-0058-6

Cited by 30 publications

(25 citation statements)

References 40 publications

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“…IL-8 has previously been identified as a modifier gene influencing lung function in CF [ 43 , 44 ]. Indeed, IL-8 may have a broader role in lung health as links have been found between IL-8 and lung function measures in COPD [ 45 ], wheezing in infancy [ 46 ] and asthma in childhood [ 47 ]. It is biologically plausible that more IL-8 leads to enhanced recruitment of activated neutrophils with resultant release of more elastase causing progressive lung destruction.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory epithelial cytokines afterin vitrorespiratory syncytial viral infection are associated with reduced lung function

et al. 2021

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Respiratory Syncytial Virus (RSV) infections in early life predispose children with Cystic Fibrosis (CF) to more severe lung function decline in later life. The mechanisms explaining the associations between RSV and progression of CF lung disease are not clear.In this study, a human bronchial epithelial cell line (HBE) and primary human nasal epithelial cells (PNECs) from individuals with CF and healthy control donors were infected with RSV. RT-PCR, plaque assay, cytokine detection, immunofluorescence and Western blot analyses were performed.RSV replicated to higher level in CF epithelial cells as compared to control cells, however no defects in innate immune pathways were identified in CF cells. Rather, primary p.Phe508del CFTR PNECs produced more cytokines after RSV infection than control cells. Moreover, IL8 and TNF-α production post RSV negatively correlated with lung function (% predicted forced expired volume in 1 s or FEV1) in the individuals who donated the cellsThese data suggest that CF epithelium has a dysfunctional response to RSV allowing for enhanced viral replication and an exaggerated inflammatory response that ultimately may predispose to greater airway inflammation and reduced lung function.

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Section: Discussionmentioning

confidence: 99%

Inflammatory epithelial cytokines afterin vitrorespiratory syncytial viral infection are associated with reduced lung function

et al. 2021

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“…This was also consistent with previous reports that the expression level of IL-8 was significantly elevated in children with asthma. [31][32][33][34] Next, we assessed the expression levels of LncRNAs in T cells, B cells, and monocytes among PBMCs. We found that LOC643072 (of unknown function) was mainly expressed in monocytes (Fig 1, D).…”

Section: Lnc-baz2b Is Upregulated In Children With Asthmamentioning

confidence: 99%

lnc-BAZ2B promotes M2 macrophage activation and inflammation in children with asthma through stabilizing BAZ2B pre-mRNA

Wang

Liu

et al. 2021

Journal of Allergy and Clinical Immunology

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“…Numerous genetic and molecular studies have been carried out in the eld of asthma in both the children and in adults previously [50][51][52][53][54][55][56][57][58][59][60]. Among genetic studies, exome/NG sequencing studies has been documented throughout the global populations [61,62].…”

Section: Resultsmentioning

confidence: 99%

Next Generation Exome Sequencing of paediatric Asthma Identifies Rare and Novel Variants in Candidate Genes

Bogari

Amin

Rayes

et al. 2020

Preprint

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Background: Multiple genes have been implicated to have a role in asthma predisposition by association studies. Paediatric patients often manifest more extensive disease and a particularly severe disease course. It is likely that genetic predisposition could play a more substantial role in this group. This study aims to identify the spectrum of rare and novel variation in known paediatric asthma susceptibility genes using whole exome sequencing-analysis in nine individual cases of childhood onset allergic asthma. Data were processed through an analytical pipeline to align sequence reads, conduct quality checks, identify and annotate variants where patient sequence differed from the reference sequence. Results: DNA samples from the nine children with a history of bronchial asthma diagnosis underwent targeted exome capture and sequencing. For each patient, the entire complement of rare variation within strongly associated candidate genes was catalogued. The analysis showed 21 variants in the subjects, 13 had been previously identified and 8 were novel. Also, amongst of which, nineteen were non-synonymous and 2 were nonsense. With regard to the novel variants, the 2 non-synonymous variants in the PRKG1 gene (PRKG1: p.C519W and PRKG1: p.G520W) were presented in 4 cases, and a non-synonymous variant in the MAVS gene (MAVS: p.A45V) was identified in 3 cases. The variants we found in this study will enrich the variants spectrum and build up the database in the Saudi population. Novel eight variants were identified in the study which provides more evidence in the genetic susceptibility in asthma among Saudi children.Conclusion: Screening a cohort of Saudi children for molecular identification of polymorphisms associated with allergic asthmatic response. These, together with the clinical phenotypes, revealed genetic determinants for paediatric asthma and also we compared to the similar previous reports. Providing a genetic screening map for the molecular genetic determinants of allergic disease in Saudi children, with the goal of reducing the impact of chronic diseases on the health and the economy. We belief that the advanced specified statistical filtration/annotation programs used in this study succeeded to release such results in preliminary study, exploring the genetic map of that disease in Saudi children.

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IL-8 Gene Variants and Expression in Childhood Asthma

Abstract: Both IL-8 gene and protein expression may play a key role in asthma pathogenesis.

Cited by 30 publications

References 40 publications

Inflammatory epithelial cytokines afterin vitrorespiratory syncytial viral infection are associated with reduced lung function

Inflammatory epithelial cytokines afterin vitrorespiratory syncytial viral infection are associated with reduced lung function

lnc-BAZ2B promotes M2 macrophage activation and inflammation in children with asthma through stabilizing BAZ2B pre-mRNA

Next Generation Exome Sequencing of paediatric Asthma Identifies Rare and Novel Variants in Candidate Genes

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