Ilececum: A Comprehensive Review

Tang, Shou‐jiang; Wu, Ruonan

doi:10.1155/2019/1451835

Cited by 14 publications

(9 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, our technique can be applied to remove contaminating SM and ILF from endoscopic biopsies (Extended Data Figure 3A-B), for the cases in which this occurs 13,14 . Furthermore, advanced endoscopic visualization methods can identify ILF 20 , which could be assessed for composition and function using a modified version of this method. This would be useful for assessing other patient cohorts, such as IBD patients on distinct treatment regimens.…”

Section: Limitationsmentioning

confidence: 99%

Identification, isolation and analysis of human gut-associated lymphoid tissues

et al. 2021

View full text Add to dashboard Cite

General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research.  You may not further distribute the material or use it for any profit-making activity or commercial gain  You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.

show abstract

Section: Limitationsmentioning

confidence: 99%

Identification, isolation and analysis of human gut-associated lymphoid tissues

et al. 2021

View full text Add to dashboard Cite

show abstract

“…We firstly focused on the terminal ileum which is frequently affected by inflammation in the intestine [ 18 ]. We used a distal part of the small intestine; 3-cm length from the terminal ileum in adult mice and a distal 1/3 part between the cecum and pyloric ring in fetal mice at embryonic day 16 (E16) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Collagen type I-mediated mechanotransduction controls epithelial cell fate conversion during intestinal inflammation

et al. 2022

View full text Add to dashboard Cite

Background The emerging concepts of fetal-like reprogramming following tissue injury have been well recognized as an important cue for resolving regenerative mechanisms of intestinal epithelium during inflammation. We previously revealed that the remodeling of mesenchyme with collagen fibril induces YAP/TAZ-dependent fate conversion of intestinal/colonic epithelial cells covering the wound bed towards fetal-like progenitors. To fully elucidate the mechanisms underlying the link between extracellular matrix (ECM) remodeling of mesenchyme and fetal-like reprogramming of epithelial cells, it is critical to understand how collagen type I influence the phenotype of epithelial cells. In this study, we utilize collagen sphere, which is the epithelial organoids cultured in purified collagen type I, to understand the mechanisms of the inflammatory associated reprogramming. Resolving the entire landscape of regulatory networks of the collagen sphere is useful to dissect the reprogrammed signature of the intestinal epithelium. Methods We performed microarray, RNA-seq, and ATAC-seq analyses of the murine collagen sphere in comparison with Matrigel organoid and fetal enterosphere (FEnS). We subsequently cultured human colon epithelium in collagen type I and performed RNA-seq analysis. The enriched genes were validated by gene expression comparison between published gene sets and immunofluorescence in pathological specimens of ulcerative colitis (UC). Results The murine collagen sphere was confirmed to have inflammatory and regenerative signatures from RNA-seq analysis. ATAC-seq analysis confirmed that the YAP/TAZ-TEAD axis plays a central role in the induction of the distinctive signature. Among them, TAZ has implied its relevant role in the process of reprogramming and the ATAC-based motif analysis demonstrated not only Tead proteins, but also Fra1 and Runx2, which are highly enriched in the collagen sphere. Additionally, the human collagen sphere also showed a highly significant enrichment of both inflammatory and fetal-like signatures. Immunofluorescence staining confirmed that the representative genes in the human collagen sphere were highly expressed in the inflammatory region of ulcerative colitis. Conclusions Collagen type I showed a significant influence in the acquisition of the reprogrammed inflammatory signature in both mice and humans. Dissection of the cell fate conversion and its mechanisms shown in this study can enhance our understanding of how the epithelial signature of inflammation is influenced by the ECM niche.

show abstract

“…With increased colon cancer screening, appendiceal and/or cecal lesions involving the appendiceal orifice are becoming more frequently encountered 15 . Surgery, including right hemicolectomy and partial cecectomy, has often been used as the standard therapy for these lesions.…”

Section: Discussionmentioning

confidence: 99%

Endoscopic transcecal appendectomy: a new endotherapy for appendiceal orifice lesions

Guo

Feng

et al. 2021

Endoscopy

View full text Add to dashboard Cite

Background Endoscopic transcecal appendectomy (ETA) has been reported as a minimally invasive alternative procedure for lesions involving the appendiceal orifice. The aim of this case series study was to evaluate the feasibility, safety, and effectiveness of ETA for lesions at the appendiceal orifice. Methods This retrospective study included consecutive patients with appendiceal orifice lesions who underwent ETA between December 2018 and March 2021. The primary outcome was technical success. The secondary outcomes included postoperative adverse events, postoperative hospital stay, and recurrence. Results 13 patients with appendiceal orifice lesions underwent ETA during the study period. The median lesion size was 20 mm (range 8–50). Lesions morphologies were polypoid lesions (n = 5), laterally spreading tumors (n = 4), and submucosal lesions (n = 4). Technical success with complete resection was achieved in all 13 cases. There were no postoperative bleeding, perforation, or intra-abdominal abscess. The median length of hospital stay after ETA was 8 days (range 6–18). There was no tumor recurrence during a median follow-up of 17 months (range 1–28). Conclusions ETA is feasible, safe, and effective for complete resection of appendiceal orifice lesions. Larger, multicenter, prospective studies are needed to further assess this technique.

show abstract

Ilececum: A Comprehensive Review

Cited by 14 publications

References 91 publications

Identification, isolation and analysis of human gut-associated lymphoid tissues

Identification, isolation and analysis of human gut-associated lymphoid tissues

Collagen type I-mediated mechanotransduction controls epithelial cell fate conversion during intestinal inflammation

Endoscopic transcecal appendectomy: a new endotherapy for appendiceal orifice lesions

Contact Info

Product

Resources

About