Image analyses of collagen types and thickness in oral sub mucous fibrosis stained with picrosirius red under polarizing microscope

Kamath, Venkatesh V; Satelur, Krishnanand; Komali, Y; Krishnamurthy, Shruthi

doi:10.4103/0975-8844.124258

Cited by 12 publications

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“…The study of the level of fibrosis using H and E and special stains using Light Microscope was similar to the studies by Gupta et al, [5] and Joseph et al [10] Polarizing microscopy was used to study the type of collagen using picrosirius red stain showed that Type I & Type III collagen types are seen more in majority of cases by both the observerswith a significant p value (< 0.01). The finding of the present study was in line with the study by Kamath et al, [16,17] who correlated the type of collagen with the functional and histological grading.…”

Section: Resultssupporting

confidence: 93%

Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

Ikram

Jeddy

2017

IJMDS

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Section: Resultssupporting

confidence: 93%

Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

Ikram

Jeddy

2017

IJMDS

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“…Thickness, density of packing, and spatial arrangement determine the polarization birefringence of the collagen. PSR staining thus helps in better understanding collagen function and pathology . In addition, when observed under a polarizing microscope, the PSR sections showed birefringence due to the anisotropic properties of collagen …”

Section: Discussionmentioning

confidence: 97%

Histochemical analysis of collagen reorganization at the invasive front of oral squamous cell carcinoma tumors

Alrani

Acharya

et al. 2017

J of Invest & Clin Dent

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“…The stained sections showing stromal collagen fibers in the three experimental groups were captured with a camera with a 40x objective. Fiber thickness was determined on the image captured, using image analysis software (ImageJ) [8].…”

Section: Figure 3: Herbal Gel Topical Applicationmentioning

confidence: 99%

Efficacy of Low-level Laser Therapy, Hyaluronic Acid Gel, and Herbal Gel as Adjunctive Tools in Gingivectomy Wound Healing: A Randomized Comparative Clinical and Histological Study

Reddy

Koduganti

Panthula

et al. 2019

Cureus

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IntroductionGingival overgrowth is usually an inflammatory response to plaque present on tooth surfaces. The other causes could be drugs and other systemic conditions. When the local factors are responsible and subgingival scaling does not help, gingivectomy is performed. The gingivectomy wound is raw and heals slowly. Lowlevel laser therapy (LLLT), hyaluronic acid, and herbal gels aid in healing after a gingivectomy. This study compared the efficacy of LLLT, hyaluronic acid, and herbal gel when used topically after a gingivectomy. This was a single-arm, interventional trial wherein 30 patients aged between 18 and 40 years with moderate gingival overgrowth participated. The study was approved by the institutional ethical committee. (DN/0109-16). The participants signed the consent form and the study was also registered (NCT03569683). Materials and methodsThe samples were equally divided into three groups. Group A received LLLT immediately postop, and on the first, third, and seventh days after surgery. Group B received hyaluronic acid (Gengigel) while Group C received an herbal gel (Hiora SG) for the same time periods after surgery, respectively. Analysis of variance (ANOVA) followed by a post-hoc Bonferroni test was used to evaluate differences within groups. Intergroup comparison was performed using the independent t-test. A p-value of <0.05 was considered statistically significant. ResultsThe plaque index (PI), gingival index (GI), and gingival enlargement index (GEI) showed good improvement postoperatively within the groups, which was statistically significant. However, on an intergroup comparison, the GEI pertaining only to the laser group showed significant changes. Also, the pain perception analyzed by the visual analog score (VAS) was the least, and histologically, the amount of mature collagen fibers laid down were more in the laser group. ConclusionPatients irradiated with laser after gingivectomy (Group A) showed superior results in the clinical, histological variables as compared to Groups B and C.

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Image analyses of collagen types and thickness in oral sub mucous fibrosis stained with picrosirius red under polarizing microscope

Cited by 12 publications

References 0 publications

Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

Histochemical analysis of collagen reorganization at the invasive front of oral squamous cell carcinoma tumors

Efficacy of Low-level Laser Therapy, Hyaluronic Acid Gel, and Herbal Gel as Adjunctive Tools in Gingivectomy Wound Healing: A Randomized Comparative Clinical and Histological Study

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