Image analysis assisted study of mitotic figures in oral epithelial dysplasia and squamous cell carcinoma using differential stains

Tandon, Ankita; Singh, Narendra Nath; Brave, V R; Sreedhar, Gadiputi

doi:10.1016/j.jobcr.2016.09.003

Cited by 12 publications

(14 citation statements)

References 37 publications

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“…The rate of mitosis in psoriasis is high [9] (>6/hpf) and is comparable with that of oral SCC [10] (mean mitotic figures/hpf -6.3); thus, psoriasis, which is itself a hyperproliferative disorder, is probably regulated to some extent, so that it does not reach to a level of malignancy, though it can still go on to a stage of exfoliative dermatitis. What could be the responsible factors, which are at play in inhibiting this conversion, needs to be explored further.…”

Section: Discussionmentioning

confidence: 91%

Squamous Cell Carcinoma Developing De Novo in an Untreated, Nonsun-Exposed Psoriatic Plaque

Tandon

Sharma

Zutso

et al. 2019

Indian Journal of Medical and Paediatric Oncology

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Psoriasis is a chronic inflammatory dermatosis, which causes hyperproliferation of skin. Patients afflicted with psoriasis are also at a risk of developing nonmelanoma skin cancers, with the risk of such a possibility being attributed to the administration of systemic immunosuppressive agents, phototherapy, and biological agents. We present a case of psoriasis with de novo development of squamous cell carcinoma, in a photoprotected site, with no history of administration of systemic immunosuppressive agents, phototherapy, or biological agents.

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Section: Discussionmentioning

confidence: 91%

Squamous Cell Carcinoma Developing De Novo in an Untreated, Nonsun-Exposed Psoriatic Plaque

Tandon

Sharma

Zutso

et al. 2019

Indian Journal of Medical and Paediatric Oncology

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“…Although hairy‐like projections were considered the cornerstone feature in identifying mitoses in LM 6,21 these projections are not obvious in approximately 60% of mitotic figures in WSIs. In previous studies, assessing mitoses in WSI depended upon existing criteria to define mitosis in LM, which include absent nuclear membrane and clear hairy‐like projections at the outline of the nucleus 4,21 . However, the lack of fine‐tuning facility in whole slide scanners makes identifying these fine details a challenging task.…”

Section: Discussionmentioning

confidence: 99%

Visual assessment of mitotic figures in breast cancer: a comparative study between light microscopy and whole slide images

et al. 2021

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Background and aims Visual assessment of mitotic figures in breast cancer (BC) remains a challenge. This is expected to be more pronounced in the digital pathology era. This study aims to refine the criteria of mitotic figure recognition, particularly in whole slide images (WSI). Method and results Haematoxylin and eosin (H&E)‐stained BC sections (n = 506) were examined using light microscopy (LM) and WSI. A set of features for identifying mitosis in WSI and to distinguish true figures from mimickers was developed. Changes in the mitotic count between the two platforms was explored. Morphological features of mitoses were recorded separately, including absence of nuclear membrane, chromatin hairy‐like projections, shape, cytoplasmic features, mitotic cell size and relationship to surrounding cells. Each mitotic phase has its own mimickers. Fifty‐eight per cent of mitoses showed absent hairy‐like projection in WSI; however, 89% retained their ragged nuclear border, which distinguished them from mimickers including apoptotic cells, lymphocytes and dark elongated hyperchromatic structures. Mitosis in WSI showed loss of fine details, and there was a 20% average reduction rate of mitotic counts when compared to the same area on LM. Using refined mitosis recognition criteria in WSI resulted in a twofold improvement of interobserver concordance. However, when compared to LM, 19% of cases were underscored in WSIs. Conclusions All morphological features of mitosis should be considered to enable recognition and differentiation from their mimickers, particularly in WSI, to ensure reliable BC grading. Refining mitotic cut‐offs per specific area when using WSI, based on the degree of reduction and association with outcome, is warranted.

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“…As the microscopic objectives used (numeric aperture, field diameter at specimen level) are difficult to control, mitoses per high power field may be quite variable; because of this, results cannot be compared between various laboratories. Therefore, counting per mm 2 reduced the need for additional standardisation and gave significant findings [11] (Table 3).…”

Section: Discussionmentioning

confidence: 99%

“…The higher sensitivity of crystal violet could be described due to its basic nature and its high affinity for highly acidic chromatin [9]. The overall higher diagnostic efficiency of crystal violet could also be explained due to the modification of a staining method by using 1N HCL at 60°C, therefore causing an increase in contrast due to the light staining of the cytoplasm, which is likely to be a result of the decreased RNA content following hydrolysis [11].…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Crystal Violet Stain to Study Mitotic Figures in Oral Epithelial Dysplasia

Sajjad¹,

Sajjad²,

Minhas³

et al. 2019

Open Access Maced J Med Sci

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AIM: To evaluate mitotic activity in the different grades of oral epithelial dysplasia using 1% crystal violet stain. MATERIAL AND METHODS: A descriptive study was conducted in the Department of Histopathology of the Post Graduate Medical Institute, Lahore on a total of thirty-three cases of the Oral Epithelial Dysplasia (OED). Fresh, frozen paraffin-embedded archival tissue blocks were collected from Lahore General Hospital, Lahore & Oral & Maxillofacial Surgery Department of Nawaz Sharif Hospital, Yakki Gate, Lahore. The representative sections were taken and, after processing, mounted on glass slides and stained with H&E and crystal violet stains. The stained slides were then examined under an optical microscope. The efficacy of 1% crystal violet stain to identify mitotic figures in the different grades of oral epithelial dysplasia was assessed with the sample t-test. A difference of p < 0.05 was considered to be significant. RESULTS: A comparison of the mitotic figure count in two categories in sections stained with both stains showed a statistically significant difference. An increase in the mean mitotic count was noted in the sections of OED stained with crystal violet in comparison to the sections of OED stained with H&E which was statistically significant (p = 0.00). CONCLUSION: Counting of mitotic cell is the rapid and simplest way of evaluating the proliferative activity of cells. Crystal violet stain can be a rationalised step in the staining of mitotic figures compared to the usual H&E staining and can be employed as a selective stain during routine histopathological procedures.

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Image analysis assisted study of mitotic figures in oral epithelial dysplasia and squamous cell carcinoma using differential stains

Cited by 12 publications

References 37 publications

Squamous Cell Carcinoma Developing De Novo in an Untreated, Nonsun-Exposed Psoriatic Plaque

Squamous Cell Carcinoma Developing De Novo in an Untreated, Nonsun-Exposed Psoriatic Plaque

Visual assessment of mitotic figures in breast cancer: a comparative study between light microscopy and whole slide images

Efficiency of Crystal Violet Stain to Study Mitotic Figures in Oral Epithelial Dysplasia

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