Image Processing and Cryo-Transmission Electron Microscopy; Example of Human Proteasome

Abstract

Cryo-transmission electron microscopy (cryo-TEM) allows us to perform structural analysis of a analyses of large protein complexes, which are difficult to analyze using X-ray crystallography or nuclear magnetic resonance. The most common examples of proteins used are ribosomes and proteasomes. In this paper, we briefly describe the advantage of cryo-TEM and the process of two-dimensional classification by considering a human proteasome as an example.

A microfluidic device for TEM sample preparation

“…Overall, the morphological observations are in line with reported data of similar samples prepared with conventional manual nsTEM. [21][22][23] The preparation of these two protein samples did not require further adjustments of the microfluidic device, hence demonstrating the versatility and robustness of the method.…”