Imaging CD8<sup>+</sup>T cells during diverse viral infections

Hickman, Heather D.

doi:10.1080/21659087.2015.1055425

Cited by 3 publications

(2 citation statements)

References 79 publications

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“…Intravital imaging of the CNS was performed using an adapted protocol 55 , 56 . In brief, mice were injected with intravascular dye (200 μl of 0.1% Evans blue) and 10 μg anti-Thy1.1 PE where noted prior to being anesthetized IP with avertin (400 μl/20 g).…”

Section: Methodsmentioning

confidence: 99%

Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

et al. 2020

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The central nervous system (CNS) is classically viewed as immune-privileged; however, recent advances highlight interactions between the peripheral immune system and CNS in controlling infections and tissue homeostasis. Tissue-resident memory (Trm) CD8 + T cells in the CNS are generated after brain infections, but it is unknown whether CNS infection is required to generate brain Trm cells. We show that peripheral infections generate antigen-specific CD8 + memory T cells in the brain that adopt a unique Trm signature. Upon depletion of circulating and perivascular memory T cells, this brain signature was enriched and the surveilling properties of brain Trm cells revealed by intravital imaging. Importantly, peripherally induced brain Trm cells showed evidence of rapid activation, enhanced cytokine production and mediated protection after brain infections. These data reveal that peripheral immunizations can generate brain Trm cells and will guide potential use of T cells as therapeutic strategies against CNS infections and neurological diseases.

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Section: Methodsmentioning

confidence: 99%

Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

et al. 2020

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“…The imbalance of Th17/Treg was reflected in the increased in IL-17 secreted by proinflammatory Th17 cells, while the content of Foxp3, CTLA4, PD-1, and galectin-1 secreted by Treg cells was decreased, which is consistent with our results. In addition, Tc cells, belonging to CD8+ T cells, play an crucial role in anti-tumor [36] and anti-virus behavior [37]. The cytotoxic effect of Tc cells can specifically kill those corresponding target cells.…”

Section: Discussionmentioning

confidence: 99%

The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows

Xiao

et al. 2020

Preprint

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As a common group-occurring disease, endometritis is caused by endometrial cell injury and chronic inflammation of uteri infected by pathogenic microorganisms, and leads to reproductive failure in dairy cows. Changes in the immune response function of the uteri affect the occurrence and development of endometritis. It was recently discovered that exosomes act as a functional regulator secreted by a variety of cells. However, its regulatory mechanism in the local immune response of the uteri is still unclear. In this study, flow cytometry was adopted to identify that the number of T lymphocytes in the uteri with endometritis increased while the compositional proportion also changed, namely that of Tc cell increased very significantly and that of Th and Treg cells decreased very significantly. Moreover, the functional marker factor Foxp3 of Treg cells was extremely significantly decreased, and the functional marker factor of Th17 cells was significantly increased. The mRNA expression of immune tolerance regulators PD1, CTLA4 and Galectin-1 in the group of Treg cells which were co-incubated with LPSstimulated endometrial epithelium-derived exosomes was very significantly lower than in the group of Treg cells which were co-incubated with normal EEC-derived exosomes. Furthermore, the protein expression of PD1, CTLA4, Galectin-1, Foxp3, and IL-17 was consistent with the results in the Treg cells co-incubated with different source exosomes in mice. Therefore, it was demonstrated that endometrial epithelium-derived exosomes act as a vital regulator of changes in the composition and function of T lymphocyte subsets in the uterus of dairy cows. Title page The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows Running head: Exosomes activate T Lymphocytes

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New insights into antiviral immunity gained through intravital imaging

Hickman

2017

Current Opinion in Virology

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Imaging CD8⁺T cells during diverse viral infections

Cited by 3 publications

References 79 publications

Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows

New insights into antiviral immunity gained through intravital imaging

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Imaging CD8+T cells during diverse viral infections

Cited by 3 publications

References 79 publications

Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows

New insights into antiviral immunity gained through intravital imaging

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Imaging CD8⁺T cells during diverse viral infections