Imaging mass spectrometry for accessing molecular changes during burn wound healing

Abstract

The spatiotemporal analysis of the proteomic profile during human wound healing is a critical investigative step that can establish the complex interplay of molecular events that comprise the local response to burn injury. Partial-thickness wound samples with adjacent "normal" skin were collected from twenty-one patients with burn wounds and examined across a time spectrum ranging from the acute injury period at 3, 6, 11 days to the later hypertrophic scar period at 7 and 15 months. The techniques used for histology-directed tissue analyses highlighted inflammatory protein markers at the early time points after injury with diminished expression as burn wounds progressed into the proliferative phase. The datasets show the usefulness of MALDI MS and imaging mass spectrometry as discovery approaches to identify and map the cutaneous molecular sequence that is activated in response to the unique systemic inflammatory response following burn trauma. This information has the potential to define the unique factors that predispose human burn victims to disfiguring hypertrophic scar formation.

Mass spectrometry imaging for clinical research – latest developments, applications, and current limitations

“…from the acute period (days after trauma) to the later hypertrophic scar (months after trauma). 239 This histology-directed tissue analysis found that the expression of several inflammatory proteins was correlated to the wound healing process, which could be used to predict whether human burn victims are at risk of disfiguring hypertrophic scar formation.…”

Sample Treatment for Tissue Proteomics in Cancer, Toxicology, and Forensics

“…Hart et al [59] utilised MALD-MSI for the study of ex vivo human skin in a search for biomarkers of skin sensitization and protocols for the preparation of skin for imaging have been published [65][66][67]. Wound healing has been the subject of a number of MSI publications [35,38,68,54,69]. Taking as illustrative of these the 2016 study of burn wounds by Taverna et al [54] the methodology used can be summarised as Table 7 shows and it is a typical methodology for intact protein imaging in skin.…”

“…Wound healing has been the subject of a number of MSI publications [35,38,68,54,69]. Taking as illustrative of these the 2016 study of burn wounds by Taverna et al [54] the methodology used can be summarised as Table 7 shows and it is a typical methodology for intact protein imaging in skin.…”

“…Table 7. Protocol for the preparation of human skin for direct protein analysis by MALDI-MSP and MALDI-MSI adapted from Taverna et al [54].…”

Metformin effects on head and neck squamous carcinoma microenvironment: Window of opportunity trial

“…Specimens were collected from the tumor and processed, as previously described. [33][34][35] Briefly, samples were wrapped in aluminum foil, snap-frozen in liquid nitrogen, and then stored at 280 o C.…”