Imaging Techniques for Clinical Burn Assessment with a Focus on Multispectral Imaging

Thatcher, Jeffrey E.; Squiers, John J.; Kanick, Stephen C.; King, Darlene R.; Yang, Gongping; Wang, Yulin; Mohan, Rachit; Sellke, Eric W.; DiMaio, J. Michael

doi:10.1089/wound.2015.0684

Cited by 73 publications

(62 citation statements)

References 101 publications

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“…Most light sources for PDT seek to utilize the 630 nm red absorption peak, thus improving tissue penetration. Unlike pulsed‐dye laser or potassium titanyl phosphate laser, the 635 nm laser will not be absorbed by oxyhemoglobin, given that the absorption coefficient of oxyhemoglobin is very low at 635 nm . As the laser energy in PDT is usually around 100 mW/cm 2 , the thermal effect of PDT lasers on laryngeal mucosa is negligible .…”

Section: Discussionmentioning

confidence: 99%

Photodynamic therapy induces antifibrotic alterations in primary human vocal fold fibroblasts

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Photodynamic therapy induces antifibrotic alterations in primary human vocal fold fibroblasts

et al. 2018

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“…Currently, it is being used in the operating room in real‐time as a device known as SPY Elite (Novadaq, Burnaby, Vancouver, Canada), but it is invasive and expensive for general use . For those interested in more detail regarding various technologies, a comprehensive review is available …”

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confidence: 99%

“…The reflectance spectral patterns in visible and infrared wavelengths of light can then be used to classify damage to tissue, such as in burn wounds and the surrounding tissue. When spectral data are compared to a reference library of data, the process of pattern recognition can be employed for computer‐aided diagnosis . Ongoing studies of MSI and machine learning are using burn reflectance spectra and histological assessment from different burn thicknesses to build a reference database.…”

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confidence: 99%

“…Ongoing studies of MSI and machine learning are using burn reflectance spectra and histological assessment from different burn thicknesses to build a reference database. The histological assessment (H&E) is used as “ground truth” reference material (“gold standard” or “criterion standard”) for building this classification algorithm . In this issue of the Journal of Cutaneous Pathology , Dr. Gibson shows that LDH staining is reliable and reproducible.…”

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confidence: 99%

“…7 For those interested in more detail regarding various technologies, a comprehensive review is available. 9 Although histology is not practical for real-time clinical assessment, it can be a useful tool for research. In the experimental mammalian-model setting where burns can be created using controlled temperatures and contact times, the corresponding histology has been used to characterize changes to determine the depth of injury.…”

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confidence: 99%

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Reconsidering the H&E stain as the gold standard in assessing the depth of burn wounds

Rosenberg

2017

J Cutan Pathol

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While histological examination is considered by most as the gold standard for burn depth assessment, it has no practical use in the clinical setting. It has, however, been used in the research setting, as a mean for evaluating emerging techniques of depth measurement. Due to the limitations of the H&E stain, other stains have also been explored, such as lactate dehydrogenase (LDH), as presented in this issue, in "Improving the Histologic Characterization of Burn Depth." As the determination of burn depth is not a typical subject in dermatopathology, a summary of selected techniques and the possible role for the LDH stain in future research, is described herein.

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Quantitative and Qualitative Evaluation of Supercontinuum Laser‐Induced Cutaneous Thermal Injuries and Their Repair With OCT Images

Fan

Xin

et al. 2020

Lasers Surg Med

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Background and Objectives Laser‐induced thermal injuries are a heavily researched topic in laser medicine and biomedicine. To explore the quantitative biological effects of laser‐induced injury repair, we propose an optical imaging‐based in vivo evaluation method and discuss the injury effect and repair characteristics of skin tissue. Study Design/Materials and Methods A supercontinumm laser (spectrum width ranged from 400 to 2400 nm) was used to irradiate live mice. The radiation doses were set as 32.85, 65.69, 98.4, and 197.08 J/cm2. Laser‐induced cutaneous thermal injuries were observed and measured after laser radiation. Optical coherence tomography (OCT) was used to image the injured spots. The optical attenuation coefficient (OAC) was computed using a Fourier‐domain algorithm. Three‐dimensional (3D) visualizations of the injured spots in the OCT images were constructed to qualitatively demonstrate the degree of the thermal injury at different times. The corresponding pathological changes of the skin injuries were observed dynamically. Results Under increasing radiation doses, the injured spots, which ranged in degree from mild to severe, appeared accordingly. The mean OAC value of normal skin was 1.15 ± 0.01 mm−1. The mean values of the OAC maps in the region of interests (ROIs) were 0.88 ± 0.05, 0.91 ± 0.02, 0.93 ± 0.04, and 0.98 ± 0.08 mm−1 at each of the four radiation levels, respectively. Additionally, the 3D OCT visualization showed a directed view of the injured spots and the healing process and was used as a qualitative evaluation method. The OCT images and pathological results showed that the damage tended to vary across all groups, but this trend tended to weaken as the radiation dose increased. Conclusion Optical imaging provides an in vivo noninvasive and real‐time evaluation method to comprehensively quantify supercontinuum laser‐induced thermal injuries and wound healing. The OACs and 3D visualizations of the OCT cubic data enable the areas of the direct injured spots to be measured and the inner structure to be viewed. The results obtained with these techniques indicate that the skin injuries are aggravated and tissue repair trends weaken as the radiation doses increase. This method would have a potential application for laser‐medicine and laser‐biological effects in the future. Lasers Surg. Med. © 2020 Wiley Periodicals LLC

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Imaging Techniques for Clinical Burn Assessment with a Focus on Multispectral Imaging

Cited by 73 publications

References 101 publications

Photodynamic therapy induces antifibrotic alterations in primary human vocal fold fibroblasts

Photodynamic therapy induces antifibrotic alterations in primary human vocal fold fibroblasts

Reconsidering the H&E stain as the gold standard in assessing the depth of burn wounds

Quantitative and Qualitative Evaluation of Supercontinuum Laser‐Induced Cutaneous Thermal Injuries and Their Repair With OCT Images

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