Imaging with Coherent X-rays: From the Early Synchrotron Tests to SYNAPSE

Margaritondo, G.; Hwu, Y.

doi:10.3390/jimaging7080132

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…This technique relies on the detection of changes in the phase of a coherent X-ray beam caused by slight differences in the thickness and refractive index of tissues to enhance the contrast at the edges of microstructural boundaries ( 25 – 27 ). The best source of coherent X-rays are synchrotron light sources ( 28 ), which additionally provide advantages over conventional µCT systems by reducing imaging times for an improved scan quality (with reduced to no beam hardening) and higher spatial resolution ( 29 , 30 ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced contrast synchrotron X-ray microtomography for describing skeleton-associated soft tissue defects in zebrafish mutants

et al. 2023

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Detailed histological analyses are desirable for zebrafish mutants that are models for human skeletal diseases, but traditional histological techniques are limited to two-dimensional thin sections with orientations highly dependent on careful sample preparation. On the other hand, techniques that provide three-dimensional (3D) datasets including µCT scanning are typically limited to visualizing the bony skeleton and lack histological resolution. We combined diffusible iodine-based contrast enhancement (DICE) and propagation phase-contrast synchrotron radiation micro-computed tomography (PPC-SRµCT) to image late larval and juvenile zebrafish, obtaining high-quality 3D virtual histology datasets of the mineralized skeleton and surrounding soft tissues. To demonstrate this technique, we used virtual histological thin sections and 3D segmentation to qualitatively and quantitatively compare wild-type zebrafish and nkx3.2-/- mutants to characterize novel soft-tissue phenotypes in the muscles and tendons of the jaw and ligaments of the Weberian apparatus, as well as the sinus perilymphaticus associated with the inner ear. We could observe disrupted fiber organization and tendons of the adductor mandibulae and protractor hyoideus muscles associated with the jaws, and show that despite this, the overall muscle volumes appeared unaffected. Ligaments associated with the malformed Weberian ossicles were mostly absent in nkx3.2-/- mutants, and the sinus perilymphaticus was severely constricted or absent as a result of the fused exoccipital and basioccipital elements. These soft-tissue phenotypes have implications for the physiology of nkx3.2-/- zebrafish, and demonstrate the promise of DICE-PPC-SRµCT for histopathological investigations of bone-associated soft tissues in small-fish skeletal disease models and developmental studies more broadly.

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Section: Introductionmentioning

confidence: 99%

Enhanced contrast synchrotron X-ray microtomography for describing skeleton-associated soft tissue defects in zebrafish mutants

et al. 2023

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“…An alternative approach is computer simulations of experimental images. We refer to several papers dealing with different pore models [11][12][13] and reviews of recent literature [14,15].…”

Section: Introductionmentioning

confidence: 99%

Problems with Evaluation of Micro-Pore Size in Silicon Carbide Using Synchrotron X-ray Phase Contrast Imaging

Argunova

Kohn

2022

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We report near- and far-field computer simulations of synchrotron X-ray phase-contrast images using a micropipe in a SiC crystal as a model system. Experimental images illustrate the theoretical results. The properties of nearly perfect single crystals of silicon carbide are strongly affected by μm-sized pores even if their distribution in a crystal bulk is sparse. A non-destructive technique to reveal the pores is in-line phase-contrast imaging with synchrotron radiation. A quantitative approach to evaluating pore sizes is the use of computer simulations of phase-contrast images. It was found that near-field phase-contrast images are formed at very short distances behind a sample. We estimated these distances for tiny pores. The Fresnel zones did not provide any information on the pore size in the far-field, but a contrast value within the first Fresnel zone could be used for simulations. Finally, general problems in evaluating a micro-pore size via image analysis are discussed.

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“…Interestingly, four review papers illustrate the principles and perspective of radiographic imaging with muons [ 20 ], an overview of photon-counting spectral imaging detectors [ 21 ], a comparison of imaging techniques for biological and biomedical studies [ 22 ] and a review of the use of coherent X-rays, from early synchrotron tests to the most recent brain studies [ 23 ].…”

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confidence: 99%