Imidazolium-based ionic liquid-type surfactant as pseudostationary phase in micellar electrokinetic chromatography of highly hydrophilic urinary nucleosides

Effect of ionic liquids on the interaction between liposomes and common wastewater pollutants investigated by capillary electrophoresis

“…These experiments were performed by adding ILs to the BGE solution containing liposomes. Imidazolium ILs and other nitrogen-based ILs, linear and cylic, have previously been successfully used as BGE additives in the separation of various compounds [24,[34][35][36][37][38][39][40][41] and as surfactant modifiers [42][43][44] in EKC. Phosphonium ILs have not been as widely employed as pseudostationary phases in EKC, however, our group has successfully separated neutral compounds [23], aromatic compounds, and steroids [20] by using long alkyl chained phosphonium ILs.…”

A novel method of liquid chromatography–tandem mass spectrometry combined with chemical derivatization for the determination of ribonucleosides in urine

“…The early used methods included high performance liquid chromatography (HPLC) coupled with UV detector [11] and enzyme-linked immunosorbent assay (ELISA) [12]. The currently favored methods are chromatography or capillary electrophoresis coupled with mass spectrometry (MS) [13][14][15]. Although MS is more specific and sensitive than UV detector, the extraction and enrichment methods are still necessary for the analysis of ribonucleosides due to the complex components of biological samples and low abundance of ribonucleosides.…”

Boronate affinity‐assisted MEKC separation of highly hydrophilic urinary nucleosides using imidazolium‐based ionic liquid type surfactant as pseudostationary phase

“…We have studied the mechanism of interaction between the micelles formed by C 14 MImBr and the negatively charged nucleosides. It was confirmed that electrostatic interaction is the main mode of interaction, and hydrophobic interaction can be considered to be negligible . We have demonstrated that using only 20 mmol/L C 14 MImBr in the BGE (5 mmol/L tetraborate, pH 9.38), a complete separation of all the studied analytes (existing as negatively charged complexes) is achieved.…”

“…These methods employ either RP‐HPLC, HILIC (due to their highly hydrophilic nature) or MEKC for the separation of similar compounds. Although separation by HILIC might be seen as the state of art for the determination of these highly hydrophilic nucleosides, we have demonstrated that our developed MEKC strategy provides a faster, more efficient, highly reproducible and highly selective method for the analysis of these hydrophilic metabolites . Most of the reported MEKC methods for the analysis of nucleosides are based on using SDS as pseudostationary phase (PSP) for the separation of these compounds as neutral species, however, due to their high hydrophilicity, a high concentration of the PSP (up to 300 mmol/L) is needed to obtain resolution.…”