Iminocoumarin-based fluorescence probe for intracellular H2O2 detection with a red emission and a large Stokes shift

Small-molecule fluorescent probes based on covalent assembly strategy for chemoselective bioimaging

“…16 ). 91 During the detection process, Probe 1 was non-fluorescent initially. Once treated with H 2 O 2 , H 2 O 2 caused the cleavage of the boronic ester moiety and the quinone derivative would leave quickly, subsequently resulting in an interference ability in aqueous media, which can also be applied to detect intracellular H 2 O 2 in HeLa cells.…”

A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging

“…The structure of CMB was accurately validated using the 1 H NMR and HRMS analyses presented in the Supporting Information (Supplementary Figures S1-S4). Herein, a smart small-molecule fluorescent probe, CMB, was designed and synthesized for H2O2 imaging in solution, cells, and zebrafish by employing coumarin as fluorophore and the boronate as the recognition group [28][29][30] (Scheme 1). A short alkyl chain is used to connect the morpholine group with coumarin to improve the biological applicability of the probe.…”

“…Probe CMB can not only shows enhanced fluorescence signal to H 2 O 2 with good selectivity, but also exhibits desirable imaging effects on H 2 O 2 in cells and zebrafish, which indicates that CMB has the potential to monitor the content and level of hydrogen peroxide in biological systems. Herein, a smart small-molecule fluorescent probe, CMB, was designed and synthesized for H2O2 imaging in solution, cells, and zebrafish by employing coumarin as fluorophore and the boronate as the recognition group [28][29][30] (Scheme 1). A short alkyl chain is used to connect the morpholine group with coumarin to improve the biological applicability of the probe.…”

Research progress in the development of organic small molecule fluorescent probes for detecting H2O2

“…In addition to the description above, there were many probes designed using this technique. [60][61][62][63][64][65][66][67][68][69][70] The photo-damage and photo-bleaching properties of single photon imaging technology have prevented longterm imaging during bioimaging process, and it has strong background interference and low UV transmittance, which limits their applications in deep tissue imaging. By contrast, two-photon (TP) uorescent probes can greatly overcome these defects.…”