Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Abdullah, Rohana; Zainal, A.; Heng, Wee Yew; Li, Leaw Chui; Beng, Yeap Chee; Phing, Lee Mei; Sirajuddin, Salwa Abdullah; Ping, Winnie Yap Soo; Joseph, Juanita Lourdes; Jusoh, Siti Azma; Muad, Muhammad Rashdan; Huey, Yeun Li

doi:10.2225/vol8-issue1-fulltext-1

Cited by 36 publications

(28 citation statements)

References 11 publications

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“…Coconut is thus apparently more tolerant to the effect of kanamycin than the species mentioned above. Somehow contrasting, in the case of another palm species E. guineensis, the minimum concentration needed was above 200 mg/L kanamycin (Abdullah et al 2005). Based on the present results, we decided that the concentration of 400 mg/L kanamycin in semisolid Y3 medium without AC was adequate for selection of the coconut transformed cells.…”

Section: Kanamycin For Transformed Plant Cells Selectionmentioning

confidence: 67%

“…The gene gusA encoding GUS is frequently used for this and the corresponding development of blue staining (Parveez & Christou 1998;Ramli & Abdullah 2003;Abdullah et al 2005;Solís-Ramos et al 2010a).…”

Section: Resultsmentioning

confidence: 99%

“…Parveez and Christou (1998) reported the regeneration of plants from oil palm embryogenic callus transformed by biobalistics. Abdullah et al (2005) developed a method for genetic transformation using immature zygotic embryos of oil palm, and through analysis of β-glucuronidase (GUS) expression, they identified 97.4% of transformation frequency by biolistics and 64.4% through Agrobacterium tumefaciens co-culture. No studies have been reported on defence mechanisms or related genes for coconut.…”

Section: Introductionmentioning

confidence: 99%

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Transient genetic transformation of embryogenic callus of Cocos nucifera

et al. 2011

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Coconut palm (Cocos nucifera) is a plant species recalcitrant to in vitro morphogenesis and no protocols for the genetic transformation of coconut tissues have been published. The present study aimed to develop a protocol for genetic transformation of this palm species; evaluating reporter genes, transformation methods, and conditions for the use of antibiotics to select transformed plant cells. The gene gusA was first used for Agrobacterium tumefaciens mediated transformation of coconut embryogenic calli. However, endogenous GUS-like activity was found in calli not co-cultured with bacteria. Then essays for Agrobacterium-mediated transformation were developed using green and red fluorescent genes. Both genes are suitable as reporter genes for coconut transformation. In order to establish a protocol for coconut genetic transformation, an approach was used that combined biobalistics to generate micro-wounds in explants, vacuum infiltration and co-culture with Agrobacterium tumefaciens (C58C1 + pER10W-35SRed containing the embryogenesis related gene WUSCHEL). Calli treated with the combined protocol showed red fluorescence with greater intensity and greater area than calli treated with either biobalistics or infiltration, followed by bacteria co-culture. PCR amplification of DNA extracts from transformed embryogenic callus produced a band with the expected size using WUSCHEL primers (862 bp). No band was obtained using the VirE2 primers. This is the first report of transient genetic transformation of C. nucifera and it is the first step toward a protocol that will be useful for the study of the role of genes of interest and for practical applications, such as the improvement of coconut micropropagation via somatic embryogenesis.

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Section: Kanamycin For Transformed Plant Cells Selectionmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transient genetic transformation of embryogenic callus of Cocos nucifera

et al. 2011

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“…On the basis of culture, MS is widely used as a basal medium for proliferation in oil palm tissue culture [12][13][14] and germination tests 15 . Culture media play a significant role in germination.…”

Section: Discussionmentioning

confidence: 99%

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Thawaro¹,

Te-chato²

2010

ScienceAsia

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Nutritional requirements for germination of mature zygotic embryos of four genotypes of Elaeis guineensis Jacq. Dura × Pisifera were assessed. A factorial experiment was conducted with four genotypes from separate crossing events and three culture media to show Interactions between genotypes and culture media on seedling germination (p < 0.05). The highest frequency of normal seedling germination was facilitated by 1/2 Murashige and Skoog (MS) medium (29.2%), followed by MS (15.0%) and Blaydes (12.9%). Speed of shoot formation followed a similar trend. Roots were induced most rapidly in 1/2 MS medium, followed by Blaydes and then MS. Root induction was similar in 1/2 MS medium (2.29%) and Blaydes medium (1.25%) but only significantly higher than in MS (0.00%).

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“…Current conventional breeding programmes for oil palm are supported by modern biotechnology approaches such as the use of tissue culture, genetic transformation and marker-aided selection, to further improve oil palm commercial value and productivity (Mayes et al 2000). Over the past 10 years, our group has been actively involved in genetic engineering of oil palm (Abdullah et al 2003;Abdullah et al 2005). Although many studies have been done, but there are very few publications on genetic manipulation of oil palm, probably due to the high commercial implication tagged to the crop and also due to the crops own physiological characteristics such as long generation cycle.…”

mentioning

confidence: 99%

Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

Lee¹,

Yeun²,

Abdullah³

2006

Electron. J. Biotechnol.

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Oil palm, like all other crops, is susceptible to attack from several insect pests causing significant reduction in productivity. In the past, cry genes from Bacillus thuringiensis have been reported to be effective in conferring resistance towards insect pests in crops such as corn and rice. One of the advantages of these toxin proteins is their specificity towards certain harmful insects. A rapid and efficient method was developed for the transformation and evaluation of CryIA(b) expression in oil palm. A recombinant vector was introduced into immature embryos (IEs) of oil palm via the biolistic method. More than 700 putative transformed IEs from independent transformation events were generated. Transient transformation efficiency of 81-100 % was achieved. We found that pretreatment of target tissues with phytohormones is essential for increasing the transformation efficiency. This finding could enable higher transformation rate in

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Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Cited by 36 publications

References 11 publications

Transient genetic transformation of embryogenic callus of Cocos nucifera

Transient genetic transformation of embryogenic callus of Cocos nucifera

Untitled

Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

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