Immediate early response protein 2 regulates hepatocellular carcinoma cell adhesion and motility via integrin β1-mediated signaling pathway

Xu, Zhengxin; Zhu, Lei; Wu, Wenjuan; Liao, Yuexia; Zhang, Weicheng; Deng, Zijing; Shen, Jingyuan; Yuan, Qing; Shen, Weishou

doi:10.3892/or.2016.5215

Cited by 18 publications

(27 citation statements)

References 33 publications

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“…Xu et al [26] reported that IER2 promotes the migration and invasion of hepatocellular carcinoma cell adhesion and motility. Xu et al [27] demonstrated that IER2 promotes the migration and invasion of hepatocellular carcinoma cells via regulating the activity of Rho GTPases. Moreover, Moriya et al [28] reported that ier2 mRNA was distributed in the telencephalon, midbrain and the hypothalamus.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of Some Novel Potential Biomarkers Associated With Osteoarthritis and Nervous System

Guo¹,

Qin²,

Chen³

et al. 2021

Preprint

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BackgroundOsteoarthritis (OA) is an age-related chronic inflammatory and degenerative changes that carries heavy burden for individuals and the society. The specific mechanism of OA still remains unclear today, which requires new methods and technologies to achieve some new breakthrough. Bioinformatics technology is a novel method to extract genetic information from many diseases. In this study, we aims at screening out some key genes to help to illuminate the pathogenesis of OA to help to diagnosis and cure it.Objective and MethodsBioinformatics technology was used to screen some key target genes that were closely related to OA and nervous system, and by using qRT-PCR to preliminary verify the results.ResultsIn this work, we analysis three gene expression profiles, GSE114007, GSE51588, and GSE55457, that downloaded from the Gene Expression Omnibus database (GEO). At last, a total of 878 DEGs were identified with dataset GSE114007 (P<0.05 and |logFC|>1.5), consisting of 495 up-regulated genes and 383 down-regulated genes between the osteoarthritis and normal cartilage tissues. And by combining with the screened results of GSE51588 and GSE55457, finally, three genes, HES1, JUN, and IRE2, which were closely correlated with the nervous system that may help to diagnosis and cure osteoarthritis in the future were identified, and the result of qRT-PCR preliminary confirmed our finding.ConclusionHES1, JUN, and IRE2 were three potential genes related to osteoarthritis and nervous system that may help to diagnosis and cure OA.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of Some Novel Potential Biomarkers Associated With Osteoarthritis and Nervous System

Guo¹,

Qin²,

Chen³

et al. 2021

Preprint

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“…These networks highlighted the interconnectivity of genes that were differentially expressed at different stages of tendon healing and provided insight into the importance of these genes. For instance, integrins (ITGs) are transmembrane receptors that facilitate cell-ECM adhesion and have two main functions: attach the cell to the ECM and transduce signals from the ECM to the cell [43,44]. The biological functions of ITGs family are mainly associated with adhesion.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of digital flexor tendons after injury in a chicken model

Mao

Chen

et al. 2020

Bioscience Reports

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Background: Modulation of tendon healing remains a challenge because of our limited understanding of the tendon repair process. Therefore, we performed the present study to provide a global perspective of the gene expression profiles of tendons after injury and identify the molecular signals driving the tendon repair process. Results: The gene expression profiles of flexor digitorum profundus tendons in a chicken model were assayed on day 3, weeks 1, 2, 4, and 6 after injury using the Affymetrix microarray system. Principal component analysis (PCA) and hierarchical cluster analysis of the differentially expressed genes showed three distinct clusters corresponding to different phases of the tendon healing period. Gene ontology (GO) analysis identified regulation of cell proliferation and cell adhesion as the most enriched biological processes. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed that the cytokine–cytokine receptor interaction and extracellular matrix (ECM)–receptor interaction pathways were the most impacted. Weighted gene co-expression network analysis (WGCNA) demonstrated four distinct patterns of gene expressions during tendon healing. Cell adhesion and ECM activities were mainly associated with genes with drastic increase in expression 6 weeks after injury. The protein–protein interaction (PPI) networks were constructed to identify the key signaling pathways and hub genes involved. Conclusions: The comprehensive analysis of the biological functions and interactions of the genes differentially expressed during tendon healing provides a valuable resource to understand the molecular mechanisms underlying tendon healing and to predict regulatory targets for the genetic engineering of tendon repair. Tendon healing, Adhesion, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, Weighted Gene Co-expression Network Analysis, Protein–protein Interaction

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“…aureus . Ier2 is an immediate early response gene, affecting cell adhesion and motility [52, 53]. Although it has some DNA binding ability [54], nothing is known regarding its involvement in immunity or host defense.…”

Section: Discussionmentioning

confidence: 99%

Candidate genes on murine chromosome 8 are associated with susceptibility to Staphylococcus aureus infection in mice and are involved with Staphylococcus aureus septicemia in humans

et al. 2017

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We previously showed that chromosome 8 of A/J mice was associated with susceptibility to S. aureus infection. However, the specific genes responsible for this susceptibility are unknown. Chromosome substitution strain 8 (CSS8) mice, which have chromosome 8 from A/J but an otherwise C57BL/6J genome, were used to identify the genetic determinants of susceptibility to S. aureus on chromosome 8. Quantitative trait loci (QTL) mapping of S. aureus-infected N2 backcross mice (F1 [C8A] × C57BL/6J) identified a locus 83180780–88103009 (GRCm38/mm10) on A/J chromosome 8 that was linked to S. aureus susceptibility. All genes on the QTL (n~ 102) were further analyzed by three different strategies: 1) different expression in susceptible (A/J) and resistant (C57BL/6J) mice only in response to S. aureus, 2) consistently different expression in both uninfected and infected states between the two strains, and 3) damaging non-synonymous SNPs in either strain. Eleven candidate genes from the QTL region were significantly differently expressed in patients with S. aureus infection vs healthy human subjects. Four of these 11 genes also exhibited significantly different expression in S. aureus-challenged human neutrophils: Ier2, Crif1, Cd97 and Lyl1. CD97 ligand binding was evaluated within peritoneal neutrophils from A/J and C57BL/6J. CD97 from A/J had stronger CD55 but weaker integrin α5β1 ligand binding as compared with C57BL/6J. Because CD55/CD97 binding regulates immune cell activation and cytokine production, and integrin α5β1 is a membrane receptor for fibronectin, which is also bound by S. aureus, strain-specific differences could contribute to susceptibility to S. aureus. Down-regulation of Crif1 with siRNA was associated with increased host cell apoptosis among both naïve and S. aureus-infected bone marrow-derived macrophages. Specific genes in A/J chromosome 8, including Cd97 and Crif1, may play important roles in host defense against S. aureus.

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Immediate early response protein 2 regulates hepatocellular carcinoma cell adhesion and motility via integrin β1-mediated signaling pathway

Cited by 18 publications

References 33 publications

Bioinformatics Analysis of Some Novel Potential Biomarkers Associated With Osteoarthritis and Nervous System

Bioinformatics Analysis of Some Novel Potential Biomarkers Associated With Osteoarthritis and Nervous System

Transcriptome profiling of digital flexor tendons after injury in a chicken model

Candidate genes on murine chromosome 8 are associated with susceptibility to Staphylococcus aureus infection in mice and are involved with Staphylococcus aureus septicemia in humans

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