Immobilization of ascorbic acid oxidase

Bradberry, Charles W.; Borchardt, Ronald T.; Decedue, Charles J.

doi:10.1016/0014-5793(82)80950-2

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1982

1994

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Cited by 7 publications

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“…All reagents for buffer solutions were reagent grade. AAO was obtained from Boehringer-Mannheim (Indianapolis, IN) and immobilized as previously reported (8). AA standard solutions were prepared by diluting stock (1-10 mM AA in 0.10 M HC104) solutions with the buffer used as the carrier stream.…”

Section: Methodsmentioning

confidence: 99%

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Flow injection analysis with an enzyme reactor bed for determination of ascorbic acid in brain tissue

Bradberry¹,

Adams²

1983

Anal. Chem.

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Section: Methodsmentioning

confidence: 99%

“…AAO in soluble form has been employed previously for plant tissue assays (6). In addition, AAO has been immobilized on a Clark oxygen electrode (7), and its successful immobilization on Sepharose was recently reported (8).…”

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confidence: 99%