Immobilization of lipase Eversa Transform 2.0 on poly(urea–urethane) nanoparticles obtained using a biopolyol from enzymatic glycerolysis

Bresolin, Daniela; Hawerroth, Beatriz; Romera, Cristian de Oliveira; Sayer, Cláudia; Araújo, Pedro Henrique Hermes de; Oliveira, Débora de

doi:10.1007/s00449-020-02324-6

Cited by 18 publications

(7 citation statements)

References 49 publications

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“…The number of LVs were optimized using cross validation. The results clearly reveal that selected fatty acid features are predicted well and within the accuracies of published results [ 15 ]. The only calibration models that did not perform well in the conservative test set validation regime chosen, were the CLA and PUFA models developed, as shown in Supplementary Table S1 .…”

Section: Resultssupporting

confidence: 80%

“…Information on groups of fatty acids and even main single fatty acids can also be available from the spectra [ 13 , 14 ]. Studies also indicate use of FTIR in analysis of features such as protein composition, milk coagulation properties, acidity, and milk minerals [ 12 , 15 ]. Considerable efforts have also been put into the development of FTIR spectroscopy for herd management applications such as EB predictions and cow health monitoring.…”

Section: Introductionmentioning

confidence: 99%

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Exploring Dry-Film FTIR Spectroscopy to Characterize Milk Composition and Subclinical Ketosis throughout a Cow’s Lactation

Rachah

Reksen

Tafintseva

et al. 2021

Foods

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The use of technologies for measurements of health parameters of individual cows may ensure early detection of diseases and maximization of individual cow and herd potential. In the present study, dry-film Fourier transform infrared spectroscopy (FTIR) was evaluated for the purpose of detecting and quantifying milk components during cows’ lactation. This was done in order to investigate if these systematic changes can be used to identify cows experiencing subclinical ketosis. The data included 2329 milk samples from 61 Norwegian Red dairy cows collected during the first 100 days in milk (DIM). The resulting FTIR spectra were used for explorative analyses of the milk composition. Principal component analysis (PCA) was used to search for systematic changes in the milk during the lactation. Partial least squares regression (PLSR) was used to predict the fatty acid (FA) composition of all milk samples and the models obtained were used to evaluate systematic changes in the predicted FA composition during the lactation. The results reveal that systematic changes related to both gross milk composition and fatty acid features can be seen throughout lactation. Differences in the predicted FA composition between cows with subclinical ketosis and normal cows, in particular C14:0 and C18:1cis9, showed that dietary energy deficits may be detected by deviations in distinct fatty acid features.

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Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Exploring Dry-Film FTIR Spectroscopy to Characterize Milk Composition and Subclinical Ketosis throughout a Cow’s Lactation

Rachah

Reksen

Tafintseva

et al. 2021

Foods

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“…In a last paper from this group, a cyclic strategy was applied. Eversa in its free form was used in the glycerolysis of castor oil and the biopolyol obtained was used to produce a matrix where the enzyme was later immobilized [331].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

“…In a similar study, Eversa (referred as NS-40116) immobilized on nonporous polystyrene particles was used as biocatalyst in the hydrolysis of soybean oil, showing good operational stability after five consecutive cycles of hydrolysis [329]. Moreover Eversa® Transform 2.0 was used in glycerolysis of castor oil for the production of a biopolyol, which was used as a source of hydroxyl groups and stabilizer in the synthesis of poly(urea-urethane) nanoparticles [331].…”

Section: Other Applicationsmentioning

confidence: 99%

Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?

et al. 2021

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“… 14 This finding is also supported by Bresolin et al . 15 who found that immobilized CALB had ten‐fold lower enzyme hydrolytic activity compared to the immobilized ET 2.0.…”

Section: Resultsmentioning

confidence: 99%

Towards an alcohol‐free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases

et al. 2022

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BACKGROUND Crude palm oil (CPO) is rich with phytonutrients such as carotenoids and tocols which possesses many health benefits. The aim of this research was to develop a methanol‐free process to produce palm phytonutrients via enzymatic hydrolysis. In this work, triacylglycerol was hydrolyzed into free fatty acids (FFAs) using three different types of liquid lipases derived from Aspergillus oryzae (ET 2.0), Aspergillus niger (Habio) and Candida antartica (CALB). RESULTS ET 2.0 was found to be the best enzyme for hydrolysis. Under the optimum condition, the FFA content achievable was 790 g kg−1 after 24 h of reaction with 1:1 water‐to‐oil mass ratio at 50 °C and stirring speed of 9 × g. Furthermore, with the addition of 2 g kg−1 ascorbic acid, it was found that 98% of carotenoids and 96% of tocols could be retained after hydrolysis. CONCLUSION This work shows that enzymatic hydrolysis, which is inherently safer, cleaner and sustainable is feasible to replace the conventional methanolysis for the production of palm phytonutrients. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Immobilization of lipase Eversa Transform 2.0 on poly(urea–urethane) nanoparticles obtained using a biopolyol from enzymatic glycerolysis

Cited by 18 publications

References 49 publications

Exploring Dry-Film FTIR Spectroscopy to Characterize Milk Composition and Subclinical Ketosis throughout a Cow’s Lactation

Exploring Dry-Film FTIR Spectroscopy to Characterize Milk Composition and Subclinical Ketosis throughout a Cow’s Lactation

Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?

Towards an alcohol‐free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases

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