Immobilization technique for enhanced production of the immunosuppressant mycophenolic acid by ultraviolet and gamma-irradiated <i>Penicillium roqueforti</i>

Ismaiel, Ahmed A.; Ahmed, Ashraf S.; Elsayed, Ebrahim

doi:10.1111/jam.12828

Cited by 24 publications

(13 citation statements)

References 46 publications

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“…The DLS analysis of the aqueous dispersion of the prepared Cur-NLs revealed the formation of nanoparticles with an average diameter of 72 26 ± 20 nm and the electron micrographs showed spherical shape. The particle size and shape reported herein were in agreement with those reported earlier using different preparation techniques [31][32][33]. The hyperbolic relation between the particle size and the surface specific dissolution rate increases the solubility due to the larger surface area which promotes dissolution [34].…”

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Nanoencapsulation of Curcumin and Its Protective Effects against CCl₄-Induced Hepatotoxicity in Mice

Niu

Liu

et al. 2019

Journal of Nanomaterials

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Curcumin is a natural phenolic compound extracted from the herb Curcuma longa L. rhizome and has received much attention on account of its biological properties. However, its poor solubility and low bioavailability limit its use. The purpose of this study was to synthesize curcumin-loaded nanoliposomes (Cur-NLs) to improve their bioavailability and evaluate the hepatoprotective effect of Cur-NLs against tetrachloromethane- (CCl4-) induced acute liver injury in mice. We prepared Cur-NLs by thin film dispersion method, and the characterizations of Cur-NLs were measured by transmission microscope, laser particle size analyzer, infrared spectrometer, and X-ray diffraction. After 14 days pretreatment of Cur-NLs, free curcumin, silybin, or PBS, the models of acute liver injury were established by CCl4 intraperitoneal injection in mice. The organ index, biochemical liver function parameters, histopathology, and antioxidant enzyme activities of liver tissues were measured further to evaluate the protective effects of Cur-NLs on liver injury. Compared with the CCl4 model control group, pretreatment of Cur-NLs effectively reduced the serum levels of ALT, AST, and ALP and attenuated the hepatic necrosis induced by CCl4 intoxication. Furthermore, Cur-NL pretreatment remarkably exhibited decreased MDA level and increased SOD, GPx, and CAT activities compared to CCl4 model control group. Compared with the free curcumin group, the Cur-NLs also showed a better hepatoprotective effect. These observations imply that Cur-NLs act as a promising hepatoprotective agent in reducing liver oxidative stress produced by different stress factors.

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confidence: 90%

Nanoencapsulation of Curcumin and Its Protective Effects against CCl₄-Induced Hepatotoxicity in Mice

Niu

Liu

et al. 2019

Journal of Nanomaterials

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“…In partial accordance with these results, production of the anticancer drug paclitaxel by A. fumigatus and A. tenuissima irradiated at 750 Gy was twice as compared by the non‐irradiated controls (El‐Sayed et al , ). Moreover, irradiation of two Penicillium roqueforti strains at the same dose (750 Gy) significantly enhanced the production of the immunosuppressant of mycophenolic acid (Ismaiel et al , ; El‐Sayed et al ).…”

Section: Discussionmentioning

confidence: 99%

A novel source of the cardiac glycoside digoxin from the endophytic fungus Epicoccum nigrum : isolation, characterization, production enhancement by gamma irradiation mutagenesis and anticancer activity evaluation

2019

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Aims Different endophytic fungi were isolated and screened for their digoxin‐producing ability. Strain improvement and different culture conditions were studied for more effective production of digoxin. Methods and Results Among the isolated fungi, an isolate produced digoxin in a concentration of 2·07 mg l−1. The digoxin‐producing fungal isolate was identified as Epicoccum nigrum Link according to the morphological features and phylogenetic analyses. The potentiality of the fungal strain for production enhancement of digoxin was performed by gamma radiation mutagenesis. Gamma irradiation dose of 1000 Gy intensified the digoxin yield by five‐fold. Using this dose, a stable mutant strain with improved digoxin productivity was isolated and the stability for digoxin production was followed up across four successive generations. In the effort to increase digoxin magnitude, selection of the proper cultivation medium, addition of some elicitors to the most proper medium and several physical fermentation conditions were tested. Fermentation process carried out in malt extract autolysate medium (pH 6·5) supplemented by methyl jasmonate and inoculated with 2 ml of 6‐day‐old culture and incubated at 25°C for 10 days stimulated the highest production of digoxin to attain 50·14 mg l−1. Moreover, cytotoxicity of digoxin separated from the fungal culture was tested against five different cancer cell lines. Based on the MTT assay, digoxin inhibited the proliferation of the five different cancer cell lines and the recorded 50% inhibitory concentration ranged from 10·76 to 35·14 μg ml−1. Conclusions This is the first report on the production and enhancement of digoxin using fungal fermentation as a new and alternate source with high productivity. Significance and Impact of the Study These findings offer new and alternate sources with excellent biotechnological potential for digoxin production by fungal fermentation. Moreover, digoxin proved to be a promising anticancer agent whose anticancer potential should be assessed in prospective cancer therapy.

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“…It induces alterations in the genes of cells through the DNA repair mechanisms within the cells (Thacker ). In literature, several reports recommended both types of radiation in the production enhancement of many fungal metabolites (Paster et al ; Thaker et al ; Chopra ; Ismaiel et al , ; El‐Sayed et al . ,b,c,d; El‐Sayed et al ,b).…”

Section: Discussionmentioning

confidence: 99%

Agro‐industrial wastes for production of paclitaxel by irradiated Aspergillus fumigatus under solid‐state fermentation

2020

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Aims: Paclitaxel is the most profitable drug ever developed in cancer chemotherapy; however, the yield of paclitaxel from microbial platforms is still far from the commercial purpose. Thus, this study was conducted to explore the possibility of solid-state fermentation (SSF) for production of paclitaxel by fungal fermentation. Methods and Results: Different agro-industrial wastes were screened as solid substrates for production of paclitaxel by the endophytic Aspergillus fumigatus TXD105 under SSF. Sugarcane bagasse followed by wheat bran, and rice bran were the most suitable substrates for maximum production of paclitaxel. In the effort to increase the paclitaxel production, selection of the most proper moistening agent that supports the production of paclitaxel by the fungal strain was investigated. The effect of varying inoculum concentrations on the production of paclitaxel was also studied. Moreover, optimization of SSF conditions (moisture level, substrate concentrations and nutrients concentration) was adopted using response surface methodology. SSF carried out under the optimum conditions of 20 g sugarcane bagasse, twofold nutrients concentration of the MM1D broth, 80% moisture level and inoculum concentration of 10 7 spores per ml intensified the paclitaxel concentration to 145Á61 mg kg À1 which represents a 10-fold increase. The production of paclitaxel by the fungal strain was further improved via exposure to UV and gamma radiation at specific doses. The paclitaxel concentrations were intensified following UV and gamma radiation to 209Á91 and 351Á82 mg kg À1 . Conclusions: This is the first report on the production of paclitaxel using agro-industrial wastes as cheap source that may contribute in lowering the cost of producing paclitaxel. Significance and Impact of the Study: These findings offer new and alternate sources with excellent biotechnological potential for paclitaxel production by fungal fermentation.

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Immobilization technique for enhanced production of the immunosuppressant mycophenolic acid by ultraviolet and gamma-irradiated Penicillium roqueforti

Abstract: These findings indicate the future possibility to reduce the cost of producing fermentation-based drugs.

Cited by 24 publications

References 46 publications

Nanoencapsulation of Curcumin and Its Protective Effects against CCl₄-Induced Hepatotoxicity in Mice

Nanoencapsulation of Curcumin and Its Protective Effects against CCl₄-Induced Hepatotoxicity in Mice

A novel source of the cardiac glycoside digoxin from the endophytic fungus Epicoccum nigrum : isolation, characterization, production enhancement by gamma irradiation mutagenesis and anticancer activity evaluation

Agro‐industrial wastes for production of paclitaxel by irradiated Aspergillus fumigatus under solid‐state fermentation

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Immobilization technique for enhanced production of the immunosuppressant mycophenolic acid by ultraviolet and gamma-irradiated Penicillium roqueforti

Abstract: These findings indicate the future possibility to reduce the cost of producing fermentation-based drugs.

Cited by 24 publications

References 46 publications

Nanoencapsulation of Curcumin and Its Protective Effects against CCl4-Induced Hepatotoxicity in Mice

Nanoencapsulation of Curcumin and Its Protective Effects against CCl4-Induced Hepatotoxicity in Mice

A novel source of the cardiac glycoside digoxin from the endophytic fungus Epicoccum nigrum : isolation, characterization, production enhancement by gamma irradiation mutagenesis and anticancer activity evaluation

Agro‐industrial wastes for production of paclitaxel by irradiated Aspergillus fumigatus under solid‐state fermentation

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Nanoencapsulation of Curcumin and Its Protective Effects against CCl₄-Induced Hepatotoxicity in Mice

Nanoencapsulation of Curcumin and Its Protective Effects against CCl₄-Induced Hepatotoxicity in Mice