Immune effect of Vibrio harveyi formalin-killed cells vaccine combined with chitosan oligosaccharide and astragalus polysaccharides in ♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatus

Wei, Guangben; Cai, Shuanghu; Wu, Yuanzhi; Ma, Shaohong; Huang, Yucong

doi:10.1016/j.fsi.2020.01.015

Cited by 39 publications

(21 citation statements)

References 39 publications

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“…At 35 days post‐injection of COS, all fish were intraperitoneally challenged with 4.53 × 10 8 CFU/ml V. harveyi virulent strain ZJ0603 (isolated from the diseased grouper) (twofold LD50 of pearl gentian grouper) (Wei et al., 2020) at a dose of 200 μl. During the periods of 14 days post‐challenge of V. harveyi , the mortality of each group was recorded and the relative per cent rate (RPS) was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…The pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus ) is widely chosen to culture in China because of its important economic value and growing market demand. However, pearl gentian grouper culture was severely hampered by vibriosis, which caused dramatic economic loss (Ping‐Chung et al., 1996; Wei et al., 2020; X. H. Zhang & Austin, 2000). Vibrio spp.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies showed that feed diet with COS as immunostimulant could enhance immunity in aquatic animals, such as turbot ( Scophthalmus maximus L.) (Cui et al., 2013), tilapia (♀ Oreochromis niloticus × ♂ Oreochromis aureus ) (Qin et al., 2014), Penaeus monodon (Niu et al., 2013) and largemouth bass ( Micropterus salmoides ) (Lin et al., 2017). Some studies also showed aquatic animals injected vaccine combined with COS as adjuvant also improved the immunity, such as grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus ) (Wei et al., 2020) and zebrafish ( Danio rerio ) (Liu et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

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The effect of chitosan oligosaccharide as an immune enhancer against Vibrio harveyi in pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus )

et al. 2020

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of chitosan oligosaccharide as an immune enhancer against Vibrio harveyi in pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus )

et al. 2020

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“…Chitosan enhanced incorporation into the host cells and improved fish survival rate and immune response, increasing IgM content, lysozyme activity and mRNA levels of interleukin (IL)-1β, IL-2 and interferon (IFN)-γ2 [133,134]. A mixture of COS and inactivated Vibrio anguillarum vaccine significantly reduced zebrafish mortality against Vibro anguillarum [135], while COS combined with inactivated Vibrio harveyi also markedly increased survival rate, IgM and the expression of immune-related genes, such as IL-1β, IL-16, tumor necrosis factor-alpha (TNF-α) and major histocompatibility complex class I alpha (MHC-Iα), in the grouper Epinephelus fuscoguttatus×Epinephelus lanceolatus [136]. Similarly, rainbow trout (Oncorhynchus mykiss) immunized against bacterial infection (Lactococcus garvieae and Streptococcus iniae) through chitosan-alginate coated vaccination exhibited a higher survival rate, immune-related gene expression, and antibody titer than fish submitted to non-coated vaccination [137].…”

Section: Chitosan Loading Inactivated Pathogensmentioning

confidence: 99%

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology

Rashidpour

Pablos

et al. 2020

Polymers

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Chitosan is increasingly used for safe nucleic acid delivery in gene therapy studies, due to well-known properties such as bioadhesion, low toxicity, biodegradability and biocompatibility. Furthermore, chitosan derivatization can be easily performed to improve the solubility and stability of chitosan–nucleic acid polyplexes, and enhance efficient target cell drug delivery, cell uptake, intracellular endosomal escape, unpacking and nuclear import of expression plasmids. As in other fields, chitosan is a promising drug delivery vector with great potential for the fish farming industry. This review highlights state-of-the-art assays using chitosan-based methodologies for delivering nucleic acids into cells, and focuses attention on recent advances in chitosan-mediated gene delivery for fish biotechnology applications. The efficiency of chitosan for gene therapy studies in fish biotechnology is discussed in fields such as fish vaccination against bacterial and viral infection, control of gonadal development and gene overexpression and silencing for overcoming metabolic limitations, such as dependence on protein-rich diets and the low glucose tolerance of farmed fish. Finally, challenges and perspectives on the future developments of chitosan-based gene delivery in fish are also discussed.

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“…The liveattenuated vaccine offered safety for up to 83.7% of survival after a bacterial challenge with wild-type A. hydrophila. Another study with successful fish immunization is a study by Wei et al [26], which developed a V. harveyi formalin-killed cells vaccine against V. harveyi in hybrid grouper Epinephelus fuscoguttatus × E. lanceolatus. The vaccine provided more than 90% protection against the bacteria.…”

Section: Introductionmentioning

confidence: 99%

Vaccine Efficacy of a Newly Developed Feed-Based Whole-Cell Polyvalent Vaccine against Vibriosis, Streptococcosis and Motile Aeromonad Septicemia in Asian Seabass, Lates calcarifer

et al. 2021

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Multiple infections of several bacterial species are often observed under natural farm conditions. The infections would cause a much more significant loss compared to a single infectious agent. Vaccination is an essential strategy to prevent diseases in aquaculture, and oral vaccination has been proposed as a promising technique since it requires no handling of the fish and is easy to perform. This research attempts to develop and evaluate a potential feed-based polyvalent vaccine that can be used to treat multiple infections by Vibrios spp., Streptococcus agalactiae, and Aeromonas hydrophila, simultaneously. The oral polyvalent vaccine was prepared by mixing formalin-killed vaccine of V. harveyi, S. agalactiae, and A. hydrophila strains with commercial feed pellet, and palm oil as an adjuvant was added to improve their antigenicity. Thereafter, a vaccinated feed pellet was tested for feed quality analysis in terms of feed stability in water, proximate nutrient analysis, and palatability, safety, and growth performance using Asian seabass, Lates calcarifer as a fish host model. For immune response analysis, a total of 300 Asian seabass juveniles (15.8 ± 2.6 g) were divided into two groups in triplicate. Fish of group 1 were not vaccinated, while group 2 was vaccinated with the feed-based polyvalent vaccine. Vaccinations were carried out on days 0 and 14 with oral administration of the feed containing the bacterin at 5% body weight. Samples of serum for antibody and lysozyme study and the spleen and gut for gene expression analysis were collected at 7-day intervals for 6 weeks. Its efficacy in protecting fish was evaluated in aquarium challenge. Following vaccination by the polyvalent feed-based vaccine, IgM antibody levels showed a significant (p < 0.05) increase in serum against Vibrio harveyi, Aeromonas hydrophila, and Streptococcus agalactiae and reached the peak at week 3, 5, and 6, respectively. The high-stimulated antibody in the serum remained significantly higher than the control (p < 0.05) at the end of the 6 weeks vaccination trial. Not only that, but the serum lysozyme level was also increased significantly at week 4 (p < 0.05) as compared to the control treatment. The immune-related gene, dendritic cells, C3, Chemokine ligand 4 (CCL4), and major histocompatibility complex class I (MHC I) showed significantly higher expression (p < 0.05) after the fish were vaccinated with the oral vaccine. In the aquarium challenge, the vaccine provided a relative percentage survival of 75 ± 7.1%, 80 ± 0.0%, and 80 ± 0.0% after challenge with V. harveyi, A. hydrophila, and S. agalactiae, respectively. Combining our results demonstrate that the feed-based polyvalent vaccine could elicit significant innate and adaptive immunological responses, and this offers an opportunity for a comprehensive immunization against vibriosis, streptococcosis, and motile aeromonad septicemia in Asian seabass, Lates calcarifer. Nevertheless, this newly developed feed-based polyvalent vaccination can be a promising technique for effective and large-scale fish immunization in the aquaculture industry shortly.

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Immune effect of Vibrio harveyi formalin-killed cells vaccine combined with chitosan oligosaccharide and astragalus polysaccharides in ♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatus

Cited by 39 publications

References 39 publications

The effect of chitosan oligosaccharide as an immune enhancer against Vibrio harveyi in pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus )

The effect of chitosan oligosaccharide as an immune enhancer against Vibrio harveyi in pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus )

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology

Vaccine Efficacy of a Newly Developed Feed-Based Whole-Cell Polyvalent Vaccine against Vibriosis, Streptococcosis and Motile Aeromonad Septicemia in Asian Seabass, Lates calcarifer

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