Immune gene mining by pyrosequencing in the rockshell, Thais clavigera

Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein

“…A galectin (Aigalctin) of unique CRD organization from the bay scallop Argopecten irradians, can recognize a great number of environmental bacterial species and strains (Song et al, 2011). Another two galectins with quadruple CRDs were characterized from pearl oyster Pinctada fucata (Pfgalectin) and eastern oyster Crassostrea virginica, and their mRNA were remarkably upregulated by pathogens challenge (Liu et al, 2013;Rhee et al, 2012). A similar galectin (Mjgalectin) that is up-regulated by microbial challenge and can recognized microbial pathogens described in the kuruma shrimp Marsupenaeus japonicas (Shi et al, 2014).…”

Antioxidant activity of fractions isolated from hemolymph of garden snail Helix lucorum

“…The molecular ion [M+H] + at m/z 24 937.218 Da probably corresponds to the GPx determined in Haliotis discus with MW 24945 Da (A0A0H3V5M4_HALDI) and with MW 24 809 Da (A0A0H3V5S1_HALDI) (Bathige et al, 2015), as well as in Haliotis diversicolor supertexta (Cai and Zhou, 2010) with MW 24 989 Da (D6PW93_ HALDV). More of the enzymes belonging to the GPx family are extracellular proteins and in various gastropods (Biomphalaria glabrata (freshwater snail), Aplysia californica (California sea hare), Reishia clavigera (Sea snail Purpura clavigera), as well as abalones H. diversicolor supertexta and H. discus, with a molecular weight in the range between 20.0-27.5 kDa) are dissolved in the hemolymph (Moroz et al, 2006;Cai and Zhou, 2010;Rhee et al, 2012;Warren et al, 2013;Bathige et al, 2015).…”