Immune Modulation by IL-10 Gene Transfer via Viral Vector and Plasmid DNA: Implication for Gene Therapy

Interleukin-10 Gene Therapy Attenuates Pulmonary Tissue Injury Caused by Mesenteric Ischemia-Reperfusion in a Mouse Model

“…We selected plasmid DNA encoding human IL-10 as a therapeutic gene because of its detectability in the circulation of animals by using commercially available ELISA kits. Liposomal gene transfer method is preferred regarding the study of Chun et al (1999) that this type of gene expression was far more persistent than viral vector system. Also the systemic injection of DNA: liposome complexes into animals appears nontoxic.…”

Amelioration of established collagen induced arthritis by systemic IL-10 gene delivery

“…36,39 By comparison, intramuscular injection of naked plasmid encoding IL-10 or TGF␤ resulted in long-term suppression of cutaneous inflammatory lesions or experimental arthritis. 40,41 This suggests that a more effective approach to the treatment of chronic inflammatory conditions may be the use of a system that avoids immune reactions.…”

Immunomodulation by topical particle-mediated administration of cytokine plasmid DNA suppresses herpetic stromal keratitis without impairment of antiviral defense

“…The primers used were: β-gal1: 5′-ACT TTG ACC AAC AAG AA CGT G-3′, β-gal2: 5′-AAA TGT GAG CGA GTA ACA ACC-3′ [9,10].…”