Immune modulation mediated by extracellular vesicles of intestinal organoids is disrupted by opioids

Zhang, Yue; Yan, Yan; Meng, Jingjing; Girotra, Mohit; Ramakrishnan, Sundaram; Roy, Sabita

doi:10.1038/s41385-021-00392-9

Cited by 19 publications

(17 citation statements)

References 31 publications

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“…Specifically, the Let-7 family of miR-NAs were downregulated in EVs produced by morphine-treated organoids [83]. While miR-146a levels have been shown to be altered by morphine treatment, in this study they were unchanged in the EVs, suggesting other mechanisms may be at play in modifying miR-146a expression [83].…”

Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 53%

“…While EVs from intestinal organoids without morphine treatment were shown to alleviate LPS-mediated inflammation in vitro, EVs from morphine-exposed organoids did not [83]. Specifically, the Let-7 family of miR-NAs were downregulated in EVs produced by morphine-treated organoids [83]. While miR-146a levels have been shown to be altered by morphine treatment, in this study they were unchanged in the EVs, suggesting other mechanisms may be at play in modifying miR-146a expression [83].…”

Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 92%

“…Interestingly, many opioid-induced extravesicular miRNAs are associated with TLR signaling, specifically the Let-7 miRNA family and miR-146. While EVs from intestinal organoids without morphine treatment were shown to alleviate LPS-mediated inflammation in vitro, EVs from morphine-exposed organoids did not [83]. Specifically, the Let-7 family of miR-NAs were downregulated in EVs produced by morphine-treated organoids [83].…”

Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 95%

“…Recently, a study using intestinal organoids has shed insight into the morphine modulation of miRNAs through extracellular vesicles (EVs) in response to LPS [83]. EVs produced by intestinal epithelial cells deliver miRNAs and other signaling molecules to intestinal immune cells to coordinate homeostasis or the immune response [84,85].…”

Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 99%

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Opioids and Sepsis: Elucidating the Role of the Microbiome and microRNA-146

Abu

Vitari

Yan

et al. 2022

IJMS

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Sepsis has recently been defined as life-threatening organ dysfunction caused by the dysregulated host response to an ongoing or suspected infection. To date, sepsis continues to be a leading cause of morbidity and mortality amongst hospitalized patients. Many risk factors contribute to development of sepsis, including pain-relieving drugs like opioids, which are frequently prescribed post-operatively. In light of the opioid crisis, understanding the interactions between opioid use and the development of sepsis has become extremely relevant, as opioid use is associated with increased risk of infection. Given that the intestinal tract is a major site of origin of sepsis-causing microbes, there has been an increasing focus on how alterations in the gut microbiome may predispose towards sepsis and mediate immune dysregulation. MicroRNAs, in particular, have emerged as key modulators of the inflammatory response during sepsis by tempering the immune response, thereby mediating the interaction between host and microbiome. In this review, we elucidate contributing roles of microRNA 146 in modulating sepsis pathogenesis and end with a discussion of therapeutic targeting of the gut microbiome in controlling immune dysregulation in sepsis.

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Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 53%

Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 92%

Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 95%

Section: Opioid Modulation Of Immune Cells May Increase Sepsis Riskmentioning

confidence: 99%

See 2 more Smart Citations

Opioids and Sepsis: Elucidating the Role of the Microbiome and microRNA-146

Abu

Vitari

Yan

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

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“…Irritants, drugs, or components in foods can alter the production and properties of gut-released EVs [63][64][65]. These EVs may cross the BBB and influence the physiology and behavior of the brain.…”

Section: Gut-released Evsmentioning

confidence: 99%

Organ-on-a-Chip for Studying Gut-Brain Interaction Mediated by Extracellular Vesicles in the Gut Microenvironment

Kim

Noort

Sung

et al. 2021

IJMS

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Extracellular vesicles (EVs) are a group of membrane vesicles that play important roles in cell-to-cell and interspecies/interkingdom communications by modulating the pathophysiological conditions of recipient cells. Recent evidence has implied their potential roles in the gut–brain axis (GBA), which is a complex bidirectional communication system between the gut environment and brain pathophysiology. Despite the evidence, the roles of EVs in the gut microenvironment in the GBA are less highlighted. Moreover, there are critical challenges in the current GBA models and analyzing techniques for EVs, which may hinder the research. Currently, advances in organ-on-a-chip (OOC) technologies have provided a promising solution. Here, we review the potential effects of EVs occurring in the gut environment on brain physiology and behavior and discuss how to apply OOCs to research the GBA mediated by EVs in the gut microenvironment.

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Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Moura

Monteiro

Ferreira

et al. 2022

Adv Funct Materials

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The extracellular matrix plays a critical role in bioinstructing cellular self‐assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein is showcased the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprints, characterization methodologies, and extracellular matrix processing toolboxes are comprehensively discussed in light of current advances. Such enabling approaches provide the grounds for engineering next‐generation tissue‐specific hydrogels with close‐to‐native biomolecular signatures and user‐tailored biophysical properties that may potentiate organoids physiomimetic potential. In a forward looking perspective, the combination of tissue‐specific decellularized hydrogels with increasingly complex multicellular assemblies and bottom‐up cell engineering technologies may unravel unprecedented tissue‐like physiological responses and further advance the exploitation of organoids and assembloids as human disease surrogates or as patient‐tailored living therapeutics.

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Immune modulation mediated by extracellular vesicles of intestinal organoids is disrupted by opioids

Cited by 19 publications

References 31 publications

Opioids and Sepsis: Elucidating the Role of the Microbiome and microRNA-146

Opioids and Sepsis: Elucidating the Role of the Microbiome and microRNA-146

Organ-on-a-Chip for Studying Gut-Brain Interaction Mediated by Extracellular Vesicles in the Gut Microenvironment

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

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