Immune response against Streptococcus gallolyticus in patients with adenomatous polyps in colon

Abstract

Our aim was to examine the humoral immune response against Streptococcus gallolyticus subspecies gallolyticus antigens in individuals subjected to a routine colonoscopy in which colon adenomatous polyps were present or not. Serum samples from 133 individuals with adenomatous polyps and serum samples from 53 individuals with a normal colonoscopy were included. Western blot was performed in all subjects using a whole cell antigen from S. gallolyticus ATCC 9809, and rabbit antisera against the whole cell bacteria was prepared as a control. By analyzing the immune profile of the rabbit‐immunized sera by Western‐blot, at least 22 proteins were identified as immunogenic in S. gallolyticus. When we evaluated sera from human subjects, two proteins of approximately 30 and 22 kDa were most prominent. Based on this 2‐protein band pattern, Western‐blot profiles from human subjects were compared. The detection of a protein band of 22 kDa was associated with the presence of adenomatous polyps in colon [odds ratios (OR) 7.98, 95% confidence intervals (CI): 3.54–17.93], p < 0.001. When the presence of the 30 kDa protein alone or both the 22 and 30 kDa proteins were analyzed, the OR increased to 22.37 (95% CI: 3.77–131.64), p < 0.001. The specificity was 84.9 for the presence of the 22 kDa protein, and 98.1 for the presence of the 30 kDa protein alone or both 22 and 30 kDa bands. Serum from individuals with adenomatous polyps recognized two proteins from S. gallolyticus. This result confirmed the possible association of S. gallolyticus with adenomatous polyps in the colon.

Prospective evaluation of antibody response to Streptococcus gallolyticus and risk of colorectal cancer

“…14 In the German case-control study, the SGG multiplex serology panel was extended to the 11 SGG proteins used in this study. 11,12,16,19 Here, we offer the first prospective observational evidence to support early involvement of SGG in colon carcinogenesis by showing that antibody responses to SGG were more frequently present in subjects who later developed CRC even more than two years after blood draw than those who remained disease-free during the same time-frame. Seropositivity for at least two proteins from a 6-marker panel subset (Gallo0272, Gallo0748, Gallo1675, Gallo2018, Gallo2178 and Gallo2179) was more specifically associated with CRC (19% SGG positives) compared to controls (11% of SGGpositives).…”

“…In the 1970s, it was found that infective endocarditis What's new? [11][12][13][14] We previously applied multiplex serology, a fluorescent bead-based high-throughput technology allowing serological typing of several antigens in one reaction, 15 to analyze antibody responses to 11 SGG proteins in a German CRC casecontrol study. Here the authors performed a serological study in a prospective setting with samples from the European Prospective Investigation into Nutrition and Cancer (EPIC).…”

Genomics, evolution, and molecular epidemiology of the Streptococcus bovis / Streptococcus equinus complex (SBSEC)

“…Furthermore, such antigen profiles of Sgg were found to be clearly associated with CRC already present at early polyp status suggesting early involvement of Sgg into the tumour environment (Tjalsma et al, 2006). Major protein antigens of Sgg were identified as HlpA and Rp L7/L12 (Boleij et al, 2010;Tjalsma et al, 2007;Tjalsma et al, 2006), in addition to two unidentified antigens of 22 and 30 kDa size (Garza-González et al, 2012), suggesting further potential for diagnostic tools to detect SBSEC.…”

Novel Real-Time PCR Assays Using TaqMan Minor Groove Binder Probes for Identification of Fecal Carriage of Streptococcus bovis/Streptococcus equinus Complex from Rectal Swab Specimens

“…is more frequently related to both endocarditis and colonic lesions than S. infantarius sp. (4,5). Nevertheless, S. infantarius sp.…”