Immune Response Suppression by an Inhibitor in Normal and Immune Mouse Serum

Veit, Bruce C.; Michael, J. G.

doi:10.1038/newbio235238a0

Cited by 38 publications

(11 citation statements)

References 8 publications

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“…These factors are not limited to cancer, however. Similar factors have been detected in blood and urine in a variety of conditions, including experimental amyloidosis [3], pregnancy [4, 51, liver disease [6], and have been found in normal and immune mouse sera [7] and normal human serum [&12]. It is Eur.…”

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confidence: 57%

Purification and characterization of an immunosuppressive factor from ovarian cancer ascites fluid

Moolten

1981

Eur J Immunol

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A nonspecific immunosuppressive factor present in malignant (ovarian carcinoma) ascites fluid has been purified by acid extraction from a high molecular weight (greater than 20000) complex followed by preparative isoelectric focusing on Bio-lyte media. It is an acidic protein (pI = 3.6) of mol. wt. 50000 to 52000 as estimated by gel filtration and composed of subunits of 25000 to 26000 estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, under reducing conditions. It inhibits the phytohemagglutinin-dependent mitogenic response of normal peripheral blood lymphocytes by 50% at 2 microgram/ml concentrations in vitro and suppresses 80% of the plaque-forming cell response to sheep erythrocytes at 100 microgram per mouse in vivo. Its chemical identity with any of the known plasma proteins has not been established. Its failure to stain with periodic acid Schiff's reagent indicates its minimal content of carbohydrates. It is susceptible to tryptic and pronase digestion but insensitive to deoxyribonuclease and ribonuclease digestion. A smaller suppressive factor identified in the same fluid appears to be a lymphotoxin; it differs from the acid-extracted nonspecific suppressive factor in its lack of susceptibility to trypsin.

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confidence: 57%

Purification and characterization of an immunosuppressive factor from ovarian cancer ascites fluid

Moolten

1981

Eur J Immunol

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“…Veit and Michael [21] reported that immunosuppressive activity of mouse serum is lost following treatment at 65 ° C for 30 rain. Gundersen et al [6] also reported a heatlabile (56 ° C, 30 min) antiproliferative activity in mouse serum.…”

Section: Discussionmentioning

confidence: 99%

Effects of injection routes of growing tumors PSK or OK-432 on antiproliferative activity of mouse serum

Ryoyama

1989

Cancer Immunol Immunother

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The present study examined the effects of various treatments on the antiproliferative activity of mouse serum. Its activity was estimated against the growth of EL4 tumor cells and L929 cells and against splenic blastogenesis in culture. The activity varied among mouse strains tested and among individuals in any strain. However, normal outbred NIH Swiss mice showed the highest activity among the strains and the least variation among individuals. The activity of serum from NIH Swiss mice constantly decreased 7 or 14 days after an injection of 10(6) Ehrlich or sarcoma 180 tumor cells subcutaneously in the right-hind footpad, intradermally in the right side of the chest or into the palm. Other routes, such as intraperitoneal, intravenous in the tail vein, subcutaneous in the right side of the chest and intramuscular in the left thigh, however, hardly affected the activity. The activity also decreased 7 days after an injection into the footpad of a biological response modifier such as PSK or OK-432. The antiproliferative activity of mouse serum seem to depend on macrophages but not natural killer-cell activity, because treatment with silica but not anti-(asialo-GM1) antibody totally reduced the activity. The active fraction was heat-stable (100 degrees C, 30 min) and its molecular mass was 127-140 kDa.

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“…cell-mediated im munity and T-dependcnt antibody respon ses. While prolongation of alio-and xeno graft survival was reported [11,17,[21][22][23] as well as inhibition of anti-SRBC response [5,6,10,18,28], no influence has been found using lipopolysaccharide, a T-independent antigen [18,20,28]. Our experi ments clearly confirm and extend observa tions by others that fraction C of «-globulins of different origin (human and bovine sera, mouse ascitic fluid, human ovarian cyst flu id) substantially reduces also other cell-me diated phenomena -GVH reaction and contact sensitivity, while neither fraction A or B are effective.…”

Section: Discussionmentioning

confidence: 99%

“…It has been argued that «-globulins present in the serum at the threshold concentration serve as a control system regulating the immune response [28], and constitute a feedback mechanism involved in the regulation of T lymphocyte function [3,4,18,28], akin to antibody-me diated suppression of antibody response. In several pathological conditions raised, «-globulin level is paralleled by a deficient T lymphocyte function [12,24].…”

Section: Discussionmentioning

confidence: 99%

“…the antigen-or PHA-induced blastogenesis of lymphocytes [7,8,20,24,25], recruitment of plaque forming cells [6,28], and abolishes the anti gen-induced elaboration of MIF [9|. In vivo «-globulins suppress, e.g., both primary and secondary immune responses to SRBC [6,10], as well as the rejection of skin [11,16,17,22,23] and tumour grafts [12].…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effect of α-Globulins of Different Origin on the Cell-Mediated Immune Responses in Mice

Ptak

Gumińska

Stachurska

et al. 1977

Int Arch Allergy Immunol

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α-Globulins prepared from different biological sources (human and bovine sera, mouse ascitic fluid, human ovarian cyst fluid) were separated by ion exchange chromatography on DEAE-cellulose into fractions A, B and C. Whereas fractions A and B had no immunosuppressive activity, fraction C injected into mice shortly before antigen administration, but not after, significantly inhibited the contact sensitivity to oxazolone. Single high doses were preferential over divided doses given on several occasions. In transfer experiments when cells of animals sensitized to oxazolone were injected into recipients treated previously with fraction C, the response was also inhibited. Fraction C, when administered into F1 hybrid mice before injection of parental splenocytes, prevented the development of GVH reaction. The authors point out that the critical concentration of α-globulins at the time of antigen administration is necessary to make T lymphocytes hyporesponsive, and when once triggered by antigen they become refractory to α-globulins action.

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Immune Response Suppression by an Inhibitor in Normal and Immune Mouse Serum

Cited by 38 publications

References 8 publications

Purification and characterization of an immunosuppressive factor from ovarian cancer ascites fluid

Purification and characterization of an immunosuppressive factor from ovarian cancer ascites fluid

Effects of injection routes of growing tumors PSK or OK-432 on antiproliferative activity of mouse serum

Inhibitory Effect of α-Globulins of Different Origin on the Cell-Mediated Immune Responses in Mice

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