Immune response to Vi polysaccharide, heat-killed whole cells, and outer membrane protein of Salmonella typhi

Hosny, Alaa El-Din Shawky; Diab, Mohamed Reda; Khattab, Rania Abdelmonem; Awad, Heba Osama

doi:10.3855/jidc.6504

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Specifically, SseB and different Omp family members have been described as potential vaccine candidates in mice (200)(201)(202). SseB is also immunogenic in humans; indeed, SseB-specific CD4 ϩ T cells producing IFN-␥ are detected in the circulation of asymptomatic individuals or people previously exposed to S. Typhi (203).…”

Section: Salmonella Antigens and Their Use In Vaccine Formulationsmentioning

confidence: 99%

Vaccination against Salmonella Infection: the Mucosal Way

Gayet¹,

Bioley²,

Rochereau³

et al. 2017

Microbiol Mol Biol Rev

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subspecies includes several serovars infecting both humans and other animals and leading to typhoid fever or gastroenteritis. The high prevalence of associated morbidity and mortality, together with an increased emergence of multidrug-resistant strains, is a current global health issue that has prompted the development of vaccination strategies that confer protection against most serovars. Currently available systemic vaccine approaches have major limitations, including a reduced effectiveness in young children and a lack of cross-protection among different strains. Having studied host-pathogen interactions, microbiologists and immunologists argue in favor of topical gastrointestinal administration for improvement in vaccine efficacy. Here, recent advances in this field are summarized, including mechanisms of bacterial uptake at the intestinal epithelium, the assessment of protective host immunity, and improved animal models that closely mimic infection in humans. The pros and cons of existing vaccines are presented, along with recent progress made with novel formulations. Finally, new candidate antigens and their relevance in the refined design of anti- vaccines are discussed, along with antigen vectorization strategies such as nanoparticles or secretory immunoglobulins, with a focus on potentiating mucosal vaccine efficacy.

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Section: Salmonella Antigens and Their Use In Vaccine Formulationsmentioning

confidence: 99%

Vaccination against Salmonella Infection: the Mucosal Way

Gayet¹,

Bioley²,

Rochereau³

et al. 2017

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

show abstract

“…Furthermore, heat-killed aro-deficient Salmonella expressing fragment C (under control of the tac promoter)failed to induce immune responses (12). In contrast, many previous studies reported that heat-killed Salmonella is a potential vaccine against salmonellosis (2,(20)(21)(22). The source of these discrepancies is not known.…”

Section: Discussionmentioning

confidence: 87%

The Vaccine Potential of Heat-killed Attenuated Strain of <i>Salmonella</i>

Hashizume-Takizawa

2016

Int J Oral-Med Sci

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Live attenuated Salmonella strains are thought to be potential vaccines or vaccine vectors for delivery of recombinantly expressed vaccine antigens(Ags). A non-replicative recombinant mutant strain of Salmonella (rSalmonella) that expresses fragment C of tetanus toxin(Tox C)has been shown to be strongly immunogenic, causing induction of significant systemic and mucosal antibody(Ab)responses against the Tox C Ag. However, since previous studies reported that administration of live attenuated strains of Salmonella resulted in side effects, it is preferable to use inactivated bacterial vaccines. Thus, in this study, we assessed the immunogenicity of a heat-killed attenuated strain of rSalmonella. The results showed that oral immunization with heat-killed rSalmonella-Tox C induced tetanus toxoid(TT)-specific systemic IgM Ab responses at levels indistinguishable from those of mice administered live bacteria. However, rSalmonella-Tox C had to be administered at a 10-fold higher oral dose than live bacteria to produce similar levels of TTspecific IgM-forming cells. Even at this higher dose, heat-killed rSalmonella-Tox C induced TT-specific systemic IgG Ab responses that were significantly lower than those obtained in mice dosed with live bacteria. Furthermore, no anti-TT IgA response was detected in the intestinal secretions of mice that were orally dosed with heat-killed rSalmonella-Tox C. Markedly decreased numbers of TT-specific IgG-and IgA-producing cells were detected in the lymph nodes of mice immunized with rSalmonella-Tox C. These results suggest that heat-killed rSalmonella-Tox C may not be appropriate for use as an oral vaccine or vaccine vector.

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Production of electrolyzed water for home-use based on electrodeposited macroporous platinum

Kim

2017

J Mech Sci Technol

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Immune response to Vi polysaccharide, heat-killed whole cells, and outer membrane protein of Salmonella typhi

Cited by 6 publications

References 28 publications

Vaccination against Salmonella Infection: the Mucosal Way

Vaccination against Salmonella Infection: the Mucosal Way

The Vaccine Potential of Heat-killed Attenuated Strain of <i>Salmonella</i>

Production of electrolyzed water for home-use based on electrodeposited macroporous platinum

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