Immune responses and expression profiles of some immune-related genes in Indian major carp, Labeo rohita to Edwardsiella tarda infection

Mohanty, B.R.; Sahoo, P.K.

doi:10.1016/j.fsi.2009.12.025

Cited by 150 publications

(69 citation statements)

References 60 publications

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“…In this way, pacu challenged by A. hydrophila have shown improvement in ROSs production and increased protection against pathogens, assessed by the respiratory burst assay. The activation of immune mechanisms carries out an important function in preventing disease in fish and several studies have evaluated these fish responses after pathogen infection (Rodríguez et al, 2008;Raida and Buchmann, 2009;Mohanty and Sahoo, 2010;Reyes-Becerril et al, 2011b). Reyes-Becerril et al (2011a) and the second with 1 × 10 8 cell mL -1 , and found mainly respiratory burst activity increasing after the second infection, indicating recovery of the immune system after pathogen exposition.…”

Section: Resultsmentioning

confidence: 99%

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

et al. 2013

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The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions. Keywords: immune methodology, fish immunology, cellular immunity. Atividade respiratória de leucócitos como indicador de imunidade inata de pacu (Piaractus mesopotamicus)Resumo O presente estudo avaliou o ensaio para quantificar a atividade respiratória dos leucócitos do sangue de pacu como um indicador do sistema imune inato, usando a redução do nitroazul tetrazólio (NBT) a formazan como medida da produção de espécies reativas de oxigênio (EROs). Para avaliar a precisão do ensaio, peixes foram desafiados por Aeromonas hydrophila e amostrados uma semana após o desafio. A infecção com A. hydrophila aumentou a atividade respiratória dos leucócitos. O protocolo se mostrou confiável e de fácil aplicação, apropriado para determinar a atividade respiratória de leucócitos do sangue do pacu, peixe neotropical, nas condições experimentais apresentadas.Palavras-chave: metodologia, imunidade de peixe, imunidade mediada por células.

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Section: Resultsmentioning

confidence: 99%

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

et al. 2013

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“…Se realizó siguiendo la técnica espectrofotométrica descrita por Mohanty & Sahoo (17). Brevemente, se dispusieron de 50 µl de sangre en un vial con 50 µl de Nitroblue tetrazolium (NBT Tablet) 0.2% y se incubó durante 30 minutos a 25ºC.…”

Section: Colecta De Muestrasunclassified

Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

2016

Rev MVZ Córdoba

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El suplemento de sustancias húmicas afecta la inmunidad innata en gallinas ponedoras en fase posmuda ABSTRACTObjective. Asses the effect of supplementation with Humic substances (HS) over some innate immunity parameters (serum bactericidal activity, phagocytosis, bacterial agglutination, respiratory burst and lisozyme activity) in phase after fasting of layer hens. Materials and methods. 120 posfasting phase Hy Line Brown layer hens were taken which were distributed into four groups: The first and the second were supplemented with 0.1 and 0.2% of HS, respectively. The third group was supplemented with 0.25 mg/kg on levamisole hydrochloride and fourth group have no supplementation; during sixty days period. Blood samples were collected on 8th, 30th and 60th of experiment day. Results. The phagocytic index and respiratory burst increased significantly at day 30th in HS supplemented groups. Alike, serum bactericidal activity and lisozyme activity improved on 8 th day, nevertheless, changes were no evident latter. The bacterial agglutination was high in supplemented groups evaluated at everyone times. Conclusions. Results showed that HS behave as immunostimulant in the early phase after fasting layer hens.Key words: Agglutination, bactericide activity, Lisozyme, phagocytosis (Source: DeCS). RESUMENObjetivo. Evaluar el efecto de las sustancias húmicas (SH) sobre algunos parámetros de la inmunidad innata (actividad bactericida del suero, fagocitosis, aglutinación bacteriana, explosión respiratoria y actividad de la lisozima) en la fase posmuda de gallinas ponedoras. Materiales y métodos. Se utilizaron 120 gallinas ponedoras Hy Line Brown en la fase de posmuda, las cuales fueron divididas en cuatro grupos: Los dos primeros fueron suplementados con 0.1 y 0.2% de SH respectivamente, el tercer grupo fue suplementado con 0.25 mg/kg de Clorhidrato de levamisol y el cuarto grupo control sin suplemento; durante un período de 60 días. Las muestras sanguíneas se tomaron los días 8, 30 y 60 del experimento. Resultados. El Índice fagocítico y la explosión respiratoria se incrementaron significativamente a partir del día 30 de suplementación con SH. De la misma manera, la actividad bactericida del suero y la actividad de la lisozima aumentaron al día 8; no obstante no se evidenciaron cambios posteriores. La aglutinación bacteriana fue significativamente mayor en los grupos suplementados en todos los tiempos evaluados. Conclusiones. Los resultados demuestran que las SH se comportan como agentes inmunoestimulantes en la fase temprana de la posmuda en gallinas ponedoras Hy Line Brown. Palabras clave: Actividad bactericida, aglutinación, fagocitosis, lisozima (Fuente: DeCS).Rev.MVZ Córdoba 21(1):5198-5210, 2016. ISSN: 0122-0268 ORIGINAL 5199 INTRODUCCIÓNLos mecanismos de la respuesta inmune, innata o adaptativa durante una infección demandan un gasto energético que puede interferir con la capacidad productiva de animales susceptibles. Tradicionalmente, el abordaje de los problemas infecciosos incluye, además, de la disminución de los...

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“…Aquaculture is one of the fastest growing food-producing fields in the world, with an annual average growth rate of 6.9% per year since 1970 and this sector contributed about 36% of the total global fisheries production in the year 2006 (FAO, 2009;Mohanty & Sahoo, 2010). Infectious diseases are a major problem in aquaculture, causing heavy loss to fish farmers.…”

Section: Introductionmentioning

confidence: 99%

Specific and nonspecific immunostimulation study ofEuphorbia hirtaonPseudomonas fluorescens-infectedCyprinus carpio

Pratheepa

Sukumaran

2011

Pharmaceutical Biology

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Immune responses and expression profiles of some immune-related genes in Indian major carp, Labeo rohita to Edwardsiella tarda infection

Cited by 150 publications

References 60 publications

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

Specific and nonspecific immunostimulation study ofEuphorbia hirtaonPseudomonas fluorescens-infectedCyprinus carpio

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