Immune responses and protective efficacy of recombinant outer membrane protein R (rOmpR)-based vaccine of Aeromonas hydrophila with a modified adjuvant formulation in rohu (Labeo rohita)

Dash, Pujarini; Sahoo, P.K.; Gupta, Pankaj; Garg, Lalit C.; Dixit, Aparna

doi:10.1016/j.fsi.2014.06.007

Cited by 41 publications

(35 citation statements)

References 44 publications

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“…Higher IgM levels in OmpR-modified adjuvant/non-challenged fish point towards a longlived antibody response, along with elevated innate immune effectors, as noticed in this study. The rOmpR component of A. hydrophila might be able to mimic the pathogen itself, thus resulting in higher levels of protective antibody, as reflected in the postchallenge survival rate of rOmpR vaccinated groups (DASH et al, 2014). An elevated IgM titer was marked in A. hydrophila resistant common carp families, when challenged with the specific pathogen (ARDO et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Large scale production of rOmpR was carried out using pRSET.AhOmpR plasmid as per the protocol described previously (DASH et al, 2014). The mineral oil-based herbal modified adjuvant was prepared as described by DASH et al (2014). The bacterial isolate of A. hydrophila (Ah#15) P. Dash et al: Post-challenge immune response in rOmpR vaccinated rohu used in this study was isolated from a dermal ulcer of L. rohita during an outbreak, and was preserved in glycerol at -80 °C .…”

Section: Methodsmentioning

confidence: 99%

“…The modified adjuvant consisted of a combination of the widely used mineral oil and a herbal product, and was found to reduce the side effects of the mineral oil. The modified adjuvant, when used with the rOmpR, resulted in an enhanced immune response against the rOmpR of A. hydrophila, and reduced mortality significantly upon A. hydrophila challenge (DASH et al, 2014). In the present study, we attempted to explore the resistance mechanism in L. rohita, immunized with the modified adjuvant-based rOmpR vaccine and subsequently challenged with virulent A. hydrophila.…”

Section: Introductionmentioning

confidence: 99%

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Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

et al. 2017

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Although vaccination and post-vaccination studies have gained considerable importance in the field of aquaculture diseases, little is understood about the mechanism of immune-protection in vaccinated fish following infection. In the present study, an attempt was made to discover the mechanism of protection rendered by a modified adjuvant-based recombinant outer membrane protein R (rOmpR) vaccine, in rohu, Labeo rohita (an important Indian major carp species) following a challenge with Aeromonas hydrophila. For this, expression analysis of an array of immune molecules (IgM, complement factor 3, interleukin 1β, interleukin 6, tumor necrosis factor α, interleukin 15, MHC I and MHC II) was carried out in the anterior kidney tissues of vaccinated fish, at 0, 12, 24, 48, 72 and 120 h post-challenge. Higher transcript levels of IgM, MHC I and MHC II were evident in the mineral oil-based OmpR vaccinated and modified adjuvant-based OmpR-vaccinated groups, at different time points post-challenge, including the non-challenged fish. Early onset of pro-inflammatory cytokines and expression of most of the immune genes was more pronounced in vaccinated fish at different time points post-challenge, thus indicating a better immune response in these fish. This study indicated the activation of both humoral and cell mediated immunity in vaccinated rohu following a challenge with A. hydrophila.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

et al. 2017

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“…Primer sequences and their optimum annealing temperatures are given in Table 1. All the amplifications were carried out using 1 µl of reversetranscribed cDNA as detailed elsewhere [18]. The generated PCR products were purified by PCR product purification kit (Bangalore Genei, Bangalore) and were cloned into T cloning vector using an instant cloning kit (Bangalore Genei, Bangalore) according to manufacturer's instructions.…”

Section: Pcr Cloning and Cytokine Expression Analysismentioning

confidence: 99%

Expression profile of pro-inflammatory cytokines in head-kidney leucocytes of rohu following stimulation with poly I:C

Sahoo¹

2017

IJMBR

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IL (interleukin)-6, TNF (tumor necrosis factor)-α, IL-1β and IL-8 are pro-inflammatory cytokines which augment host inflammatory reactions in response to multiple stresses or infections. In this study, we analyzed expression profile of these cytokines in head kidney leucocytes (HKL) isolated from healthy rohu (Labeo rohita) juveniles in response to poly I:C (50 µg/ml), a synthetic dsRNA virus analog stimulation. RNA was isolated from stimulated HKLs at 0, 1, 3, 6, 12, 24, and 72 and 96 h post-stimulation (hps) and the expression profile of IL-6, TNF-α, IL-1β and IL-8 was studied using qPCR. IL-6 expression was found to be significantly up-regulated at 72 hps whereas higher level of TNF-α was evident at 96 hps. With regard to IL-8 and IL-1β, a similar kind of expression pattern was observed where levels of both cytokines were higher at 24 hps. As activation of pro-inflammatory cytokine cascade against infection is considered as a marker of early immunity, induction of cytokine levels at or after 24 hps in rohu HKLs following poly I:C stimulation suggested the potential inflammatory role played by these cytokines during viral infection in fish.

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Aeromonadaceae Representatives (Motile Aeromonads)

Austin¹,

Austin²

2016

Bacterial Fish Pathogens

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Immune responses and protective efficacy of recombinant outer membrane protein R (rOmpR)-based vaccine of Aeromonas hydrophila with a modified adjuvant formulation in rohu (Labeo rohita)

Cited by 41 publications

References 44 publications

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Expression profile of pro-inflammatory cytokines in head-kidney leucocytes of rohu following stimulation with poly I:C

Aeromonadaceae Representatives (Motile Aeromonads)

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Immune responses and protective efficacy of recombinant outer membrane protein R (rOmpR)-based vaccine of Aeromonas hydrophila with a modified adjuvant formulation in rohu (Labeo rohita)

Cited by 41 publications

References 44 publications

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Expression profile of pro-inflammatory cytokines in head-kidney leucocytes of rohu following stimulation with poly I:C

Aeromonadaceae Representatives (Motile Aeromonads)

Contact Info

Product

Resources

About

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3

Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-basedohu, Aeromonas hydrophila outer membrane protein R formulation:l3