Immune Responses to Novel Pneumococcal Proteins Pneumolysin, PspA, PsaA, and CbpA in Adenoidal B Cells from Children

Zhang, Qibo; Choo, Sharon; Finn, Adam

doi:10.1128/iai.70.10.5363-5369.2002

Cited by 62 publications

(50 citation statements)

References 51 publications

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“…Since IgGproducing plasma cells are scarce in salivary glands, and it has been shown that that immunocytes, especially IgG-secreting cells, are present in significant numbers in surface secretion on adenoids) [35,36], this may represent an additional source of immunoglobulin in local mucosal secretions including saliva. We have previously reported that adenoids commonly contain significant numbers of antibody-secreting cells to the pneumococcal protein antigens studied here [28]. Another possibility is that contact between the sponge swab and the oral mucosa increased passive transudation from serum to a variable extent, thus explaining the unexpectedly high concentrations of IgG to some antigens in some individuals' saliva.…”

Section: Discussionmentioning

confidence: 94%

“…We have previously shown that antibody-secreting cells to pneumococcal protein antigens are present among adenoidal mononuclear cells (MNC) isolated from children undergoing adenoidectomy, suggesting that these pneumococcal proteins prime specific B cells for production of specific IgA, IgG and IgM antibodies in these children [28]. Here we report antibody responses to pneumococcal protein antigens in serum, saliva and adenoidal MNC after in vitro antigen stimulation in relation to carriage status in children.…”

Section: Introductionmentioning

confidence: 97%

“…Western blots of the CCS using specific antisera to CbpA, Ply, PsaA and PspA confirming the presence of these proteins, and further blots confirming the absence of CbpA or Ply in the CCS derived from the respective mutant strains were performed (Fig. 5) following methods described previously [28]. These CCS were subsequently used at a predetermined protein concentration of 1 lg/mL in cell stimulation experiments.…”

Section: Pneumococcal Culture Supernatants and Recombinant Proteinsmentioning

confidence: 99%

“…Adenoids were transported in minimum essential medium (Sigma, Dorset, UK) supplemented with glutamine, penicillin (100 U/mL), streptomycin (100 lg/mL) and amphotericin B (1 lg/mL) to the laboratory and processed within 1 h after operation following the methods described previously [28]. Briefly, adenoid tissue was minced using a sterile scalpel and teased with the help of a sterile steel mesh to release cells into the medium.…”

Section: Isolation Of Mnc From Adenoidsmentioning

confidence: 99%

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Serum and mucosal antibody responses to pneumococcal protein antigens in children:relationships with carriage status

et al. 2005

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Streptococcus pneumoniae causes significant morbidity and mortality especially in children. Some pneumococcal protein antigens can protect mice against infection. Little information is available concerning the nature of naturally acquired protective immunity to pneumococci in humans induced by these antigens. This study investigates the relationships between systemic and local antibody production and carriage in children. Children undergoing adenoidectomy (n=112, ages 2-12 years) were studied. Nasopharyngeal swabs were collected for pneumococcal culture. Serum and saliva were assayed for antibodies to several pneumococcal proteins: choline binding protein A (CbpA), pneumolysin (Ply), pneumococcal surface adhesin A (PsaA) and pneumococcal surface protein A (PspA). Adenoidal mononuclear cells (MNC) were cultured with pneumococcal culture supernatants or recombinant proteins. Cell culture supernatants were analyzed for antigen-specific antibodies. Carriage rates fell with age and serum levels of anti-CbpA, Ply and PspA rose. Anti-CbpA and -Ply serum and salivary IgG antibody levels were higher in children who were culture negative than those who were colonized. Antigen stimulation increased respective antigen-specific IgG production by adenoidal MNC and these responses were greater in those who were colonized than in culture-negative children. Antibodies to CbpA and Ply may protect children aged 2 years and older against pneumococcal colonization. Adenoids may be important local induction and effector sites for both mucosal and systemic antibody production to pneumococcal proteins in children.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

Section: Pneumococcal Culture Supernatants and Recombinant Proteinsmentioning

confidence: 99%

Section: Isolation Of Mnc From Adenoidsmentioning

confidence: 99%

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Serum and mucosal antibody responses to pneumococcal protein antigens in children:relationships with carriage status

et al. 2005

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“…To our knowledge, the present study provides the first confirmation that B cell memory is never established against linear epitopes of VP2 and adds further credence to the concept that long-lived plasma cells and memory B cells are 2 separate forms of independently controlled immunological memory [20,21]. A recent report of the enumeration, in the adenoids of children, of IgG memory B cells against 4 pneumococcal antigens has also shown a broad antigen-specific response, which suggests that certain antigens are more likely than others to elicit the establishment of B cell memory [22]. It is also likely that the B19-specific B cell ELISpot assay could find application in the confirmation of past infection with B19.…”

Section: Discussionmentioning

confidence: 99%

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1

Corcoran¹,

Mahon²,

Doyle³

2004

J INFECT DIS

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Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established against linear epitopes.Methods. B cell enzyme-linked immunospot assay was used to evaluate B19-specific B cell memory in volunteer donors ( ). n p 22 Results. B cell memory is maintained against conformational epitopes of VP2 and is absent against linear epitopes of VP2. Individuals seronegative for IgG against the unique region of VP1 have detectable B cell memory, with the potential to mount a humoral response on reexposure to B19. Conversely, in mice immunized with VP2, long-lasting IgG against linear epitopes of VP2 and a strong B cell-memory response are observed.Conclusions. B cell memory is established and maintained against conformational epitopes of VP2 and against linear epitopes of VP1 but not against linear epitopes of VP2. These findings further our understanding of the immune response to B19 and suggest that analysis of B19-specific B cell memory merits consideration for future B19-vaccine studies.

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Pneumococcal lipoproteins involved in bacterial fitness, virulence, and immune evasion

et al. 2016

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Edited by Wilhelm JustStreptococcus pneumoniae (pneumococcus) has evolved sophisticated strategies to survive in several niches within the human body either as a harmless commensal or as a serious pathogen causing a variety of diseases. The dynamic interaction between pneumococci and resident host cells during colonization of the upper respiratory tract and at the site of infection is critical for bacterial survival and the development of disease. Pneumococcal lipoproteins are peripherally anchored membrane proteins and have pivotal roles in bacterial fitness including envelope stability, cell division, nutrient acquisition, signal transduction, transport (as substrate-binding proteins of ABC transporter systems), resistance to oxidative stress and antibiotics, and protein folding. In addition, lipoproteins are directly involved in virulence-associated processes such as adhesion, colonization, and persistence through immune evasion. Conversely, lipoproteins are also targets for the host response both as ligands for toll-like receptors and as targets for acquired antibodies. This review summarizes the multifaceted roles of selected pneumococcal lipoproteins and how this knowledge can be exploited to combat pneumococcal infections.

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Immune Responses to Novel Pneumococcal Proteins Pneumolysin, PspA, PsaA, and CbpA in Adenoidal B Cells from Children

Cited by 62 publications

References 51 publications

Serum and mucosal antibody responses to pneumococcal protein antigens in children:relationships with carriage status

Serum and mucosal antibody responses to pneumococcal protein antigens in children:relationships with carriage status

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1

Pneumococcal lipoproteins involved in bacterial fitness, virulence, and immune evasion

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