Immunity in strain 2 guinea-pigs inoculated with vaccinia virus recombinants expressing varicella-zoster virus glycoproteins I, IV, V or the protein product of the immediate early gene 62

Lowry, Philip W.; Solem, Suzanne M.; Watson, Brandi N.; Koropchak, Celine M.; Thackray, Helen; Kinchington, Paul R.; Ruyechan, William T.; Ling, Paul D.; Hay, John; Arvin, Ann M.

doi:10.1099/0022-1317-73-4-811

Cited by 37 publications

(23 citation statements)

References 41 publications

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“…At this stage responses of non-VZV immune patients towards VZV-VLPs are unknown; however control VLPs (no VZV inserts) failed to induce substantial LPA responses in patients from this study, and it has been reported that VZV-antigens do not induce lymphocyte proliferation in non-immune patients and animals [Arvin et al, 1986;Giller et al, 1989;Watson et al, 1990;Lowry et al, 1992;Sato et al, 1998]. …”

Section: Discussionmentioning

confidence: 75%

Ability of yeast Ty-VLPs (virus-like particles) containing varicella-zoster virus (VZV)gE and assembly protein fragments to induce in vitro proliferation of human lymphocytes from VZV immune patients

Welsh¹,

Harper²,

Garcia-Valcarcel³

et al. 1999

J. Med. Virol.

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Yeast Ty virus-like particles (VLPs) containing viral protein inserts have previously been shown to be potent immunogens, inducing both humoral and cell mediated immunity (CMI). The antigenicity of hybrid VLPs containing fragments of the varicella-zoster virus (VZV) gE protein or the assembly protein (AP) was assessed by lymphocyte proliferation. Peripheral blood mononuclear cells (PBMCs) from patients with a recent natural VZV infection were stimulated in vitro with VZV-VLPs together with control antigens. PBMC samples from both varicella (85%) and zoster (75%) patients proliferated in responses to at least one of the gE VZV-VLPs. As reported for the first time, VZV specific lymphocyte responses were also identified towards the VZV AP in two varicella and two zoster patient samples. The results demonstrate specific CMI recognition of the VZV gE fragments tested and the VZV AP delivered in the form of recombinant Ty-VLPs, and highlights their potential use as a recombinant antigen delivery system for vaccination.

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Section: Discussionmentioning

confidence: 75%

Ability of yeast Ty-VLPs (virus-like particles) containing varicella-zoster virus (VZV)gE and assembly protein fragments to induce in vitro proliferation of human lymphocytes from VZV immune patients

Welsh¹,

Harper²,

Garcia-Valcarcel³

et al. 1999

J. Med. Virol.

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“…Several of these models have been employed, including the guinea pig (Arvin et al , 1987; Hayward et al , 1991; Lowry et al , 1993; Lowry et al , 1992; Matsunga et al , 1982; Myers et al , 1980; Myers and Stanberry, 1991; Myers et al , 1985; Myers et al , 1991), rat (Hasnie et al , 2007; Kennedy et al , 2001), mouse (Wroblewska et al , 1993), and cotton rat (Ambagala et al , 2010; Sato et al , 2003a; Xia et al , 2003). Although each of these has yielded information, none has, until now been entirely satisfactory.…”

Section: Discussionmentioning

confidence: 99%

Infected peripheral blood mononuclear cells transmit latent varicella zoster virus infection to the guinea pig enteric nervous system

Gan¹,

Wang²,

Chen³

et al. 2014

J. Neurovirol.

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Latent wild-type (WT) and vaccine (vOka) varicella-zoster virus (VZV) are found in the human enteric nervous system (ENS). VZV also infects guinea pig enteric neurons in vitro, establishes latency and can be reactivated. We therefore determined whether lymphocytes infected in vitro with VZV secrete infectious virions and can transfer infection in vivo to the ENS of recipient guinea pigs. T lymphocytes (CD3-immunoreactive) were preferentially infected following co-culture of guinea pig or human peripheral blood mononuclear cells with VZV-infected HELF. VZV proliferated in the infected T cells and expressed immediate early and late VZV genes. Electron microscopy confirmed that VZV-infected T cells produced encapsulated virions. Extracellular virus, however, was pleomorphic, suggesting degradation occurred prior to release, which was confirmed by the failure of VZV-infected T cells to secrete infectious virions. Intravenous injection of WT- or vOka-infected PBMCs, nevertheless, transmitted VZV to recipient animals (guinea pig > human lymphocytes). Two days post-inoculation, lung and liver, but not gut, contained DNA and transcripts encoding ORFs 4, 40, 66 and 67. Twenty-eight days after infection, gut contained DNA and transcripts encoding ORFs 4 and 66 but neither DNA nor transcripts could any longer be found in lung or liver. In situ hybridization revealed VZV DNA in enteric neurons, which also expressed ORF63p (but not ORF68p) immunoreactivity. Observations suggest that VZV infects T cells, which can transfer VZV to and establish latency in enteric neurons in vivo. Guinea pigs may be useful for studies of VZV pathogenesis in the ENS.

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“…Compared with unimmunized aniof the eye. mals [20], animals immunized with either VZV gE or gI were No animal species appears to be fully susceptible to VZV protected from subsequent challenge with VZV, as evidenced and, therefore, the full range of VZV disease has not been seen by a reduced induction of antibody to VZV open-reading frame 62 (ORF 62) protein, which was used as a marker of viral replication. , was passaged 10 times in guinea pig embryo fibroblasts.…”

mentioning

confidence: 99%

“…Each sample of the standard curve also contained 1 mg of genomic DNA nol. The cells were stimulated with 10 mg of purified gE or gI or with VZV antigens [20] consisting of sonicated VZV-infected derived from uninfected guinea pig eyes. Although controls containing ú10 3 copies of DNA were beyond guinea pig fibroblasts.…”

mentioning

confidence: 99%

Recombinant Varicella-Zoster Virus Glycoproteins E and I: Immunologic Responses and Clearance of Virus in a Guinea Pig Model of Chronic Uveitis

Kimura

Wang

Pesnicak

et al. 1998

Journal of Infectious Diseases

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Guinea pigs immunized with recombinant varicella-zoster virus (VZV) glycoproteins E (gE) and I (gI) developed antigen-specific antibodies in the sera, vitreous, and conjunctival washes. Sera from immunized animals neutralized both cell-free and cell-associated VZV, and peripheral blood lymphocytes proliferated in vitro in response to recombinant gE and gI and to antigens from VZV-infected cells. Immunized guinea pigs were inoculated intravitreally with VZV, which induces chronic uveitis. VZV DNA was more rapidly cleared and infectious VZV was isolated less frequently from the retinas of animals immunized with gE and gI compared with that in controls receiving adjuvant alone. Nonetheless, cellular infiltrates in the vitreous, retina, and choroid were prevalent 21 days after VZV inoculation in both the adjuvant-alone- and gE-gI-immunized animals. Immunization with VZV gE and gI induced potent humoral and cellular responses that accelerated the clearance of VZV DNA and may neutralize virus within the eye.

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Immunity in strain 2 guinea-pigs inoculated with vaccinia virus recombinants expressing varicella-zoster virus glycoproteins I, IV, V or the protein product of the immediate early gene 62

Cited by 37 publications

References 41 publications

Ability of yeast Ty-VLPs (virus-like particles) containing varicella-zoster virus (VZV)gE and assembly protein fragments to induce in vitro proliferation of human lymphocytes from VZV immune patients

Ability of yeast Ty-VLPs (virus-like particles) containing varicella-zoster virus (VZV)gE and assembly protein fragments to induce in vitro proliferation of human lymphocytes from VZV immune patients

Infected peripheral blood mononuclear cells transmit latent varicella zoster virus infection to the guinea pig enteric nervous system

Recombinant Varicella-Zoster Virus Glycoproteins E and I: Immunologic Responses and Clearance of Virus in a Guinea Pig Model of Chronic Uveitis

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