2007
DOI: 10.1016/j.jri.2006.08.001
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Immunization with autologous CD46 generates a strong autoantibody response in rats that targets spermatozoa

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Cited by 8 publications
(6 citation statements)
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“…Its specific expression in the epididymis and its demonstrated role in the initiation of sperm maturation makes Bin1b a promising candidate for male contraception. The present findings are consistent with several recent studies showing that inhibition of the epididymal ß-defensin molecules in the rat, mouse, or monkey through immunization can inhibit sperm function and fertility (5,(13)(14)(15) and indicating the potential of Bin1b as an immunocontraceptive.…”
Section: Figuresupporting
confidence: 93%
“…Its specific expression in the epididymis and its demonstrated role in the initiation of sperm maturation makes Bin1b a promising candidate for male contraception. The present findings are consistent with several recent studies showing that inhibition of the epididymal ß-defensin molecules in the rat, mouse, or monkey through immunization can inhibit sperm function and fertility (5,(13)(14)(15) and indicating the potential of Bin1b as an immunocontraceptive.…”
Section: Figuresupporting
confidence: 93%
“…This high-titre polyclonal antiserum was affinity-purified against non-conjugated peptide immobilised to an agarose gel support using a Sulfolink kit (Perbio Science, Cramlington, UK) and eluted with 100 mM glycine buffer (pH2.8). A rat anti-rat CD46 polyclonal antiserum generated using a recombinant fusion protein comprising IgG-Fc and rat SCR2-3 CD46 domains was a kind gift of B. P. Morgan, C. L. Harris and M. Mizuno, Cardiff University School of Medicine [ 17 ].…”
Section: Methodsmentioning
confidence: 99%
“…The immune antiserum gave an OD 405 nm 1.40G0.01 at 1:3200 dilution in ELISA against BSApeptide, whereas it was 0.11G0.01 at the same dilution against BSA alone; pre-immune serum gave OD 405 nm values of 0.13G0.01 and 0.12G0.01 at 1:3200 dilution against BSA-peptide and BSA alone respectively. We also included a polyclonal rat anti-rat CD46 antiserum (Mizuno et al 2007).…”
Section: Cd46 Protein Is Not Expressed In a Sylvaticus Testis Or Spermmentioning
confidence: 99%
“…A polyclonal antiserum raised against a recombinant rat CD46 SCR123 fusion protein by immunisation of female rats was a kind gift of B P Morgan, C L Harris and M Mizuno, Cardiff University School of Medicine (Mizuno et al 2007). Seven separate mouse monoclonal antibodies (MM1,2,3,4,5,8 and 9) raised against the rat CD46 SCR123 fusion protein (Mizuno et al 2004 were also a kind gift of B P Morgan, C L Harris and M Mizuno.…”
Section: Antibodiesmentioning
confidence: 99%
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