Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses

Paudel, Sarita; Easwaran, Maheswaran; Jang, Han Byul; Jung, Hyun‐Ju; Kim, Joo-Hun; Shin, Hyun‐Jin

doi:10.1016/j.virusres.2016.04.014

Cited by 8 publications

(2 citation statements)

References 35 publications

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“…The immunization of mice with pseudorabies virus incorporated chimera Fc has induced higher level of IgG, IgG1 and IgG2a and protected mice more efficiently from lethal challenge comparing to the immunization with normal inactivated virus (Takashima et al 2005). We previously reported that immunization of chicken with avian metapneumovirus MPV (aMPV) harboring chicken Fc induced higher level of antibodies and inflammatory cytokines (IFN-c and IL-1b) compared to those of aMPV (Paudel et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Immunization with porcine epidemic diarrhea virus harbouring Fc domain of IgG enhances antibody production in pigs

Park

Jang

Kim

et al. 2020

Veterinary Quarterly

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Background: Outbreaks of porcine epidemic diarrhea virus (PEDV) infection have reemerged and spread rapidly worldwide, resulting in significant economic losses. Vaccination is the best way to prevent PEDV infection in young piglets. Objective: To enhance the efficacy of an inactivated vaccine against PEDV, we evaluated the adjuvant properties of Fc domain of IgG. Methods: Fifteen crossbred gilts (180 $ 210 days old) were used. Five pigs in group 1 were intramuscularly vaccinated twice at 4 weeks and 2 weeks prior to farrowing with 10 6 TCID 50 of inactivated PEDV. Five pigs in group 2 were intramuscularly vaccinated twice at 4 weeks and 2 weeks prior to farrowing with 10 6 TCID 50 of inactivated PEDV-sFc. Five pigs in group 3 were not vaccinated and served as negative controls. Serum samples were collected at farrowing and subjected to ELISA, a serum neutralizing (SN) test, and a cytokine assay. Statistical analysis was performed by a two-tailed unpaired t-test. Results: Vero cells expressing swine IgG Fc on its surface was established. When PEDV was propagated in the cells expressing the swine Fc, PEDV virion incorporated the Fc. Immunization of pigs with inactivated PEDV harbouring Fc induced significantly higher antibody production against PEDV, comparing to the immunization with normal inactivated PEDV. In addition, we observed significantly increased IFN-c levels in sera. Conclusion: Our results indicate that Fc molecule facilitate immune responses and PEDV harbouring Fc molecule could be a possible vaccine candidate. However, a challenge experiment would be needed to investigate the protective efficacy of PEDV harbouring Fc.

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Section: Discussionmentioning

confidence: 99%

Immunization with porcine epidemic diarrhea virus harbouring Fc domain of IgG enhances antibody production in pigs

Park

Jang

Kim

et al. 2020

Veterinary Quarterly

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“…Infections by aMPV and Ornithobacterium rhinotracheale are one of the main problems related to the respiratory system in turkeys. Field cases and experimental studies have shown that the most common manifestations in cases of co-infection are airsacculitis and pneumonia [ 62 , 63 , 64 ].…”

Section: Co-infection Of Ampv and Bacteriamentioning

confidence: 99%

Trends and Challenges in the Surveillance and Control of Avian Metapneumovirus

Salles,

Pilati,

Muniz

et al. 2023

Viruses

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Among the respiratory pathogens of birds, the Avian Metapneumovirus (aMPV) is one of the most relevant, as it is responsible for causing infections of the upper respiratory tract and may induce respiratory syndromes. aMPV is capable of affecting the reproductive system of birds, directly impacting shell quality and decreasing egg production. Consequently, this infection can cause disorders related to animal welfare and zootechnical losses. The first cases of respiratory syndromes caused by aMPV were described in the 1970s, and today six subtypes (A, B, C, D, and two more new subtypes) have been identified and are widespread in all chicken and turkey-producing countries in the world, causing enormous economic losses for the poultry industry. Conventionally, immunological techniques are used to demonstrate aMPV infection in poultry, however, the identification of aMPV through molecular techniques helped in establishing the traceability of the virus. This review compiles data on the main aMPV subtypes present in different countries; aMPV and bacteria co-infection; vaccination against aMPV and viral selective pressure, highlighting the strategies used to prevent and control respiratory disease; and addresses tools for viral diagnosis and virus genome studies aiming at improving and streamlining pathogen detection and corroborating the development of new vaccines that can effectively protect herds, preventing viral escapes.

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Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections

Suarez¹,

Miller²,

Koch³

et al. 2019

Diseases of Poultry

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Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses

Cited by 8 publications

References 35 publications

Immunization with porcine epidemic diarrhea virus harbouring Fc domain of IgG enhances antibody production in pigs

Immunization with porcine epidemic diarrhea virus harbouring Fc domain of IgG enhances antibody production in pigs

Trends and Challenges in the Surveillance and Control of Avian Metapneumovirus

Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections

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