Immunization with Eimeria ninakohlyakimovae-live attenuated oocysts protect goat kids from clinical coccidiosis

Ruiz, Antonio; Muñoz, M. C.; Molina, José Molina; Hermosilla, Carlos; Andrada, M.; Lara, Pedro C.; Bordón, Elisa; Pérez, David; López, Antonio; Matos, L.; Guedes, A.; Falcón, Soraya; Falcón, Y. J. Mendoza; Martín, Sara María Martínez; Taubert, Anja

doi:10.1016/j.vetpar.2013.09.032

Cited by 24 publications

(18 citation statements)

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“…Such result indicated that UV irradiation of Eimeria oocysts succeeded in decreasing the infectivity and reproducibility of Eimeria species in immunized lambs with superiority of those exposed to one hour of irradiation. This results agreed with Zayan and El-Akabawy (2003) noticed that OPG was 40 fold (1 x 10 5 ) fewer in rabbit group inoculated by 1 x 10 4 X ray irradiated Eimeria stiedai than group (40 x 10 5 ) inoculated by 1x10 4 non irradiated oocysts before challenge, while no datable oocysts were observed after challenge in both groups, and Ruiz et al, (2014) who showed that Immunized group with X-ray irradiated E. ninakohlyakimovae oocysts in goat showed significantly reduced shedding of oocysts by 95.3% when compared to oocysts output induced by non-attenuated oocysts. While the course of re-challenge infection there was a significant reduction of oocysts shedding in animals immunized either with X rad-attenuated oocysts (87.7%) or non-attenuated oocysts (60.5%).…”

Section: -Discussionsupporting

confidence: 91%

“…Fecal samples were examined on 5 th to 20 th day post inoculation (DPI) for oocysts detection. Eimeria species oocysts were collected, isolated, counted and sporulated in 2.5% potassium dichromate at room temperature (Ruiz et al, 2014). The sporulated oocysts were identified according to Solusby (1982).…”

Section: A Collection and Propagation Of Eimeria Speciesmentioning

confidence: 99%

“…The use of γ radiation for attenuation of Eimeria species oocysts has been previously done in chicken by Gilbert et al, (1998), X ray in rabbits by Zayan and El-Akabawy (2003), X ray in goats by Ruiz et al (2014). However, no previous studies concerned with attenuation of sheep Eimeria species were used in immunization of sheep against coccidiosis despite of its sever pathogenic effect.…”

Section: Introductionmentioning

confidence: 99%

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Immunization of Lambs Against Coccidiosis by Using Ultraviolet Irradiated Eimeria Oocysts for the First Time

Ramadan

Elmadway

Lashin

et al. 2018

Benha Veterinary Medical Journal

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The study was conducted to study the effect of Ultraviolet radiated Eimeria oocysts on immune pattern of lambs. For this purpose, Eimeria sp. (E. crandalis, E. granulosa, E. ovinoidalis, E. parva, E. pallida and E. faurei) were collected from infected sheep and propagated in a lamb for increasing the number of oocysts. The collected Eimeria sp. from the lamb, were sporulated in potassium dichromate 2.5% and divided into 3 parts; the 1 st part was exposed to UV radiation for half hour and the 2 nd part for one hour and the third left without irradiation. Nine lambs were classified into 3 equal groups; G1 was inoculated by half hour UV irradiated Eimeria oocysts, G2 was inoculated by one-hour UV irradiated Eimeria oocysts and G3 was inoculated by non-irradiated Eimeria oocysts. Fecal samples were collected from all lamb groups from 5 th to 21 th days, identified and count. Challenge by non-attenuated Eimeria was carried out at 21 th day post inoculation. The obtained results revealed that immunization of lambs by using one-hour UV irradiated Eimeria oocysts showed low number of oocysts in feces before and after challenge compared to other groups. The same group also showed high level of γ globulin as well as increasing levels of IgG. It was recommended that immunization of lambs by one-hour UV irradiated Eimeria oocysts could protect lambs against Coccidiosis.

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Section: -Discussionsupporting

confidence: 91%

Section: A Collection and Propagation Of Eimeria Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunization of Lambs Against Coccidiosis by Using Ultraviolet Irradiated Eimeria Oocysts for the First Time

Ramadan

Elmadway

Lashin

et al. 2018

Benha Veterinary Medical Journal

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“…As infecções frequentemente ocorrem durante os primeiros dias após o nascimento e pode rapidamente conduzir a eliminação de oocistos, dependendo da duração média do período pré-patente das espécies de Eimeria envolvidas (RUIZ et al, 2014). O aparecimento de oocistos nas fezes dos cabritos com apenas 14 dias de idade indicou que a infecção ocorreu nos primeiros dias de vida, sugerindo infecção por espécies de Eimeria com período pré-patente curto (LIMA, 1981;DAI et al, 2006;RAKHSHANDEHROO et al, 2013;RUIZ et al, 2013).…”

Section: Resultsunclassified

Uso preventivo do toltrazuril para controle da coccidiose em cabritos de corte criados em região semiárida

Nunes

Cruz

Neto

2015

Rev. bras. saúde prod. anim.

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<p>Objetivou-se com este estudo avaliar a eficácia do toltrazuril no controle da coccidiose em cabritos de corte criados extensivamente em região semiárida e determinar a idade mais adequada para realização do tratamento. Quarenta cabritos foram separados por faixa etária e tratados com toltrazuril em dose única aos 14; 21; 28 e 35 dias de idade. Amostras de fezes foram coletadas semanalmente para determinação do número de oocistos por grama de fezes (Oopg) nas semanas subsequentes ao tratamento. Os animais também foram pesados semanalmente para determinação do ganho de peso médio diário (GPD). Todos os cabritos tratados não apresentaram oocistos nas fezes pelo menos por uma semana após o tratamento. O Oopg dos cabritos tratados, independentemente da idade do tratamento, foi menor do que os não tratados (P<0,05) ao longo de 10 -11 semanas pós-tratamento. O ganho de peso médio diário (GPD) foi similar em todos os tratamentos avaliados. O Oopg não foi influenciado pelo sexo, mas sim pela idade ao tratamento. Os animais tratados aos 21 dias de idade não apresentaram oocistos nas fezes por um período maior que os demais. O tratamento com toltrazuril realizado aos 21 dias de idade foi eficaz para controle da coccidiose em cabritos criados extensivamente no semiárido.</p>

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“…[65,66], and successful immunisation of goat kids with attenuated Eimeria spp. oocysts has been performed [67].…”

Section: Discussionmentioning

confidence: 99%

Controlled efficacy trial confirming toltrazuril resistance in a field isolate of ovine Eimeria spp.

et al. 2018

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BackgroundCoccidiosis due to Eimeria spp. infections in lambs causes increased mortality and substantial production losses, and anticoccidials are important for control of the infection. Anticoccidial resistance has been reported in poultry and swine, and we recently described reduced toltrazuril efficacy in ovine Eimeria spp. in some Norwegian sheep farms using a newly developed faecal oocyst count reduction test (FOCRT). The aim of the present study was to use a controlled efficacy trial to assess the efficacy of toltrazuril against a field isolate suspected of being resistant.MethodsTwenty lambs, 17–22 days old and raised protected against exposure to coccidia, were infected with a field isolate of 100,000 Eimeria spp. oocysts. This isolate was obtained from a farm with a previously calculated drug efficacy of 56% (95% confidence interval: -433.9 to 96.6%). At day 7 post-infection, 10 of the lambs were orally treated with 20 mg/kg toltrazuril (Baycox Sheep vet., Bayer Animal Health), while the other 10 lambs (controls) were given physiological saline. Clinical examinations were conducted, and weight gains recorded. Daily faecal samples were scored for diarrhoea on a scale from 1 to 5, and oocyst excretion was determined using a modified McMaster technique. Oocysts were morphologically identified to species level. At 17–24 days post-infection, the lambs were euthanized and necropsied.ResultsThe tested Eimeria isolate was resistant against toltrazuril, and resistance was seen in both pathogenic and non-pathogenic species. In addition, no significant differences in faecal score, growth, gross pathology or histological changes were identified between the two groups. The pathogenic E. ovinoidalis was the dominant species, and no significant difference in the individual prevalence of E. ovinoidalis post-treatment was found between treated (66.9%) and control lambs (61.9%). Other species identified included E. crandallis/weybridgensis, E. parva, E. marsica, E. faurei, E. pallida, E. ahsata and E. bakuensis.ConclusionsThis study confirms toltrazuril resistance in ovine Eimeria spp.; in addition, the data support the use of FOCRT as an appropriate tool for field evaluation of anticoccidial efficacy. Due to limited anticoccidial treatment alternatives, these findings may have important implications for the sheep industry, particularly in northern Europe.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-2976-4) contains supplementary material, which is available to authorized users.

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Immunization with Eimeria ninakohlyakimovae-live attenuated oocysts protect goat kids from clinical coccidiosis

Cited by 24 publications

References 42 publications

Immunization of Lambs Against Coccidiosis by Using Ultraviolet Irradiated Eimeria Oocysts for the First Time

Immunization of Lambs Against Coccidiosis by Using Ultraviolet Irradiated Eimeria Oocysts for the First Time

Uso preventivo do toltrazuril para controle da coccidiose em cabritos de corte criados em região semiárida

Controlled efficacy trial confirming toltrazuril resistance in a field isolate of ovine Eimeria spp.

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