Immunization with the recombinant myosin regulatory light chain (FhrMRLC) in Adjuplex® adjuvant elicits a Th1-biased immune response and a reduction of parasite burden in Fasciola hepatica infected rats

Buffoni, L.; Piva, Manoela Marchezan; Bąska, Piotr; Januszkiewicz, Kamil; Norbury, Luke J.; Prior, Keila Catarina; Dezen, Diogenes; Silva, A.S.; Wędrychowicz, H.; Mendes, Ricardo E.

doi:10.1016/j.parint.2019.102037

Cited by 9 publications

(8 citation statements)

References 47 publications

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“…Carbomer-based compounds (acrylic acid polymers) are under investigation as synthetic adjuvants, due to their ability to stimulate robust immunity and inherent low toxicity ( 25 ). They have been extensively studied in many preclinical models for vaccines against numerous pathogens, including parasites, viruses, and fungi ( 26 – 29 ).…”

Section: Resultsmentioning

confidence: 99%

Coimmunization with Preerythrocytic Antigens alongside Circumsporozoite Protein Can Enhance Sterile Protection against Plasmodium Sporozoite Infection

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Coimmunization with Preerythrocytic Antigens alongside Circumsporozoite Protein Can Enhance Sterile Protection against Plasmodium Sporozoite Infection

et al. 2023

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“…When administered with myosin regulatory light chain it induced strong protective Th1 immune responses in rats challenged with F . hepatica infection [ 79 ]. Similarly, naltrexone, an opioid receptor antagonist that can shift the immune response toward a Th1 profile induced strong protective Th1 immune responses in mice when administered with alum and F .…”

Section: Discussionmentioning

confidence: 99%

Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity

et al. 2020

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Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host. In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity. The FhEVs molecular cargo was defined using proteomic analysis and their surface topology characterised by glycan microarrays. The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites. Glycan arrays revealed surfaceexposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans. When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3). When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation. Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres. Thus, we have demonstrated that FhEVs induce a unique phentotype in DC capable of suppressing IL-2 secretion from T-cells. Our studies add to the growing immuno-proteomic database that will be an PLOS NEGLECTED TROPICAL DISEASES

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“…Helminth infections may lead to cognitive impairment [ 8 ], tissue damage [ 9 ], pregnancy complications [ 10 ], and numerous other symptoms and can sometimes be fatal [ 11 ]. The high prevalence, detrimental effects, and increasing drug resistance of helminths [ 12 , 13 ] have led to much research on developing vaccines [ 14 , 15 , 16 , 17 , 18 ]; however, despite promising results, there is still much to be done to achieve final success. Fully eradicating helminth infections may even in some aspects be controversial, since they may mitigate autoimmune diseases [ 19 , 20 , 21 ] and allergies [ 22 , 23 ] by dampening Th 1 and inducing Th 2 host immune responses [ 24 , 25 ].…”

Section: Helminthsmentioning

confidence: 99%

The Role of the Intestinal Epithelium in the “Weep and Sweep” Response during Gastro—Intestinal Helminth Infections

Bąska

Norbury

2022

Animals

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Helminths are metazoan parasites infecting around 1.5 billion people all over the world. During coevolution with hosts, worms have developed numerous ways to trick and evade the host immune response, and because of their size, they cannot be internalized and killed by immune cells in the same way as bacteria or viruses. During infection, a substantial Th2 component to the immune response is evoked which helps restrain Th1-mediated tissue damage. Although an enhanced Th2 response is often not enough to kill the parasite and terminate an infection in itself, when tightly coordinated with the nervous, endocrine, and motor systems it can dislodge parasites from tissues and expel them from the gut. A significant role in this “weep and seep” response is attributed to intestinal epithelial cells (IEC). This review highlights the role of various IEC lineages (enterocytes, tuft cells, Paneth cells, microfold cells, goblet cells, and intestine stem cells) during the course of helminth infections and summarizes their roles in regulating gut architecture and permeability, and muscle contractions and interactions with the immune and nervous system.

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Immunization with the recombinant myosin regulatory light chain (FhrMRLC) in Adjuplex® adjuvant elicits a Th1-biased immune response and a reduction of parasite burden in Fasciola hepatica infected rats

Cited by 9 publications

References 47 publications

Coimmunization with Preerythrocytic Antigens alongside Circumsporozoite Protein Can Enhance Sterile Protection against Plasmodium Sporozoite Infection

Coimmunization with Preerythrocytic Antigens alongside Circumsporozoite Protein Can Enhance Sterile Protection against Plasmodium Sporozoite Infection

Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity

The Role of the Intestinal Epithelium in the “Weep and Sweep” Response during Gastro—Intestinal Helminth Infections

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