Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin

Wright, David W.; McDaniels, Christopher N.; Swasdison, Somporn; Accavitti, Mary Ann; Mayne, Pauline M.; Mayne, Richard

doi:10.1016/0945-053x(94)90028-0

Cited by 17 publications

(11 citation statements)

References 29 publications

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“…This is not surprising, as each protein is thought to be a potential component of the elastic fiber superstructure. However, the fibrillins are also known to be deposited without elastin in some tissues, e.g., kidney and ciliary zonules (Wright et al, 1994). Indeed, we also observed the fibrillins and MAGPs in areas of the digital flexor tendon where elastin was not detected, i.e., the interior of the tendon body.…”

Section: Discussionsupporting

confidence: 54%

“…While co-localization of fibrillin 1 with fibrillin 2 often occurs, there are tissues in which expression does not overlap. For example, fibrillin 2 is thought to be exclusively expressed in auricular cartilage (Zhang et al, 1994), while fibrillin 1 may be the only fibrillin in the suspensory ligament of the lens (ciliary zonules) of the eye (Wright et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…Fibrillin-containing microfibrils are thought to serve as a framework for the deposition of elastin and thus forming the elastic fiber superstructure (Rosenbloom et al, 1993;Kielty and Shuttleworth, 1995). Fibrillin-containing fibers are also found without elastin in some tissues (Gibson and Cleary, 1987;Wright et al, 1994;Zhang et al, 1995).…”

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confidence: 99%

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Distribution of the elastic fiber and associated proteins in flexor tendon reflects function

2002

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The elastic fiber is known to be an important component of skin, lung, and vasculature. Much less is known about the distribution of elastin and elastic fiber-related proteins in connective tissues, yet genetic defects of elastic fiber constituents can lead to deficiencies in these tissues. For the first time, we determine the distribution of elastin, fibrillins 1 and 2, and microfibril-associated glycoproteins (MAGPs) 1 and 2 in the flexor digitorum profundus (FDP) tendon. Three functionally distinct regions of the FDP tendon, the fibrocartilagenous (FC) region, avascular/tensional (AV/T) region, and insertion region, were evaluated by immunohistochemical methods for these five proteins. Biochemical analysis of desmosine content, an elastin-specific cross-link, demonstrated the presence of elastin in each region, and this was verified histochemically. The fibrillins were found with elastin and also pericellularly with internal fibroblasts where elastin was not detected. Although there was overlapping distribution, fibrillin 2 was more prominent in the interior of the tendon while fibrillin 1 was prominent in outer cell layers that contained elastic fibers. Both MAGP-1 and -2 were found throughout the tendon, although the greatest abundance was near the tendon insertion to bone. Surprisingly, MAGP-1 demonstrated a filamentous appearance within the fibrocartilage that did not correspond to the fibrillin 1 or 2 or MAGP-2 staining pattern. Lastly, we have shown that a vincular membrane located along the dorsal surface of the tendon near the insertion has a very high elastin content and a unique interface with the tendon that consists of an elastic anchor within the tendon body. Anat Rec 268: 430 -440, 2002.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

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Distribution of the elastic fiber and associated proteins in flexor tendon reflects function

2002

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“…This might suggest that microfibrils are found exclusively in the sites where elastogenesis should take place; however, it has been shown that fibrillin microfibrils devoid of amorphous elastin also occur in nonelastic, fully developed tissues. Oxytalan fibers in the periodontal ligament (Fullmer and Lillie, 1958;Carmichael and Fullmer, 1966), lymphatic anchoring filaments (Leak and Burke, 1968), and suspensory ciliary ligament of the ocular lens (Inoue and Leblond, 1986;Wright et al, 1994) form bundles of elastin-free microfibrils. A fibrillar system in the dermis is also known to contain elastin-free microfibrils in its superficial region (Cotta-Pereira et al, 1976, 1977.…”

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confidence: 99%

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

et al. 2004

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In this study, a unique fiber system in the subectodermal mesenchyme of the chick limb bud was visualized immunohistochemically with the use of a novel monoclonal antibody termed "FB1." This antibody stained a subset of extracellular fibers in the embryonic mesenchyme. Among the fibers visualized, those running perpendicularly to the limb bud ectoderm became progressively prominent in their thickness and length, and organized into a parallel array in the subectodermal region. This fiber system was distinct from that of major collagens, fibronectin, or tenascin. A molecule immunoprecipitated with FB1 comigrated with JB3 antigen, or chicken fibrillin-2. The fibers visualized immunohistochemically by FB1 and JB3 were indistinguishable from each other, and ultrastructurally appeared to be bundles composed of tubular-like microfibrils that originated directly from the ectodermal basal lamina. They lacked the amorphous deposits that are characteristic of elastin. A similar array of subectodermal fibers was also found in the developing axilla and some truncal regions, again well before the development of a definitive dermis. These findings suggest that a parallel array of subectodermal FB1-positive fibers constitutes a precocious fiber system in the presumptive dermis prior to the substantial formation of collagenous fibers. These fibers could be developmentally linked to oxytalan fibers, which are known to be present in the papillary dermis in mature cutaneous tissue.

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“…Fibrillin polymers form the structural scaffold of extensible microfibrils1-3 which are present in ocular elastic tissues and are arranged in parallel bundles to form the zonular fibres 4. These fibrillin-rich microfibrils are morphologically identical to those which provide strength and long range elastic recoil to the connective tissues of blood vessels, lung, ligament, and dermis 5…”

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confidence: 99%

Fibrillin and the eye

Ashworth

Kielty²,

McLeod³

2000

British Journal of Ophthalmology

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Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin

Cited by 17 publications

References 29 publications

Distribution of the elastic fiber and associated proteins in flexor tendon reflects function

Distribution of the elastic fiber and associated proteins in flexor tendon reflects function

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

Fibrillin and the eye

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