Immuno-affinity columns versus conventional clean-up: a method-comparison study for the determination of zearalenone in corn

Schuhmacher, Rainer; Krska, Rudolf; Grasserbauer, M.; Edinger, William; Lew, H.

doi:10.1007/s002160050682

Cited by 41 publications

(22 citation statements)

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“…The ZON extraction procedure used was a down-scaled version of the previously described protocol (55). Briefly, 6 g of milled wheat kernels was homogenized and extracted in 24 ml of a mixture containing 75% (vol/vol) acetonitrile and 25% water by using an Ultra Turrax T25 mixer for 3 min at 24,500 rpm in a 50-ml Greiner disposable tube.…”

Section: Methodsmentioning

confidence: 99%

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A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

Mitterbauer

Weindorfer

Safaie

et al. 2003

Appl Environ Microbiol

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Zearalenone (ZON) is a nonsteroidal estrogenic mycotoxin produced by plant-pathogenic species of Fusarium. As a consequence of infection with Fusarium culmorum and Fusarium graminearum, ZON can be found in cereals and derived food products. Since ZON is suspected to be a cause of human disease, including premature puberty syndrome, as well as hyperestrogenism in farm animals, several countries have established monitoring programs and guidelines for ZON levels in grain intended for human consumption and animal feed. We developed a low-cost method for monitoring ZON contamination in grain based on a sensitive yeast bioassay. The indicator Saccharomyces cerevisiae strain YZRM7 is unable to grow unless an engineered pyrimidine biosynthetic gene is activated by the expressed human estrogen receptor in the presence of exogenous estrogenic substances. Deletion of the genes encoding ATP-binding cassette (ABC) transporters Pdr5p and Snq2p increases net ZON uptake synergistically. Less than 1 g of ZON per liter of medium is sufficient to allow growth of the indicator strain. To prevent interference with pyrimidines potentially present in biological samples, we also disrupted the genes FUR1 and URK1, blocking the pyrimidine salvage pathway. The bioassay strain YZRM7 allows qualitative detection and quantification of total estrogenic activity in cereal extracts without requiring further cleanup steps. Its high sensitivity makes this assay suitable for low-cost monitoring of contamination of maize and small grain cereals with estrogenic Fusarium mycotxins.

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Section: Methodsmentioning

confidence: 99%

“…The standard and the reference maize flour samples were prepared as part of a project to obtain certified reference materials for determination of the mycotoxin ZON in maize. Immunoaffinity cleanup and HPLC with fluorescence detection were used for ZON determination as described previously (55).…”

Section: Methodsmentioning

confidence: 99%

A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

Mitterbauer

Weindorfer

Safaie

et al. 2003

Appl Environ Microbiol

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“…HPLC gives higher sensitivity than thin layer chromatography (TLC) and it requires simpler clean-up work than those required by gas chromatography (GC) (Krska, 1998;Mateo, Mateo, Hinojo, Llorens, & Jimenez, 2002;Saeger, Sibanda, & van Peteghem, 2003;Schuhmacher, Krska, Grasserbauer, Edinger, & Lew, 1998;Visconti & Pascale, 1998). HPLC with fluorescence detection was accepted as the official first determination method of ZON by the AOAC (Ware, Zhao, Kuan, & Carman, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…HPLC with fluorescence detection was accepted as the official first determination method of ZON by the AOAC (Ware, Zhao, Kuan, & Carman, 1999). Immuno-affinity columns, which provide clean extracts and minimise the interference of co-extracted matrices, have been widely used for ZON analysis in sample matrices (Krska, 1998;Llorens et al, 2002;Mateo et al, 2002;Saeger et al, 2003;Schuhmacher et al, 1998;Silva & Vargas, 2001).…”

Section: Introductionmentioning

confidence: 99%

A limited survey of zearalenone in Indonesian maize-based food and feed by ELISA and high performance liquid chromatography

et al. 2005

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“…Like GC, it allows multi-analyte determination in a single chromatographic run. In this context, ZON, ZOLs [17,18] and ~-and fl-ZAL [ 16] have been analysed, attaining detection limits in the low ppb range. The major disadvantage of fluorescence and UV detectors, however, is the hecessity for an extensive and/or selective sample clean-up prior to the analysis, which facilitates the removal of the main parts of matrix components that may interfere with the analyte signal(s) and to preconcentrate the analyte(s) to reach the required low determination limits.…”

Section: Introductionmentioning

confidence: 99%

Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry

2000

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Key WordsColumn liquid chromatography Tandem mass spectrometry Zeranol, zearalenone and zearalenol SummaryAn LC-MS/MS method has been developed for the sensitive determination of the anabolic compound zeranol, its epimer taleranol and the mycotoxins zearalenone, azearalenol and fl-zearalenol in animal urine and meat samples. Sample preparation included extraction of meat samples and enzymatic digest of urine samples followed by solid phase extraction with RP-18 columns for sample clean-up. Mass spectrometric determination was carried out with an atmospheric pressure chemical ionisation interface (APCI) in the multi-reaction monitoring mode (MRM). Using the negative ion mode, a limit of detection between 0.1 and 0.5 ppb and a limit of determination between 0.5 and 1 ppb could be achieved. With zearalanone (ZAN) as internal standard, a linear range between 0.5 (1.0) and 100ppb in urine samples (cow, pig) and between 1 and 100 ppb in meat samples (cow, calf, pig) could be established. Depending on the biological matrix and analyte, standard deviations were below 8.5 %, with average recovery rates around 86 % to 102 % in spiked samples. The usefulness of the method developed was demonstrated via several contaminated cow and pig urine samples. the mycotoxins zearalenone (ZON) and e-zearalenol (7-ZOL) that can be carried over from contaminated feedstuffto animals [2][3][4]. ZON, e-ZOL and its epimer/%zearalenol (fl-ZOL) are produced by several Fusarium species that colonize grains [5]. High amounts can be found on maize, wheat, barley and oats. All three compounds exhibit distinct HC

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Immuno-affinity columns versus conventional clean-up: a method-comparison study for the determination of zearalenone in corn

Cited by 41 publications

References 0 publications

A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

A Sensitive and Inexpensive Yeast Bioassay for the Mycotoxin Zearalenone and Other Compounds with Estrogenic Activity

A limited survey of zearalenone in Indonesian maize-based food and feed by ELISA and high performance liquid chromatography

Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry

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