Immuno-Biosensing Based on Organic Electrochemical Transistors for Anti-Spike Protein Early Detection

Barra, Mario; Tomaiuolo, Giovanna; Villella, Valeria Rachela; Esposito, Speranza; D’Angelo, Pasquale; Cocuzza, Matteo; Bertana, Valentina; Camilli, Elena; Preziosi, Valentina

doi:10.2139/ssrn.4356125

“…After decades of development on both advanced organic mixed ionic‐electronic (semi)conductors (OMIECs) and novel device structures, excellent characteristics in OECTs, including low drive voltage (<1 V), high transconductance ( g m ), stable operation in physiological environments, and good logic circuit performance, have been exhibited. Therefore, they have unique advantages and great potential in the field of extremely sensitive biochemical sensors, neuromorphic devices, and implantable electronics [10–17] …”

Section: Introductionmentioning

confidence: 99%

Precisely Patterned Channels in a Vertical Organic Electrochemical Transistor with a Diazirine Photo‐Crosslinker

Lai,

Cheng,

Xie

et al. 2024

Angew Chem Int Ed

5

0

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Organic electrochemical transistors (OECTs) rely on both efficient ionic doping/de‐doping process and carrier transport in the mixed ionic‐electronic channel under the modulation of gate bias. Moreover, channels that hold photopatterning capability are highly desired to minimize parasitic capacitance and simplify the fabrication process/cost. However, yielding photo‐patternable channels with both precise/robust patterning capability and controllable ionic‐electronic coupling is still challenging. Herein, double‐end trifluoromethyl diazirines (DtFDA) with different chain lengths are introduced in the OECT channel to act as both photo‐crosslinker and medium to regulate ionic‐electronic transport. Specifically, high‐resolution patterns with a minimum line width/gap of 2 µm are realized in p(g2T‐T) or Homo‐gDPP based channels by introducing DtFDA. Maximum transconductances of 68.6 mS and 81.6 mS, current on/off ratio of 106 and 107 (under a drain voltage of only ±0.1 V), are achieved in p‐ and n‐type vertical OECTs (vOECTs), respectively, along with current densities exceeding 1 kA cm−2 and good cycling stability of more than 100,000 cycles (2000 seconds). This work provides a new and facile strategy for the fabrication of vOECT channels with high resolution and high performance via the introduction of a simple and efficient crosslinker.

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Precisely Patterned Channels in a Vertical Organic Electrochemical Transistor with a Diazirine Photo‐Crosslinker

Lai,

Cheng,

Xie

et al. 2024

Angewandte Chemie

0

View full text Add to dashboard Cite

Organic electrochemical transistors (OECTs) rely on both efficient ionic doping/de‐doping process and carrier transport in the mixed ionic‐electronic channel under the modulation of gate bias. Moreover, channels that hold photopatterning capability are highly desired to minimize parasitic capacitance and simplify the fabrication process/cost. However, yielding photo‐patternable channels with both precise/robust patterning capability and controllable ionic‐electronic coupling is still challenging. Herein, double‐end trifluoromethyl diazirines (DtFDA) with different chain lengths are introduced in the OECT channel to act as both photo‐crosslinker and medium to regulate ionic‐electronic transport. Specifically, high‐resolution patterns with a minimum line width/gap of 2 µm are realized in p(g2T‐T) or Homo‐gDPP based channels by introducing DtFDA. Maximum transconductances of 68.6 mS and 81.6 mS, current on/off ratio of 106 and 107 (under a drain voltage of only ±0.1 V), are achieved in p‐ and n‐type vertical OECTs (vOECTs), respectively, along with current densities exceeding 1 kA cm−2 and good cycling stability of more than 100,000 cycles (2000 seconds). This work provides a new and facile strategy for the fabrication of vOECT channels with high resolution and high performance via the introduction of a simple and efficient crosslinker.

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Sensing Characteristics of SARS-CoV-2 Spike Protein Using Aptamer-Functionalized Si-Based Electrolyte-Gated Field-Effect Transistor (EGT)

Shin,

Kim,

Choi

et al. 2024

Biosensors

1

0

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The sensing responses of SARS-CoV-2 spike protein using top-down-fabricated Si-based electrolyte-gated transistors (EGTs) have been investigated. An aptamer was employed as a receptor for the SARS-CoV-2 spike protein. The EGT demonstrated excellent intrinsic characteristics and higher sensitivity in the subthreshold regime compared to the linear regime. The limit of detection (LOD) was achieved as low as 0.94 pg/mL and 20 pg/mL for the current and voltage sensitivity, respectively. To analyze the sensing responses of EGT in detecting the aptamer–SARS-CoV-2 spike protein conjugate, a lumped-capacitive model with the presence of an effective dipole potential and an effective capacitance of the functionalized layer component was employed. The aptamer-functionalized EGT showed high sensitivity even in 10 mM phosphate-buffered saline (PBS) solution. These results suggest that Si-based EGTs are a highly promising method for detecting SARS-CoV-2 spike proteins.

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Immuno-Biosensing Based on Organic Electrochemical Transistors for Anti-Spike Protein Early Detection

Cited by 4 publications

References 0 publications

Precisely Patterned Channels in a Vertical Organic Electrochemical Transistor with a Diazirine Photo‐Crosslinker

Precisely Patterned Channels in a Vertical Organic Electrochemical Transistor with a Diazirine Photo‐Crosslinker

Precisely Patterned Channels in a Vertical Organic Electrochemical Transistor with a Diazirine Photo‐Crosslinker

Sensing Characteristics of SARS-CoV-2 Spike Protein Using Aptamer-Functionalized Si-Based Electrolyte-Gated Field-Effect Transistor (EGT)

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