Immuno field-effect transistor (ImmunoFET) for detection of salivary cortisol using potentiometric and impedance spectroscopy for monitoring heart failure

Halima, Hamdi Ben; Bellagambi, Francesca G.; Brunon, Fabien; Alcácer, Albert; Pfeiffer, Norman; Heuberger, Albert; Hangouet, Marie; Zine, Nadia; Bausells, Joan; Errachid, Abdelhamid

doi:10.1016/j.talanta.2022.123802

Cited by 16 publications

(11 citation statements)

References 48 publications

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“…Linear range, accuracy, precision, and limit of detection (LoD) of the IMFET were investigated to reach a preliminary validation of the device. Selectivity was confirmed by analysing TNF-α, cortisol [29], and the gold standard HF biomarker N-terminal pro-brain natriuretic peptide (NT-proBNP) samples [17]. Finally, our IL-10 concentration was quantified with our IMFET using the standard addition method (SAM) in real saliva samples.…”

Section: Introductionmentioning

confidence: 96%

A Novel IMFET Biosensor Strategy for Interleukin‐10 Quantification for Early Screening Heart Failure Disease in Saliva

et al. 2022

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We propose a salivary Interleukin-10 detection strategy as part of an easily integrable Lab-on-Chip and Point-of-Care IMFET for multi-detection Heart Failure (HF) biomarkers. Our developed IMFET showed good linearity between increasing IL-10 concentration and the charge transfer resistance in both standard solutions and real saliva samples with a LOD of 0.02 pg mL À 1 . More-over, the cross-selectivity study showed that the developed IMFET was highly selective towards IL-10 against other HF biomarkers. The precision of our IMFET was also evaluated, and the difference between the determined IL-10 concentration using the standard addition method and its expected one is < 20 %.

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Section: Introductionmentioning

confidence: 96%

A Novel IMFET Biosensor Strategy for Interleukin‐10 Quantification for Early Screening Heart Failure Disease in Saliva

et al. 2022

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“…The portable system features Bluetooth communication and user-friendly interfaces, making it compatible with digital health applications and providing on-site results in just 30 min. In addition, FETs have been used for sensing cortisol (Halima et al , 2023; Zhang and Jia, 2021), oxytocin (Ohshiro et al , 2022), human carbonic anhydrase 1 (CA1) (Kumar et al , 2020), NT-proBNP (Ben Halima et al , 2023), nitrate (Osaki et al , 2022), ammonium ions, potassium ions and calcium ions (Bao et al , 2019) in saliva.…”

Section: Electrochemical Techniquesmentioning

confidence: 99%

“…Salivary hormones and neurotransmitters are critical regulators of various physiological processes, such as growth, metabolism and stress response. Many works were conducted for cortisol sensing in saliva (Ertuğrul Uygun et al , 2020; Halima et al , 2023; Kämäräinen et al , 2018; Liu et al , 2020; Pasha et al , 2013; Rison et al , 2021; Venugopal et al , 2011; Zhang and Jia, 2021). Salivary cortisol testing offers a noninvasive, convenient and reliable method to measure cortisol levels, which play a crucial role in stress response and various physiological processes.…”

Section: Analytes In Saliva and Their Electrochemical Detectionmentioning

confidence: 99%

Electrochemical sensors for analyte in saliva: recent update

Li,

You,

Fan

et al. 2024

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Purpose This review provides an overview of recent advances in electrochemical sensors for analyte detection in saliva, highlighting their potential applications in diagnostics and health care. The purpose of this paper is to summarize the current state of the field, identify challenges and limitations and discuss future prospects for the development of saliva-based electrochemical sensors. Design/methodology/approach The paper reviews relevant literature and research articles to examine the latest developments in electrochemical sensing technologies for saliva analysis. It explores the use of various electrode materials, including carbon nanomaterial, metal nanoparticles and conducting polymers, as well as the integration of microfluidics, lab-on-a-chip (LOC) devices and wearable/implantable technologies. The design and fabrication methodologies used in these sensors are discussed, along with sample preparation techniques and biorecognition elements for enhancing sensor performance. Findings Electrochemical sensors for salivary analyte detection have demonstrated excellent potential for noninvasive, rapid and cost-effective diagnostics. Recent advancements have resulted in improved sensor selectivity, stability, sensitivity and compatibility with complex saliva samples. Integration with microfluidics and LOC technologies has shown promise in enhancing sensor efficiency and accuracy. In addition, wearable and implantable sensors enable continuous, real-time monitoring of salivary analytes, opening new avenues for personalized health care and disease management. Originality/value This review presents an up-to-date overview of electrochemical sensors for analyte detection in saliva, offering insights into their design, fabrication and performance. It highlights the originality and value of integrating electrochemical sensing with microfluidics, wearable/implantable technologies and point-of-care testing platforms. The review also identifies challenges and limitations, such as interference from other saliva components and the need for improved stability and reproducibility. Future prospects include the development of novel microfluidic devices, advanced materials and user-friendly diagnostic devices to unlock the full potential of saliva-based electrochemical sensing in clinical practice.

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“…They also demonstrate fast response times, enabling real-time or near-real-time monitoring of cortisol levels. Moreover, electrochemical sensors can be miniaturized, making them suitable for portable and point-of-care applications . Sensors can be cost-effective and compatible with complex biological samples, facilitating their integration into wearable devices or personalized healthcare systems.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Cortisol by Silver Nanoparticle-Modified Molecularly Imprinted Polymer-Coated Pencil Graphite Electrodes

et al. 2023

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The sensitive cortisol detection by an electrochemical sensor based on silver nanoparticle-doped molecularly imprinted polymer was successfully improved. This study describes the method development for cortisol detection in both aqueous solution and biological samples using molecularly imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-histidine methyl ester)-coated pencil graphite electrodes modified with silver nanoparticles (AgNPs) by differential pulse voltammetry (DPV). The cortisol-imprinted pencil graphite electrode (PGE) has a large surface area because of doped AgNPs with enhanced electroactivity. The prepared molecularly imprinted polymer was characterized by scanning electron microscopy. The DPV response of the synthesized electrode with outstanding electrical conductivity was clarified. Cortisol-imprinted polymer-coated PGEs (MIP), cortisol-imprinted polymer-coated PGEs with AgNPs (MIP@AgNPs), and nonimprinted polymer-coated PGEs with AgNPs (NIP@AgNPs) were evaluated for sensitive and selective detection of cortisol in aqueous solution. Five different cortisol concentrations (0.395, 0.791, 1.32, 2.64, and 3.96 nM) were applied to the MIP@AgNPs, and signal responses were detected by the DPV with a regression coefficient (R 2) value of 0.9951. The modified electrode showed good electrocatalytic activity toward cortisol for the linear concentration range from 0.395 to 3.96 nM, and a low limit of detection was recorded as 0.214 nM. The results indicate that the MIP@AgNPs sensor has great potential for sensitive and selective cortisol determination in biological samples.

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Immuno field-effect transistor (ImmunoFET) for detection of salivary cortisol using potentiometric and impedance spectroscopy for monitoring heart failure

Cited by 16 publications

References 48 publications

A Novel IMFET Biosensor Strategy for Interleukin‐10 Quantification for Early Screening Heart Failure Disease in Saliva

A Novel IMFET Biosensor Strategy for Interleukin‐10 Quantification for Early Screening Heart Failure Disease in Saliva

Electrochemical sensors for analyte in saliva: recent update

Electrochemical Detection of Cortisol by Silver Nanoparticle-Modified Molecularly Imprinted Polymer-Coated Pencil Graphite Electrodes

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