Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay

Abstract

We present a new rapid and easy-to-use immunoassay chip which we have named the immuno-pillar chip. It has hydrogel pillars, fabricated inside a microchannel, with many antibody molecules immobilized onto 1 µm diameter polystyrene beads. To evaluate the chip performance, we applied it to the sandwich assay of C-reactive protein (CRP), α-fetoprotein (AFP) and prostate-specific antigen (PSA), a cardiac and inflammation marker, tumors and prostate cancer markers, respectively. For detection of disease markers, we confirmed the chip provides rapid analysis (total assay time of about 4 min) with high sensitivity, it is easy-to-use (no special skills are needed), and it uses small volumes of the sample and reagent (0.25 µL each). Moreover, multiplex assay for three biomarkers was also possible. Additionally, the immuno-pillar chip has a big advantage of having hardly any influence on the assay results even if the introduction quantities of the sample or reagents are different.

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

“…For example, micropillars of hydrogel incorporating 1 μ m antibody coated particles fabricated inside a microchannel can be used for multi plexed immunoassays for the detection of C-reactive protein, α -fetoprotein and PSA. [ 224 ] The adsorption of proteins from solution onto hydrophobic surfaces can be exploited as a simple protein deposition method. [ 225 ] Antibodies can be patterned on PDMS using microfabricated capillary stencils, Figure 8 j.…”

Nano- and Microbiodevices for High-Performance Separation of Biomolecules

“…7 and 8 [8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Nanobiodevices are applied to diagnosis of hypertension by the fast analysis of SNPs (single nucleotide polymorphisims) of hypertension related genes and diagnosis of lung cancer through the detection of deletion of the specific sequence for the lung cancer related genes.…”

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

“…11,12 We previously developed immuno-pillar chips as a rapid, inexpensive, easy-touse, and highly sensitive chip applicable for multiplex assays. [13][14][15] Here we report the detection of cat Cys-C (cCys-C) using the immuno-pillar chips. The antibody-immobilized microbeads assembled in these pillars create efficient reaction sites for capturing cCys-C.…”