Immuno-spin trapping detection of antioxidant/pro-oxidant properties of zinc or selenium on DNA and protein radical formation via hydrogen peroxide

Abstract

Trace elements can participate in the catalysis of group-transfer reactions and can serve as their structural components. However, most of them including zinc and selenium have multifunctional roles in biological environments such as antioxidant and/or pro-oxidant effects, as concentration-dependent manner. Although it has been demonstrated the antioxidant actions of either selenium or zinc compounds, there are several documents pointing out their pro-oxidant/oxidant roles in biological systems. Here we have used ELISA-based immuno-spin trapping, a method for detection of free radical formation, to detect whether or not a zinc compound, Zn3(PO4)2, or a selenium compound, Na2SeO3, has antioxidant and/or pro-oxidant effect on 5,5-Dimethyl-1-Pyrroline-N-Oxide (DMPO)-DNA nitrone adducts induced with Cu(II)-H2O2-oxidizing system in in vitro preparations. Second, we examined whether this technique is capable to demonstrate the different DMPO-protein nitrone adduct productions in isolated protein crude of hearts from normal rats (CON) or rats with metabolic syndrome (MetS). Our data demonstrated that either Zn(2+) (100 µM) or SeO3(-2) (50 nM) has very strong antioxidant action against 200 µM H2O2-induced DMPO-DNA nitrone adduct production, whereas their higher concentrations have apparent pro-oxidant actions. We also used verification by Western blotting analysis whether immuno-spin trapping can be used to assess H2O2-induced DMPO-protein nitrone adducts in heart protein crudes. Our Western blot data further confirmed the ELISA-data from proteins and demonstrated how Zn(2+) or SeO3(-2) are dual-functioning ions such as antioxidant at lower concentrations while pro-oxidant at higher concentrations. Particularly, our present data with SeO3(-2) in DMPO-protein nitrone adducts, being in line with our previous observation on its dual-actions in ischemia/reperfusion-induced damaged heart, have shown that this ion has higher pro-oxidant actions over 50 nM in MetS-group compared to that of CON group.

Effect of Sodium Selenite on Pathological Changes and Renal Functions in Broilers Fed a Diet Containing Aflatoxin B1

“…The possible mechanisms of protective role of sodium selenite might be associated with following factors: (1) Se was the nucleus of antioxidant selenoenzymes which could alleviate oxidative-stress-associated kidney injury [ 42 ]. Indeed, AFB 1 -induced tissue injury was mediated through oxidative reaction [ 28 ]; (2) Se could reduce the formation of DNA adducts of aflatoxin in the chick [ 43 , 44 ]; and (3) Se could attenuate the decreased activity of Na + -K + ATPase induced by AFB 1 . Our result showed that Se could elevate AFB 1 -depressed Na + -K + ATPase activity which was accordance with the recent research [ 45 ].…”

Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy

“…The pioneering work for the development of IST was performed by the research group of Mason et In principle, all macromolecular radicals, derived from e.g., proteins or DNA, can be measured very specifically with IST. 451,[462][463][464][465][466][467][468][469] A novel and intriguing extension of the IST methodology arose from a combination with molecular magnetic resonance imaging (mMRI) for in-vivo and in-situ localization of radical sites in preclinical studies. In this case, chelated paramagnetic gadolinium (Gd), a well-known MRI contrast agent, is linked to a modified anti-DMPO.…”

The effect of gamma radiation on plasma levels of zinc and selenium in nuclear medicine staff

“…A number of previous studies have shown that zinc is required for many physiological processes including growth, neuronal function, reproduction, and immune system (5). Zinc deficiency, in this regard, has been implicated in a wide range of diseases, including immune system disorders, lymphopenia, intrinsic immunity, and increased infectious diseases (8,9). Selenium is the other essential element demanded for the body.…”