Immunoaffinity column clean-up techniques in food analysis: A review

Şenyuva, Hamide Z.; Gilbert, John

doi:10.1016/j.jchromb.2009.05.042

Cited by 145 publications

(66 citation statements)

References 201 publications

(172 reference statements)

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“…Column effiencies achieved in this study are comparable to other immunoaffinity SPE columns such as AFLASCAN, AFLA-RHONE, AflaTest, and AFLAPREP. For example, AflaTest column efficiencies for corn were calculated through the use of spiked sample extract and were: AFB 1 , AFG 1  90%, AFB 2  85%, and AFG 2  80% [27]. However, for the DDG SPE spiked extract efficiencies, they were lower than 75% suggesting that the aflatoxins are not being released from the column.…”

Section: Discussionmentioning

confidence: 99%

Effect of Matrix Clean-Up for Aflatoxin Analysis in Corn and Dried Distillers Grains

Mcdaniel¹,

Holmes²,

Armbrust³

et al. 2011

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In this study the effect of using an immunoaffinity column versus simple filtering as a cleanup was tested for aflatoxins extracted from corn and Dried Distillers Grains (DDG). The aflatoxins were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The use of an immunoaffinity column resulted in greater signal-to-noise ratios (S/N), S/N of 70 vs S/N of 5 for corn, as well as fewer non-target peaks in the analysis. Recoveries of aflatoxin using immunoaffinity ranged from

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Section: Discussionmentioning

confidence: 99%

Effect of Matrix Clean-Up for Aflatoxin Analysis in Corn and Dried Distillers Grains

Mcdaniel¹,

Holmes²,

Armbrust³

et al. 2011

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“…[17,28] The method investigated is accurate, selective and sensitive, thanks to highly effective clean up by IAC combined with LC-MS/MS technique. The absence of interfering components, the unambiguous identification of target molecules and the low DLs reached make it applicable to cereals and baby food official control.…”

Section: Discussionmentioning

confidence: 99%

“…[16] The availability of multi-mycotoxin IACs opens new frontiers for the analysis of these contaminants by food control laboratories, giving the opportunity of in-depth and exhaustive foodstuff monitoring. [17] However, the simultaneous determination of molecules with significantly different physicochemical properties requires a universal detection technique, such as LC coupled with mass spectrometry (MS). Several methods allowing for detecting and quantifying, by LC-MS/MS analysis, mycotoxins belonging to different classes in several kinds of food matrices, with one common sample preparation and a single chromatographic run, have recently been developed by various groups.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of deoxynivalenol, zearalenone, T‐2 and HT‐2 toxins in breakfast cereals and baby food by high‐performance liquid chromatography and tandem mass spectrometry

2010

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In this study, the simultaneous determination of deoxynivalenol (DON), zearalenone (ZEA), T-2 and HT-2 toxins in foodstuff was investigated. A new kind of multi-mycotoxin immunoaffinity columns (IACs) available on the market (DZT MS-PREP(®)) was tested. A sensitive, selective and accurate method by high-performance liquid chromatography and tandem mass spectrometry was developed, with electrospray ionization mass spectrometer operating in multiple reaction monitoring (MRM) mode, with negative-positive-negative ion switching. The method was used for the analysis of samples marked in Italy, in the frame of official monitoring plans. The advantages of combining IACs and LC-MS/MS technique are as follows: efficient removal of matrix interferences, simple chromatographic outline, high selectivity, low detection limits (DLs) and separation of a wide range of molecules with different physico-chemical properties in a single run. The method was studied on two different matrices, breakfast cereal and baby food, at contamination levels close to Regulation limits (EC) 1126/2007. The recoveries obtained (60-100%) fulfil the performance criteria required by Regulation (EC) 401/2006. The DL is 60 µg/kg for DON and 10 µg/kg for ZEA, T-2 and HT-2. Linearity range of the calibration curves is suitable for adult and baby food.

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“…Advances in sample preparation aim to minimize laboratory solvent use and hazardous waste production, save employee labor and time, and reduce the cost per sample, while improving the efficiency of the analyte isolation. This includes the development and application of molecularly imprinted polymers in food sample analysis [3], the use of monoliths in sample preparation and analysis of milk [4], the development of porous monolith microextraction techniques for determination of veterinary residues in food matrices by [5], the use of the so-called QuEChERS (quick, easy, cheap, effective, rugged, and safe) methodology for determining pesticide residues in food matrices [6], the application of immunoaffinity column clean-up techniques in food analysis [7], the development of solid-phase microextraction (SPME) techniques for quality characterization of food products [8], the application of ultrasound-assisted extraction to the determination of contaminants in food and soil samples [9], and the use of liquid phase microextraction in food analysis [10]. Besides, some papers have focused on the description of sample preparation strategies used for the analysis of aflatoxins in food and feed [11], antibacterial residues in foodstuffs [12], or the determination of pesticides in foods [13].…”

Section: Food Analysis: Current State Of the Art Methodologies And mentioning

confidence: 99%

Food Analysis: Present, Future, and Foodomics

Cifuentes

2012

ISRN Analytical Chemistry

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This paper presents a revision on the instrumental analytical techniques and methods used in food analysis together with their main applications in food science research. The present paper includes a brief historical perspective on food analysis, together with a deep revision on the current state of the art of modern analytical instruments, methodologies, and applications in food analysis with a special emphasis on the works published on this topic in the last three years (2009)(2010)(2011). The article also discusses the present and future challenges in food analysis, the application of "omics" in food analysis (including epigenomics, genomics, transcriptomics, proteomics, and metabolomics), and provides an overview on the new discipline of Foodomics.

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Immunoaffinity column clean-up techniques in food analysis: A review

Cited by 145 publications

References 201 publications

Effect of Matrix Clean-Up for Aflatoxin Analysis in Corn and Dried Distillers Grains

Effect of Matrix Clean-Up for Aflatoxin Analysis in Corn and Dried Distillers Grains

Simultaneous determination of deoxynivalenol, zearalenone, T‐2 and HT‐2 toxins in breakfast cereals and baby food by high‐performance liquid chromatography and tandem mass spectrometry

Food Analysis: Present, Future, and Foodomics

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