Immunoassay of nine serological tumor markers on a hydrogel-based microchip

Zubtsova, Zh. I.; Savvateeva, Elena; Butvilovskaya, V. I.; Tsybulskaya, M. V.; Chechetkin, V. R.; Samokhina, L. O.; Vinnitskiĭ, L I; Масленников, В. В.; Reznikov, Yu. P.; Заседателев, А. С.; Rubina, A. Yu.

doi:10.1134/s1068162013060101

Cited by 8 publications

(2 citation statements)

References 11 publications

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“…Detection of tumor markers AFP, HCG, CEA, CA 19–9, CA 125, and CA 15–3 in serum samples was performed on protein biochip by immunofluorescence analysis as described earlier . The study of 33 samples from the patients with CRC revealed increased concentration of CEA in 17% of patients, CA 19–9 in 13%, CA 125 in 17%, and CA 15–3 in 13%.…”

Section: Resultsmentioning

confidence: 99%

“…Here, we detected the levels of antibodies to glycans SiaTn, TF, Tn, Le C , Le Y , SiaLe A , and Man β 1‐4GlcNAc β as well as the level of the immunoglobulins IgG, IgA, and IgM using glycochip. Concentrations of protein cancer markers CEA, CA 19–9, CA 125, CA 15–3, HCG, and AFP were determined by immunofluorescence analysis using a protein biochip diagnostic test system . The 3D hydrogel biochips were manufactured using previously described technology that yields gel cells of uniform size and distribution of the probes within the cells, which is necessary for quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

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Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips

et al. 2016

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Colorectal cancer (CRC) is the third most common malignancy in industrialized countries. Despite the advances in diagnostics and development of new drugs, the 5‐year survival remains only 60–65%. Our approach to early diagnostics of CRC is based on the determination of serological signatures with an array of hemispherical hydrogel cells containing immobilized proteins and oligosaccharides (glycochip). The compounds immobilized on the glycochip include tumor‐associated glycans (SiaTn, Tn, TF, LeC, LeY, SiaLeA, and Manβ1‐4GlcNAcβ) and antibodies against human immunoglobulins IgG, IgA, and IgM. The glycochip detects antibodies against tumor‐associated glycans in patients’ sera. The simultaneous measurement of the levels of immunoglobulins enhances the diagnostic impact of the signatures. In this work, we found previously unreported increase in antibodies against oligosaccharide Manβ1‐4GlcNAcβ in patients with CRC. In parallel with these experiments, we determined the levels of oncomarkers carcinoembryonic antigen (CEA), cancer antigen (CA) 19–9, CA 125, CA 15–3, human chorionic gonadotropin (HCG), and alpha‐fetoprotein (AFP) using another gel‐based biochip with immobilized antibodies (oncochip) developed earlier in our laboratory. In total, 69 samples from healthy donors, 33 from patients with colorectal carcinoma, and 27 from patients with inflammatory bowel diseases were studied. The use of combined signatures of antiglycan antibodies and oncomarkers provides much better predictive value than the conventional measurement of oncomarkers CEA and CA 19–9. Positive predictive value of CRC diagnoses using together glycochip and oncochip reached 95% with the sensitivity and specificity 88% and 98%, respectively. Thus, the combination of antibody profiling with detection of conventional oncomarkers proved to be a promising tool in diagnostics of CRC.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips

et al. 2016

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Synthesis, reactions, and biological study of some new thienopyrimidine derivatives as antimicrobial and anti‐inflammatory agents

Tolba¹,

El‐Dean

Ahmed³

et al. 2018

J Chinese Chemical Soc

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Pyrimidine and thienopyrimidine derivatives play a very important role in organic chemistry because of their wide applications as bioactive compounds with multiple biological activities. However, a literature survey revealed that the merger of different groups in the thieno[2,3-d]pyrimidine heterocyclic ring enhances its antibacterial, antifungal and anti-inflammatory activities. This encouraged us to prepare a new series of thieno[2,3-d]pyrimidine heterocyclic compounds and to test them as antimicrobial and anti-inflammatory agents. These compounds have shown remarkable activity toward fungi, bacteria, and inflammation. Thus, these compounds have been prepared by the chloroacylation of 5-amino-4-phenyl-2-(p-tolylamino) thieno[2,3-d] pyrimidine-6-carboxamide (4) using chloroacetyl chloride under neat condition to afford the target compound (6), which was used as precursor for the synthesis of a number of bioactive compounds. Thus reaction of the chloromethylpyrimidine derivative (6) with triphenylphosphine in dry benzene gave the corresponding ((4-oxo-9-phenyl-7-(p-tolylamino)-3,4-dihydropyrimido[4 0 ,5 0 :4,5]thieno [2,3-d]pyrimidin-2-yl)methyl) triphenylphosphonium chloride (7). Compounds 8a-8c and 9a-9c were obtained by the reaction of 7 with some selected aromatic aldehydes and ketones in methanol and sodium methoxide under Wittig reaction condition. The structures of the all new synthesized compounds were established on the basis of their analytical and spectral data (IR, 1 H NMR, 13 C NMR, and MS).

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A Concise Review on the Synthesis and Reactions of Pyrazolopyrazine Heterocycles

ul‐Malik

Zaki

El‐Dean

et al. 2018

Journal of Heterocyclic Chem

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The pyrazolopyrazine ring system is a very interesting class of heterocyclic compounds that were considered as important scaffolds of many efficacious biologically active agents that show anticancer with low toxicity, antioxidant, anticonvulsant, antiparasitic, anti‐inflammatory, antiviral, antibacterial, antifungal, and analgesic activities. This review throws light on the detailed synthetic approaches for the synthesis and reactions of pyrazolopyrazine moiety. This follow up may encourage the chemists to generate new routes possessing pyrazolopyrazine nucleus with high efficacy.

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Immunoassay of nine serological tumor markers on a hydrogel-based microchip

Cited by 8 publications

References 11 publications

Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips

Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips

Synthesis, reactions, and biological study of some new thienopyrimidine derivatives as antimicrobial and anti‐inflammatory agents

A Concise Review on the Synthesis and Reactions of Pyrazolopyrazine Heterocycles

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