Immunoassay-type biosensor based on magnetic nanoparticle capture and the fluorescence signal formed by horseradish peroxidase catalysis for tumor-related exosome determination

Chen, Hao; Luo, Dan; Shang, Bing; Chen, Jianjun; Ji, Wei; Chen, Qinhua; Chen, Jishun

doi:10.1007/s00604-020-04275-x

Cited by 31 publications

(7 citation statements)

References 33 publications

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“…Also, improving the specificity of EV separation required for the high specificity of biomarkers represents another challenge. Appropriate modifications of magnetic nanoparticles by attaching biological probes, such as antibodies targeting EV surface markers, can efficiently improve specificity 517 , 518 .…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Integrative biology of extracellular vesicles in diabetes mellitus and diabetic complications

Liu¹,

Zhang²,

Tian³

et al. 2022

Theranostics

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Diabetes mellitus (DM) is a chronic systemic disease with increasing prevalence globally. An important aspect of diabetic pathogenesis is cellular crosstalk and information exchange between multiple metabolic organs and tissues. In the past decade, increasing evidence suggested that extracellular vesicles (EVs), a class of cell-derived membrane vesicles that transmit information and perform inter-cellular and inter-organ communication, are involved in the pathological changes of insulin resistance (IR), inflammation, and endothelial injury, and implicated in the development of DM and its complications. The biogenesis and cargo sorting machinery dysregulation of EVs may mediate their pathogenic roles under diabetic conditions. Moreover, the biogenesis of EVs, their ubiquitous production by different cells, their function as mediators of inter-organ communication, and their biological features in body fluids have generated great promise as biomarkers and clinical treatments. In this review, we summarize the components of EV generation and sorting machinery and highlight their role in the pathogenesis of DM and associated complications. Furthermore, we discuss the emerging clinical implications of EVs as potential biomarkers and therapeutic strategies for DM and diabetic complications. A better understanding of EVs will deepen our knowledge of the pathophysiology of DM and its complications and offer attractive approaches to improve the prevention, diagnosis, treatment, and prognosis of these disorders.

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Section: Conclusion and Perspectivementioning

confidence: 99%

Integrative biology of extracellular vesicles in diabetes mellitus and diabetic complications

Liu¹,

Zhang²,

Tian³

et al. 2022

Theranostics

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show abstract

“…After capturing the exosomes with anti-CD63, anti-EpCAM captured the exosomes derived from hepatic cancer. At this point, the oxidation–reduction reaction was triggered, and the fluorescent spectra at 370–550 nm were detected with a detection limit of 200 particles/mL [ 227 ].…”

Section: Tumor Exosomes For Cancer Detectionsmentioning

confidence: 99%

Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques

2021

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Exosomes, powerful extracellular nanovesicles released from almost all types of living cells, are considered the communication engines (messengers) that control and reprogram physiological pathways inside target cells within a community or between different communities. The cell-like structure of these extracellular vesicles provides a protective environment for their proteins and DNA/RNA cargos, which serve as biomarkers for many malicious diseases, including infectious diseases and cancers. Cancer-derived exosomes control cancer metastasis, prognosis, and development. In addition to the unique structure of exosomes, their nanometer size and tendency of interacting with cells makes them a viable novel drug delivery solution. In recent years, numerous research efforts have been made to quantify and characterize disease-derived exosomes for diagnosis, monitoring, and therapeutic purposes. This review aims to (1) relate exosome biomarkers to their origins, (2) focus on current isolation and detection methods, (3) discuss and evaluate the proposed technologies deriving from exosome research for cancer treatment, and (4) form a conclusion about the prospects of the current exosome research.

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“…Macromolecular structures with highly repetitive aliphatic chains such as dendrimers are very promising for immunoassay applications, cell biology ( e.g. , detoxification of biological fluids and cell separation), diagnosis ( e.g.…”

Section: Assembly Of Mnmps For Theranostic Applicationsmentioning

confidence: 99%

“…Macromolecular structures with highly repetitive aliphatic chains such as dendrimers are very promising for immunoassay applications, 132 cell biology (e.g., detoxification of biological fluids 133 and cell separation 134 ), diagnosis (e.g., as MRI contrast agents 135 ), and therapy (e.g., as hyperthermia agents 136 and as therapeutic nanocarriers and delivery systems 137 ). Because of their symmetric starlike structure, they overcome nonspecific or undertarget drug biodistribution, short circulation half-life, and undesired accumulation of the drug away from the target site.…”

Section: Assembly Of Mnmps For Theranostic Applicationsmentioning

confidence: 99%

Recent Advancements of Specific Functionalized Surfaces of Magnetic Nano- and Microparticles as a Theranostics Source in Biomedicine

Zheng

Schumacher

et al. 2021

ACS Biomater. Sci. Eng.

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Magnetic nano-and microparticles (MNMPs) belong to a highly versatile class of colloids with actuator and sensor properties that have been broadly studied for their application in theranostics such as molecular imaging and drug delivery. The use of advanced biocompatible, biodegradable polymers and polyelectrolytes as MNMP coating materials is essential to ensure the stability of MNMPs and enable efficient drug release while at the same time preventing cytotoxic effects. In the past years, huge progress has been made in terms of the design of MNMPs. Especially, the understanding of coating formation with respect to control of drug loading and release kinetics on the molecular level has significantly advanced. In this review, recent advancements in the field of MNMP surface engineering and the applicability of MNMPs in research fields of medical imaging, diagnosis, and nanotherapeutics are presented and discussed. Furthermore, in this review the main emphasis is put on the manipulation of biological specimens and cell trafficking, for which MNMPs represent a favorable tool enabling transport processes of drugs through cell membranes. Finally, challenges and future perspectives for applications of MNMPs as theranostic nanomaterials are discussed.

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Immunoassay-type biosensor based on magnetic nanoparticle capture and the fluorescence signal formed by horseradish peroxidase catalysis for tumor-related exosome determination

Cited by 31 publications

References 33 publications

Integrative biology of extracellular vesicles in diabetes mellitus and diabetic complications

Integrative biology of extracellular vesicles in diabetes mellitus and diabetic complications

Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques

Recent Advancements of Specific Functionalized Surfaces of Magnetic Nano- and Microparticles as a Theranostics Source in Biomedicine

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