Immunoblot assay to study the pemphigus vulgaris antigen in normal human epidermis and COLO-16 cell line

Mohimen, A; Ahmed, A. Razzaque

doi:10.1007/bf01112920

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…It is characterized by the presence of an IgG autoantibody that is targeted towards an antigen present in the intercellu lar cement substance [2], This antigen has now been identi fied to be a 130-kD protein present on the cell surface of epidermal cells belonging to the cadherin superfamily, probably a dcsmoglein [3], The antibodies are pathogenic and responsible for the clinical manifestation of the disease. This has been demon strated in studies in which the intraperitoneal injection of IgG from pemphigus sera produced disease in neonatal Balb/c mice [4].…”

Section: Introductionmentioning

confidence: 99%

Correlation of Subclasses of IgG with Disease Activity in Pemphigus vulgaris

Bhol

Mohimen²,

Ahmed³

1994

Dermatology

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Pemphigus vulgaris is an autoimmune blistering disease that is characterized by the presence of an antibody against an epidermal cell protein. In our previous studies, we have demonstrated that the presence of the antibody in the patients and in the healthy relatives of the patients strongly correlates with MHC haplotypes. The purpose of this study was to determine the subclasses of IgG present in the sera of patients with active disease, those in remission, healthy and unaffected relatives and normal controls. The presence of the autoantibody to the pemphigus antigen was assayed by a modified, sensitive immunoblot technique. The assay was considered positive if a 130-kD band was seen when normal human epidermis was used as a substrate. In patients with active disease, the sera contained antibodies of the IgG1 and IgG4 subclasses. The sera of patients in remission, those of healthy unaffected relatives and normal controls contained only the IgG1 subclass. The normal controls consist of MHC-matched and non-matched individuals. This study indicates that patients with disease activity have an antibody which is of the IgG1 and IgG4 subclasses, which we consider to be a pathogenic antibody. The sera of healthy relatives and normal controls that contain an antibody which binds to the pemphigus antigen is of the IgG1 subclass only and is considered to be a nonpathogenic or natural autoantibody. These observations provide the basis to study the immunoregulatory mechanisms and the production of normal and pathogenic antibodies.

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Section: Introductionmentioning

confidence: 99%

Correlation of Subclasses of IgG with Disease Activity in Pemphigus vulgaris

Bhol

Mohimen²,

Ahmed³

1994

Dermatology

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“…There are a number of reports on identification of epidermal autoantigens specific for each autoimmune blistering disease (1)(2)(3)(4)(5)(6). In our studies, the sera from patients with bullous pemphigoid, pemphigus vulgaris, and pemphigus foliaceus demonstrated differential antigenic patterns.…”

Section: Discussionmentioning

confidence: 65%

“…The immunoblotting assay for those diseases has been improved by several investigators (5,6). However, there are still difficulties in detection of autoantibody at low titers in patient sera.…”

Section: Introductionmentioning

confidence: 99%

“…The immunoblotting technique has been applied to studies which characterize autoantigens in autoimmune blistering diseases, such as bullous pemphigoid, pemphigus foliaceus, and pemphigus vulgaris and detect autoantibodies to the epidermis in the sera from such patients (1)(2)(3)(4)(5). The immunoblotting assay for those diseases has been improved by several investigators (5,6). However, there are still difficulties in detection of autoantibody at low titers in patient sera.…”

Section: Introductionmentioning

confidence: 99%

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