Immunochemical Identification of a New Molecular Form of Acid Phosphatase of Host Origin Arising during Infection of Potato Tubers by Phytophthora erythroseptica Pethybr.

Abstract

SUMMARYChanges in multiple forms of acid phosphatase from potato tubers during infection by Phytophthora erythroseptica were detected by polyacrylamide gel electrophoresis. In recently lifted tubers these changes included the appearance of a new molecular form of low electrophoretic mobility. Seasonal studies established that a similar molecular form developed in healthy tubers during storage. An antiserum to acid phosphatase from potato tubers was shown to be specific for the host enzyme. Utilizing a sectional immunofocusing procedure the novel molecular form from infected tissues cross-reacted with the antiserum, suggesting that this component originated from the host.