Immunochemical Studies on Blood Groups XXX. Cleavage of A, B, and H Blood-Group Substances by Alkali^*

Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose.

“…); polysaccharides from Klebsiella K7 (28), K21 (29), and K32 (30), Rhizobiura trifolii TA 1 (31), and types IV, XIV, and XXXII pneumococcal polysaccharides (32) (37), a heparin analogue from alginic acid (38), and chondroitin sulfate (Sigma Chemical Co., St. Louis, Mo. ); dextran (39) and various blood group B and I substances (40)(41)(42)(43)(44). Galaetose-l-phosphate, v-galactose-6-phosphate, and D-glucose-l-phosphate (Sigma Chemical Co.) were used as the potassium salts.…”

“…The contents of the tubes were mixed, placed at 37°C for 1 h, placed in the refrigerator for 1 wk and were mixed twice daily. The tubes were centrifuged, washed twice with saline, and analyzed for N by the ninhydrin method (44).…”

Identification of in vivo phosphorylated myosin subunits

“…1, 2 and 3. Protein determinations were made using either Lowry analyses [5] or determination of total nitrogen [6] as described in tables 1 and 2. Average of six determinations SE.…”

“…The samples were then reheated at 160°C for 1 hr. The tubes were cooled and 200 X of H, 0 and 100 cl1 of activated Ninhydrin [6] were added, heated at 95°C for 20 mm, cooled and brought to 10 ml with 50% ethanol. Nitrogen was analyzed at 570 nm against 50% ethanol blanks ]61. gel was electrophoresed in a boric acid buffer at pH 8.7; the running buffer was pH 8.2.…”

Studies on the Antigenic Activities of Yeasts

“…To the digests was added each 0.05 ml of 30% hydrogen peroxide and again heated at 160 C for 30 min. After cooling, 2 ml of water and 1 ml of activated ninhydrin reagent described by Schiffman et al [14] were added to each tube successively, and the mixture were heated at 95 C for 20 min. The mixtures were then diluted to 100 ml with 50% ethanol, and the color was measured at 570 mp, within 1 hr after completion of the color development.…”