Immunochromatographic Lateral Flow Strip Tests

Abstract

The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibodies, and haptens, such as drug residues. Here, we describe in detail the preparation of three examples of the strip tests for detection of (a) the infectious bursal disease virus; (b) Trichinella specific antibodies, and (c) Clenbuterol residues in urine samples.

Molecular diagnostics of potato infections with PVY and PLRV by immunochromatography

“…Дешевые, быстрые и простые аналитические технологии на основе им-мунохроматографии позволяют проводить высо-кочувствительные измерения без специальных на-выков и оборудования даже в полевых условиях. Являясь эффективным средством диагностирования, иммунохроматографические экспресс-тесты позво-ляют в течение нескольких минут определить и оценить содержание различных диагностически важных биологически активных веществ [6][7][8][9][10].…”

A highly sensitive and flexible magnetic nanoprobe labeled immunochromatographic assay platform for pathogen Vibrio parahaemolyticus

“…Although these methods are sensitive and specific, they are time-consuming, high skill level, and necessary for expensive equipment. Lateral flow immunochromatographic assay (LFIA) is an extended application of ELISA, it is rapid, simple, and appropriate for point-of-care test (Zhang et al, 2009;Dzantiev et al, 2014). The labels used in LFIA system mainly include colloidal gold nanoparticles (Kawatsu et al, 2006;Ju et al, 2010;Preechakasedkit et al, 2012;, magnetic nanoparticles Xu et al, 2009;Wang et al, 2015a), fluorescent microspheres (Xu et al, 2013), quantum dot and so on.…”

Covalent Conjugation of Antibody and Gold Nanoparticle for Development of Lateral Flow Immunoassay Test Strip

“…This technology offers several advantages such as simple, rapid and cost-effective. The format in LFA is based on the interaction of antigen and antibody on the surface of the nitrocellulose membrane (NC).The NCmembrane is immobilized with capture binding protein (antibody or antigen) [3]. Several different labels have been employed in LFA development such as colloidal gold nanoparticle, latex, nanocarbon [4].…”