Immunochromatography versus Conventional Culture Method for Detection of Campylobacter Jejuni and Campylobacter Coli in Gastroenteritis

Abstract

Campylobacter jejuni (C.jejuni) , Campylobacter coli (C.coli), modified charcoal cefoperazol dextrose agar(MccDA) Background:Campylobacter is an important food-borne diarrheal disease in the whole world. The use of cultureindependent diagnostic tests , such as stool antigen tests, for the detection of Campylobacter in stool is of particular interest.Objectives:This study aimed to compare between conventional culture method and Immunochromatography (I.C) For Detection Of C.jejuni and C. coli in Gastroenteritis at Benha University hospitals, and determine their antibiotic susceptibility pattern.Methodology: This study was carried out on 100 patients who developed gastroenteritis like symptoms attending the out patient clinic of Benha University Hospitals or admitted to the Pediatric and Internal medicine departments. Stool samples were cultured on modified charcoal cefoperazol dextrose agar (mCCDA) and were examined for Ag detection by I.C test using RIDA®QUICK kit .Antimicrobial susceptibility testing was performed for twelve different antibiotics by the Kirby-Bauer disc diffusion method. Results: Campylobacter was detected in 18% of samples by culture method. 72.2% of them were C. jejuni, while the other 27.8% were C. coli. Out of 18 positive by culture ,16(88.9%) were positive by I.C. However, out of 82 negative by culture , 2(2.4%) were positive by I.C. The diagnostic accuracy values of the RidaQuick Campylobacter® versus culture were: sensitivity of 88.9%, specificity of 97.6%, and positive and negativepredictive values of 88.9% and 97.6%, respectively. Conclusion: RIDAQUICK Campylobacter®provide a rapid and reliable alternative for conventional culture in laboratory diagnosis of enteric infections with C. jejuni and C. coli.