Immunocompetent cells and cytokine expression in the rat periodontal ligament at the initial stage of orthodontic tooth movement

Baba, So; Kuroda, Noriyuki; Arai, Chihiro; Nakamura, Yoshiki; Sato, Toshio

doi:10.1016/j.archoralbio.2010.11.010

Cited by 34 publications

(27 citation statements)

References 32 publications

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“…Previous in vivo studies have demonstrated that orthodontic tooth movement resulted in increased levels of pro-inflammatory cytokines in periodontal tissues (37,38). The present study confirmed an increase in IL-1β and TNF-α levels following exposure to tensile strength.…”

Section: Discussionmentioning

confidence: 99%

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Sun

Liu

Cen

et al. 2017

Molecular Medicine Reports

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The present study aimed to investigate the role of orthodontic force in osteogenesis differentiation, matrix deposition and mineralization in periodontal ligament cells (PDLCs) cells in inflammatory microenvironments. The mesenchymal origin of PDLCs was confirmed by vimentin and cytokeratin staining. PDLCs were exposed to inflammatory cytokines (5 ng/ml IL-1β and 10 ng/ml TNF-α) and/or tensile strength (0.5 Hz, 12% elongation) for 12, 24 or 48 h. Cell proliferation and tensile strength-induced cytokine expression were assessed by MTT assay and ELISA, respectively. Runt-related transcription factor 2 (RUNX2) and type I collagen (COL-I) expression were analysed by reverse transcription-quantitative polymerase chain reaction and western blot analysis. Additionally, alkaline phosphatase activity was measured, and the mineralization profile was evaluated by alizarin red S staining. PDLCs exposed to tensile strength in inflammatory microenvironments exhibited reduced proliferation and mineralization potential. Treatment with the inflammatory cytokines IL-1β and TNF-α increased RUNX2 expression levels; however, decreased COL-I expression levels, indicating that bone formation and matrix deposition involve different mechanisms in PDL tissues. Notably, RUNX2 and COL-I expression levels were decreased in PDLCs exposed to a combination of an inflammatory environment and loading strength. The decreased osteogenic potential in an inflammatory microenvironment under tensile strength suggests that orthodontic force may amplify periodontal destruction in orthodontic patients with periodontitis.

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Section: Discussionmentioning

confidence: 99%

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Sun

Liu

Cen

et al. 2017

Molecular Medicine Reports

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“…Genes related to wound healing were significantly strongly expressed. IL1 and IL8 are important mediators of the inflammatory response and play an important role in the induction of osteoclastic bone resorption during orthodontic tooth movement [21]. MMP3 and MMP9 are regulated by fibroblasts in the PDL and are induced in fibroblasts and immune cells that are recruited in the pathologic situation [22].…”

Section: Discussionmentioning

confidence: 99%

Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans

Lee

Kim

et al. 2013

PLoS ONE

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The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), while in the PDL it was the tumor-suppressor gene WIF1. Genes related to bone development and remodeling (STMN2, IBSP, BMP8A, BGLAP, ACP5, OPN, BMP3, and TM7SF4) and wound healing (IL1, IL8, MMP3, and MMP9) were also more strongly expressed in the PDL than in the dental follicle. In selected genes, a comparison among cDNA microarray, real-time reverse-transcription polymerase chain reaction, and immunohistochemical staining confirmed similar relative gene expressions. The gene-expression profiles presented here identify candidate genes that may enable differentiation between the dental follicle and PDL.

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“…The concentration of IL-1 mRNA in rats' periodontal ligament after orthodontic force loading is increased within 3 h, particularly on pressure side [7]. An inducted inflammatory process by perforating the buccal cortical plate of orthodontically treated rats showed higher levels of expression of inflammatory cytokines and their respective receptors [8]. In fact, administration of exogenous IL-1 Receptor Antagonist (IL-1RA) to treated mice leads to a 66% decrease in the levels of IL-1 when compared to the experimental tooth movement of vehicle treated mice, indicating a reduction of the number of osteoclasts on pressure side of periodontal tissues after histological characterization and a consequent downregulation of orthodontic tooth displacement rate in mice treated with IL-RA therapy [9].…”

Section: Proinflammatory Cytokines: Interleukin-1 (Il-1 ) Interleukimentioning

confidence: 99%

Biomarkers of Periodontal Tissue Remodeling during Orthodontic Tooth Movement in Mice and Men: Overview and Clinical Relevance

d’Apuzzo¹,

Nucci²,

Jamilian³

et al. 2017

Periodontitis - A Useful Reference

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Biologically active substances are expressed by cells within the periodontium in response to mechanical stimuli from orthodontic appliances. Several possible biomarkers representing biological modifications during specific phenomena as simile-inflammatory process, bone resorption and formation, periodontal ligament changes, and vascular and neural responses are proposed. Citations to potentially published trials were conducted by searching PubMed, Cochrane databases, and scientific textbooks. Additionally, hand searching and contact with experts in the area were undertaken to identify potentially relevant published and unpublished studies. Selection criteria were as follows: animal models involving only mice and rats undergoing orthodontic treatment; collection of gingival crevicular fluid (GCF) as a noninvasively procedure for humans; no other simultaneous treatment that could affect experimental orthodontic movement. The data suggest that knowledge of the remodeling process occurring in periodontal tissues during orthodontic and orthopedic therapies may be a clinical usefulness procedure leading to proper choice of mechanical stress to improve and to shorten the period of treatment, avoiding adverse consequences. The relevance for clinicians of evaluating the rate of some substances as valid biomarkers of periodontal effects during orthodontic movement, by means of two models of study, mice and men, is underlined.

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Immunocompetent cells and cytokine expression in the rat periodontal ligament at the initial stage of orthodontic tooth movement

Cited by 34 publications

References 32 publications

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans

Biomarkers of Periodontal Tissue Remodeling during Orthodontic Tooth Movement in Mice and Men: Overview and Clinical Relevance

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