Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation

Abstract

PURPOSE:To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO). METHODS:Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups: group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmospheres per 120 minutes, twice a day for three days and once a day for seven days. The groups were then subdivided in four subgroups: A15 (n=5), with euthanasia on the 15 th day; A45 (n=5), with euthanasia on the 45 th day; B15 (n=5) with euthanasia on the 15 th day and B45 with euthanasia on the 45 th day. Respectively on these days, fragments of the inferior pole of the spleen of all animals were collected and analyzed with the immunohistochemistry technique in order to evaluate PCNA expression. RESULTS:There was an expressive increase in PCNA immunoreactivity in the group B. The 45 day postoperative period resulted in a higher level of positivity than the 15 day postoperative period (p<0.01). CONCLUSION:The quantitative analysis of proliferating cell nuclear antigen positive suggests that hyperbaric oxygenation increases cellular proliferation, contributing to splenic regeneration.

Effect of total splenectomy in the lipid profile in mice

“…Studies with different experimental models showed significant changes in the lipid profile of the splenectomized animals 1,6,7,9,[19][20][21][22] . However, when part of the spleen is preserved, its viability is maintained [23][24][25] closer to the metabolic profile of humans due to the strong controversies and high morbidity and mortality rates caused by complications from the atherosclerotic disease.…”

NaCl pretreatment attenuates H.pylori-induced DNA damage and exacerbates proliferation of gastric epithelial cells (GES-1)

“…Ki67 antigen is an important biomarker for the assessment of tumor cell proliferation [ 11 ]. PCNA is a major biological index of cell proliferation, which can objectively reflect the proliferation of tumor cells [ 12 ]. The p21 protein is a cyclin-dependent kinase(cdk) inhibitory protein that functions as a cell cycle regulator to block the transition from G1 phase to S phase, thus suppressing cell proliferation [ 13 ].…”

Effects of inferior splenic lobe pole fixation and gastrosplenic peritoneal membrane section on the vitality of the remanent of subtotal splenectomy in rats

“…Considering the risks inherent to total splenectomy, the spleen's partial preservation is recommended 7,10,11,[17][18][19][20] .…”