Immunofluorescence studies on formalin-fixed and paraffin-embedded material

Witting, Ch.

doi:10.1016/s0005-8165(77)80084-x

Cited by 18 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We confirmed earlier reports showing the beneficial effects of enzyme pretreatment on tissue antigenicity of NBF-fixed sections (Huang et al, 1976;Denk et al, 1977;Witting 1977;Brozman, 1978;Qualman and Keren, 1979;Swoveland and Johnson, 1979;Johnson et al, 1980;Hondo et al, 1981). We have not seen any reports describing enzyme treatment of virus infected chicken tissues.…”

Section: Discussionsupporting

confidence: 92%

“…Our trypsin treatment was efficient in unmasking IBDV and IBV antigens although parts of tissues did not always adhere to the glass slides. Pronase digestion was carried out according to Witting (1977) and was shown to be especially efficient in revealing IBDV antigen. The epithelial and connective tissue components of trachea were damaged by pronase treatment, making identification of IBV impossible.…”

Section: Discussionmentioning

confidence: 99%

“…with 0.1% pronase (SIGMA VII) in phosphate buffer saline, pH 7.2 for 15 min at 37°C, then rinsed in PBS for 30 min (Witting, 1977); 2.…”

Section: Pretreatment Of Tissue Sections and Immunohistochemistrymentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin‐embedded chicken tissues

Jönsson

Engström

1986

Avian Pathology

View full text Add to dashboard Cite

SUMMARYImmunoperoxidase and immunofluorescent techniques were used to detect and localise infectious bursal disease virus (IBDV) and infectious bronchitis virus (IBV) in fixed, paraffin-embedded chicken tissues. A modified Bouin's solution (9 parts of 1% picric acid to 1 part of 10% neutral buffered formalin and 5% acetic acid) proved to be the fixative of choice. Tissues fixed in 10% neutral buffered formalin showed heavy background staining, whereas enzyme treatment of these tissues succeeded in exposing viral antigens and thus giving a specific reaction. For routine diagnostic purposes it will prove advantageous to detect IBDV and IBV in fixed, paraffin-embedded material.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin‐embedded chicken tissues

Jönsson

Engström

1986

Avian Pathology

View full text Add to dashboard Cite

show abstract

“…Sections were exposed for 90 min to 50 μM BdABK. Thereafter, slides were washed twice (1 min) in PIPES and fixed with 4% para-formaldehyde [ 36 ]. Slides were washed three times (5 min) and then exposed to 1 M of glycine for 90 min to eliminate autofluorescence from aldehyde-fixed tissue.…”

Section: Methodsmentioning

confidence: 99%

Cellular localization of kinin B1 receptor in the spinal cord of streptozotocin-diabetic rats with a fluorescent [Nα-Bodipy]-des-Arg9-bradykinin

Talbot

Théberge-Turmel

Liazoghli

et al. 2009

J Neuroinflammation

View full text Add to dashboard Cite

Background: The kinin B 1 receptor (B 1 R) is upregulated by pro-inflammatory cytokines, bacterial endotoxins and hyperglycaemia-induced oxidative stress. In animal models of diabetes, it contributes to pain polyneuropathy. This study aims at defining the cellular localization of B 1 R in thoracic spinal cord of type 1 diabetic rats by confocal microscopy with the use of a fluorescent agonist, [Nα-Bodipy]-des-Arg 9 -BK (BdABK) and selective antibodies.

show abstract

A histopathologic study of benign and malignant lymphoepithelial lesions of the parotid gland

Nagao¹,

Matsuzaki²,

Saiga³

et al. 1983

Cancer

View full text Add to dashboard Cite

Twenty‐three cases of benign and malignant lymphoepithelial lesions without Sjögren's syndrome were examined: 21 were classical benign lymphoepithelial lesions, and were mostly found in males older than 50 years of age. The other two cases were identified as undifferentiated carcinoma arising from benign lymphoepithelial lesion (8.7%; 2/23). Histologically, this carcinoma is characterized by the formation of cell nests of variable size and by marked lymphoplasmacytic infiltrations into the stromal tissue. In immunofluorescence, an increase of plasma cells containing IgA and IgG was noted in the marginal areas of carcinoma cell clusters. Electron microscopy disclosed that the carcinoma was composed of poorly differentiated cells with occasional squamous differentiation. No dysplastic characteristics were found in the infiltrating lymphocytic components. These two cases of carcinoma were found in middle‐aged females.

show abstract

Immunofluorescence studies on formalin-fixed and paraffin-embedded material

Cited by 18 publications

References 8 publications

Immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin‐embedded chicken tissues

Immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin‐embedded chicken tissues

Cellular localization of kinin B1 receptor in the spinal cord of streptozotocin-diabetic rats with a fluorescent [Nα-Bodipy]-des-Arg9-bradykinin

A histopathologic study of benign and malignant lymphoepithelial lesions of the parotid gland

Contact Info

Product

Resources

About