Immunogenic Properties of MVs Containing Structural Hantaviral Proteins: An Original Study

Shkair, Layaly; Garanina, Ekaterina; Martynova, Ekaterina; Kolesnikova, Alena Igorevna; Arkhipova, Svetlana S.; Titova, Angelina; Rizvanov, Albert A.; Khaiboullina, Svetlana

doi:10.3390/pharmaceutics14010093

Cited by 4 publications

(2 citation statements)

References 121 publications

(145 reference statements)

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“…Orthohantaviruses are zoonotic viruses that naturally persist in some reservoir species through prolonged infection. The mortality of HFRS is low overall but ranges from 0.43% to 15% depending on the infecting virus strain [47], and the mortality associated with HPS is 40-50% [48]. Europe records over 10,000 clinical cases of orthohantavirus disease each year, with case fatality rates varying from less than 1% to over 10% [49].…”

Section: Discussionmentioning

confidence: 99%

Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus

Sehgal,

Sharma,

Kaushal

et al. 2024

Viruses

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Puumala orthohantavirus (PUUV) is an emerging zoonotic virus endemic to Europe and Russia that causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome (HFRS). There are limited options for treatment and diagnosis of orthohantavirus infection, making the search for potential immunogenic candidates crucial. In the present work, various bioinformatics tools were employed to design conserved immunogenic peptides containing multiple epitopes of PUUV nucleocapsid protein. Eleven conserved peptides (90% conservancy) of the PUUV nucleocapsid protein were identified. Three conserved peptides containing multiple T and B cell epitopes were selected using a consensus epitope prediction algorithm. Molecular docking using the HPEP dock server demonstrated strong binding interactions between the epitopes and HLA molecules (ten alleles for each class I and II HLA). Moreover, an analysis of population coverage using the IEDB database revealed that the identified peptides have over 90% average population coverage across six continents. Molecular docking and simulation analysis reveal a stable interaction with peptide constructs of chosen immunogenic peptides and Toll-like receptor-4. These computational analyses demonstrate selected peptides’ immunogenic potential, which needs to be validated in different experimental systems.

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Section: Discussionmentioning

confidence: 99%

Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus

Sehgal,

Sharma,

Kaushal

et al. 2024

Viruses

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“…In another study, immunization with SEOV recombinant N protein induced high-titer antibody [ 216 ]. Recently, the efficacy of eliciting humoral and T cell immune response by delivery of PUUV Gn/Gc and N protein using microvesicles was demonstrated [ 217 ]. However, the efficacy of these recombinant proteins’ protection against lethal infection remains to be determined [ 218 ].…”

Section: Vaccines and Prevention Of Hantavirus Infectionsmentioning

confidence: 99%

Hemorrhagic Fever with Renal Syndrome in Asia: History, Pathogenesis, Diagnosis, Treatment, and Prevention

Sehgal

Mehta

Sahay

et al. 2023

Viruses

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Hemorrhagic Fever with Renal Syndrome (HFRS) is the most frequently diagnosed zoonosis in Asia. This zoonotic infection is the result of exposure to the virus-contaminated aerosols. Orthohantavirus infection may cause Hemorrhagic Fever with Renal Syndrome (HRFS), a disease that is characterized by acute kidney injury and increased vascular permeability. Several species of orthohantaviruses were identified as causing infection, where Hantaan, Puumala, and Seoul viruses are most common. Orthohantaviruses are endemic to several Asian countries, such as China, South Korea, and Japan. Along with those countries, HFRS tops the list of zoonotic infections in the Far Eastern Federal District of Russia. Recently, orthohantavirus circulation was demonstrated in small mammals in Thailand and India, where orthohantavirus was not believed to be endemic. In this review, we summarized the current data on orthohantaviruses in Asia. We gave the synopsis of the history and diversity of orthohantaviruses in Asia. We also described the clinical presentation and current understanding of the pathogenesis of orthohantavirus infection. Additionally, conventional and novel approaches for preventing and treating orthohantavirus infection are discussed.

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Cross‐reactivity of hantavirus antibodies after immunization with PUUV antigens

Shkair,

Sharma,

Hamza

et al. 2024

Biotech and App Biochem

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Nephropathia epidemica (NE), caused by Puumala (PUUV) orthohantavirus, is endemic in the Republic of Tatarstan (RT). There are limited options for NE prevention in RT. Currently, available vaccines are made using Haantan (HNTV) orthohantavirus antigens. In this study, the efficacy of microvesicles (MVs) loaded with PUUV antigens to induce the humoral immune response in small mammals was analyzed. Additionally, the cross‐reactivity of serum from immunized small mammals and NE patients with HNTV, Dobrava, and Andes orthohantaviruses was investigated using nucleocapsid (N) protein peptide libraries. Finally, the selected peptides were analyzed for allergenicity, their ability to induce an autoimmune response, and their interaction with Class II HLA. Several N protein peptides were found to be cross‐reactive with serum from MVs immunized small mammals. These cross‐reactive epitopes were located in oligomerization perinuclear targeting and Daxx‐interacting domains. Most cross‐reactive peptides lack allergenic and autoimmune reactivity. Molecular docking revealed two cross‐reacting peptides, N6 and N19, to have good binding with three Class II HLA alleles. These peptides could be candidates for developing vaccines and therapeutics for NE.

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Immunogenic Properties of MVs Containing Structural Hantaviral Proteins: An Original Study

Cited by 4 publications

References 121 publications

Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus

Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus

Hemorrhagic Fever with Renal Syndrome in Asia: History, Pathogenesis, Diagnosis, Treatment, and Prevention

Cross‐reactivity of hantavirus antibodies after immunization with PUUV antigens

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